Abstract
Moderately starved rotifers exhibited a two-phased increase in n-3 fatty acids when they were fed a diet rich in these fatty acids. The first 20–30 min of enrichment, the increase in n-3 fatty acids was primarily due to increased gut content. The subsequent slow increase was due to an incorporation of n-3 fatty acids into rotifers tissues. Saturation was achieved before 24 h of exposure and the saturation level was independent of the initial content of n-3 fatty acids in the rotifers.
Starvation and limited feeding of the enriched rotifers for additional 4–8 h at 10–20 °C did not affect the accumulated fatty acids significantly. This was found for rotifers with high and low initial content of n-3 fatty acids. The n-3 fatty acids were assimilated with high efficiency from the feed and were not metabolized faster than other groups of fatty acids.
Enriched rotifers retained their nutritional value for a sufficient period after enrichment to serve well as live feed for marine fish larvae.
Similar content being viewed by others
References
Bligh, E. G. & W. J. Dyer, 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37: 911–917.
Cowey, C. B., J. M. Owen, J. W. Adron & C. Middelton, 1976. Studies on the nutrition of marine flatfish. The effect of different diatary fatty acids on the growth and fatty acid composition of turbot (Scophthalmus maximus). Br. J. Nutr. 36: 479–486.
Ito, T., 1960. On the culture of the mixohaline rotifer Brachionus plicatilis O. F. Muller in the sea water. Rep. Fac. Fish Pref. Univ. Mie. 3: 708–740.
Korstad, J. E., O. Vadstein & Y. Olsen, 1988. Feeding kinetics of Brachionus plicatilis fed Isochrysis galbana. This volume.
Lubzens, E., 1987. Raising rotifers for use in aquaculture. Hydrobiologia. 147: 245–255.
Metcalfe, L. D., A. A. Schimtz & J. R. Pelka, 1966. Rapid preparation of fatty acid esters from lipids for gas chromatography. Analyt. Chem. 38: 514–515.
Owen, J. M., J. W. Adron, J. R. Sargent & C B. Cowey, 1972. Studies on the nutrition of marine flatfish. The effect of different diatary fatty acids on the tissue fatty acids of the plaice Pleuronectes platessa. Mar. Biol. 13: 160–166.
Watanabe, T., C. Kitajima & S. Fujita, 1983. Nutritional values of live food organisms used in Japan for mass propagation of fish: a review. Aquaculture. 34: 115–143.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Olsen, Y., Rodriguez Rainuzzo, J., Vadstein, O. et al. Kinetics of n-3 fatty acids in Brachionus plicatilis and changes in the food supply. Hydrobiologia 186, 409–413 (1989). https://doi.org/10.1007/BF00048939
Issue Date:
DOI: https://doi.org/10.1007/BF00048939