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Improvement of performance for cross-flow membrane filtration of pullulan broth

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Abstract

To improve the performance of cross-flow membrane filtration of pullulan broth from Aureobasidium pullulans, the effect of the cultivation conditions was examined. In particular, the sucrose concentration in the medium was changed over a wide range. By decreasing the sucrose concentration the distribution of morphology of the microbial cells in the broth changed; the yeast-like form became predominant and, as a result, the specific resistance of the microbial cake was lowered. When the broth was fermented with a sucrose concentration of 2.5% or lower, the filtration characteristics were greatly improved by periodic closure of permeation during cross-flow filtration.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Engineering, Okayama University, 700, Tsushima-naka, Okayama, Japan

    Hiroshi Yamasaki, Myung-Sup Lee, Takaaki Tanaka & Kazuhiro Nakanishi

Authors
  1. Hiroshi Yamasaki
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  2. Myung-Sup Lee
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  3. Takaaki Tanaka
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  4. Kazuhiro Nakanishi
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Additional information

On leave from Hayashibara Co., Ltd., Amase-minamimachi, Okayama 700, Japan

Correspondence to: K. Nakanishi

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Yamasaki, H., Lee, MS., Tanaka, T. et al. Improvement of performance for cross-flow membrane filtration of pullulan broth. Appl Microbiol Biotechnol 39, 21–25 (1993). https://doi.org/10.1007/BF00166842

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  • DOI: https://doi.org/10.1007/BF00166842

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