Abstract
The injection of B16F10 melanoma cells with recombinant human tumor necrosis factor alpha (TNF-α) into the tail vein of C57BL/6 mice resulted in 2- to 25-fold more metastatic foci in the lungs than the injection of tumor cells alone. Clearly, TNF-α significantly enhanced experimental tumor metastasis. Furthermore, it enhanced the metastasis of Lewis lung carcinoma cells. In contrast, a mutein of TNF-α, designated as F4236, having the cell-adhesive sequence (Tyr-Ile-Gly-Ser-Arg) at the N-terminus of the TNF molecule did not enhance metastasis, but rather exhibited similar antitumor activity to wild-type TNF-α in fibrosarcoma-bearing mice.
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Miyata, K., Kato, M., Shikama, H. et al. A YIGSR-containing novel mutein without the detrimental effect of human TNF-α of enhancing experimental pulmonary metastasis. Clin Exp Metast 10, 267–272 (1992). https://doi.org/10.1007/BF00133562
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DOI: https://doi.org/10.1007/BF00133562