Summary
The simultaneous effect of temperature and photon flux density on microalga Chlorella sp. growth was analysed by response surface methodology of two consecutive full factorial designs. Maximum specific growth rate was 0.128 h-1 at 35°C and 2400 μmol photon m-2s-1. A photoinhibition effect was observed at high photon flux densities with temperatures far below the optimum. Temperature was the main factor affecting specific growth rate.
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Bengoa-Ruigomez, M.V., Sanmartin, M., Hatziathanasiou, A., Divanach, P. and Kentouri, M. (1995). Abstract, submitted to V Congreso National de Acuicultura. Sant Carles de la Rápita, Spain, May 10–13th.
Deming, S.N. and Morgan, S.L. (1987). System theory. In Experimental design: a chemometric approach, pp 1–19, Amsterdam. Elsevier.
Fry, J.C. (1993). One-way analysis of variance. In: Biological Data Analysis. A practical Approac, J.C. Fry n ed. pp. 3–40, New York: Oxford University Press.
Glaude, R.M. and Maxey, J.E. (1994). J. Appl. Phycology, 6, 131–141.
Goldman, J.C. and Mann, R. (1980). J. Exp. Mar. Biol. Ecol., 46, 29–39.
Jensen, S. and Knutsen, G. (1993). J. Appl Phycology, 5, 495–504.
Montgomery, D.C. (1991). Métodos y diseños de superficie de respuesta In: Diseño y análisis de experimentos, pp. 467–509, Mexico: Grupo Editorial Iberoamérica S.A.
Richmond, A. (1990). Large scale microalgal culture and applications. In: Progress in Phycological Research, vol 7, Round and Chapma eds. pp. 269–329, Biopress Ltd.
Sattur, A.P. and Karanth, N.G. (1989). Biotech. Bioeng., 34, 868–871.
Thomson, P.A. and Guo, M. (1992). J. Phycol., 28, 481–488.
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García Sánchez, J.L., Sánchez Pérez, J.A., García Camacho, F. et al. Optimization of light and temperature for growing Chlorella sp. using response surface methodology. Biotechnol Tech 10, 329–334 (1996). https://doi.org/10.1007/BF00173248
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DOI: https://doi.org/10.1007/BF00173248