Summary
Formaldehyde biodegradation by a strain of Pseudomonas putida has been studied. The results indicate that this biodegradation is initiated by a dismutation reaction, yielding as products formic acid and methanol. The degradation of methanol and formic acid begins after exhaustion of formaldehyde in the medium, and presents a diauxic pattern: first formic acid is consumed followed by methanol. Moreover, cell viability, which is affected by the amount of added formaldehyde, has been determined.
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References
Alexandersson R, Hedenstierna G, Kolmodin-Hedman B (1982) Exposure to formaldehyde: effects on pulmonary function. Arch Environ Health 37:279–284
Gerhardt P, Murray RGE, Costillon RN, Nester EW, Wood WA, Krieg NR, Philips GB (eds) (1981) Manual of methods for general bacteriology. American Society for Microbiology, Washington, pp 409–443
Grafstrom RC, Curren RD, Yang Li L, Harris CC (1985) Genotoxicity of formaldehyde in cultured human bronchial fibroblasts. Science 228:89–90
Hugh R, Gilardi GL (1980) Pseudomonas. In: Lennette EH, Spaulding EH, Truant JP (eds) Manual of clinical microbiology. American Society for Microbiology, Washington pp 250–269
Kato N, Shirakawa K, Kobayashi H, Sakazawa C (1983) The dismutation of aldehydes by a bacterial enzyme. Agric Biol Chem 47:39–46
Kato N, Kobayashi H, Shimao M, Sakazawa C (1984a) Properties of formaldehyde dismutation catalyzing enzyme of Pseudomonas putida F61. Agric Biol Chem 48:2017–2023
Kato N, Yamagami T, Kitayama K, Shimao M, Sakazawa C (1984b) Dismutation and cross dismutation of aldehydes, and alcohol: aldehyde oxidoreduction by resting cells of Pseudomonas putida F61 −a. J Biotechnol 1:295–273
Kirk RE, Othmer DF (1980) Encyclopedia of chemical technology, vol 11, 3rd edn. Wiley, New York, pp 236–250
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–273
Marsh GM (1982) Proportional mortality patterns among chemical plant workers exposed to formaldehyde. Br J Ind Med 39:313–322
Olsen JH, Dossing M (1982) Formaldehyde induced symptoms in day care centers. Am Ind Hyg Assoc J 43:366–370
Stainer RY, Palleroni NJ, Doudoroff M (1966) The aerobic pseudomonads: a taxonomic study. J Gen Microbiol 43:159–271
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Adroer, N., Casas, C., de Mas, C. et al. Mechanism of formaldehyde biodegradation by Pseudomonas putida . Appl Microbiol Biotechnol 33, 217–220 (1990). https://doi.org/10.1007/BF00176528
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DOI: https://doi.org/10.1007/BF00176528