Abstract
A set-up for recording thermoluminescence emission together with the constant F0 fluorescence yield is described briefly. It is driven by a microcomputer through plugged-in cards.
Practical aspects of the simulation of TL bands and of decomposition of complex TL signals are examined. A reproducible and linear temperature gradient and the use of photon counting for luminescence detection are important features for further analyzing the recorded signal. The simulation procedure used is a step-by-step calculation of the number of charge recombinations, which is then substracted from the number of remaining charge pairs able to produce luminescence. This procedure consists first of a graphical fitting, followed by a numerical minimization, with a maximum of five simulated components. The quality of the simulation is evaluated by the sum of squares of differences (signal-simulation), related to the signal area. Equivalent decomposition patterns may be found for the same recording and additional information is needed for interpretation of TL data. Averaging signals is feasible, provided that maximum temperatures Tm of averaged bands are sufficiently similar (±3°C). Simultaneous measurement of the antenna fluorescence yield F0, using an ultra-weak pulsed blue LED, gives an estimate of the luminescence yield. This has to be taken into account in the analysis of the Q band and of high temperature (>40°C) bands.
The simulation parameters appear to be dependent on plant growth conditions. Quantitative analysis of thermoluminescence emission could be useful in the study of the effects of climatic factors on the photosynthetic apparatus in plants.
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Abbreviations
- PS-II:
-
Photosystem II
- TL:
-
thermoluminescence
- F0 :
-
constant fluorescence emission, under ultra-low light intensity
- QA and QB :
-
respectively, primary and secondary electron acceptors of Photosystem II
- S2 and S3 :
-
respectively, the two and three positively charged states of the oxygen evolving system
- SSD:
-
sum of squares of differences between the signal and a simulation (fitting) or between the signal and a smoothed curve (noise)
References
Arnold W and Azzi JR (1968) Chlorophyll energy levels and electron flow in photosynthesis. Proc Natl Acad Sci USA 61: 29–35
Bilger HW, Schreiber U and Lange OL (1984) Determination of leaf heat resistance: Comparative investigation of chlorophyll fluorescence changes and tissue necrosis methods. Oecologia 63: 256–262
Briantais JM, Ducruet JM, Hodges M and Krause GH (1991) The effects of low temperature acclimation and photoinhibitory treatments on Photosystem 2 studied by thermoluminescence and fluorescence decay kinetics. Photosynth Res 31: 1–10
Bukhov NG, Surendra Chandra Sabat and Prasanna Mohanty (1990) Analysis of chlorophyll a fluorescence changes in weak light in heat treated Amaranthus chloroplasts. Photosynth Res 23: 81–87
Demeter S, Vass I, Hideg E and Sallai A (1984) Comparative thermoluminescence study of triazine-resistant and-susceptible biotypes of Erigeron canadensis L. Biochim Biophys Acta 806: 16–24
DeVault D and Govindjee (1990) Photosynthetic glow peaks and their relationship with the free energy changes. Photosynth Res 24: 175–181
DeVault D, Govindjee and Arnold W (1983) Energetics of photosynthetic glow peaks. Proc Natl Acad Sci USA 80: 983–987
Ducruet JM and Lemoine Y (1985) Increased heat sensitivity of the photosynthetic apparatus in triazine-resistant biotypes from different plant species. Plant Cell Physiol 26: 419–429
Ducruet JM and Ort D (1988) Enhanced susceptibility of photosynthesis to high leaf temperature in triazine-resistant Solanum nigrum L. Evidence for photosystem II D1 protein site of action. Plant Science 56: 39–48
Ducruet JM and Mathis P (1991) Chlorophyll fluorescence transients in chloroplasts and leaves. In: Valenzano DP et al. (eds) Photobiological Techniques, chapter 11, pp 147–163. Plenum Press, New York
Etienne AL, Ducruet JM, Ajlani G and Vernotte C (1990) Comparative studies on electron transfer in Photosystem II of herbicide-resistant mutants from different organisms. Biochim Biophys Acta 1015: 435–440
Havaux M (1989) Comparison of atrazine-resistant and -susceptible biotypes of Senecio vulgaris L.: Effects of high and low temperature on the in vivo photosynthetic electron transfer in intact leaves. J Exp Bot 40 (217): 849–854
Lavorel J (1975) Luminescence. In: Govindjee (ed) Bioenergetics of Photosynthesis, pp 225–314. Academic Press, New York
Lavorel J and Joliot P (1972) A connected model of the photosynthetic unit. Biophysical J 12: 815–831
Miranda Prieto T and Ducruet JM (1992) Simultaneous measurement of thermoluminescence and fluorescence F0. Photosynthetica (in press)
Ohad I, Koike H, Shochat S and Inoue Y (1988) Changes in the properties of reaction centers during the initial stages of photoinhibition as revealed by thermoluminescence measurements. Biochim Biophys Acta 993: 288–298
Randall JR and Wilkins MHF (1945) Phosphorescence and electron traps I. The study of trap distribution II. The interpretation of long-period phosphorescence. Proc Roy Soc (London) series A 184: 366–408
Rutherford AW and Inoue Y (1984) Oscillation of delayed luminescence from PSII: Recombination of S2QB - and S3QB -. FEBS Lett 165: 163–170
Rutherford AW, Crofts AR and Inoue Y (1982) Thermoluminescence as a probe of Photosystem II photochemistry. The origin of the flash-induced glow peaks. Biochim Biophys Acta 682: 457–465
Sane PV and Rutherford AW (1986) Thermoluminescence in photosynthetic membranes. In: Govindjee, Fork DC and Amesz J (eds) Light Emission by Plants and Bacteria, pp 329–360. Academic Press, New York
Schreiber U and Armond PA (1978) Heat-induced changes of chlorophyll fluorescence in isolated chloroplasts and related heat-damage at pigment level. Biochim Biophys Acta 502: 139–151
Schreiber U, Colbow K and Vidaver W (1976) Analysis of temperature-jump chlorophyll fluorescence induction in plants. Biochim Biophys Acta 423: 249–263
Tatake VG, Desai TS, Govindjee and Sane PV (1981) Energy storage state of photosynthetic membranes: Activation energies and lifetimes of electron in the trap states by thermoluminescence method. Photochem Photobiol 33: 243–250
Vass I and Demeter S (1982) Classification of Photosystem II inhibitors by thermodynamic characterization of the thermoluminescence of inhibitor-treated chloroplasts. Biochim Biophys Acta 682: 496–499
Vass I and Inoue Y (1986) pH dependent stabilization of S2QinfA/su− and S2QfinB/su− charge pairs studied by thermoluminescence. Photosynth Res 10: 431–436
Vass I and Inoue Y (1992) Thermoluminescence in the study of Photosystem II. In: Barber J (ed) The Photosystems: Structure, Function and Molecular Biology, pp 259–294. Elsevier, Amsterdam
Vass I, Horvath G, Herczeg T and Demeter S (1980) Determination of activation energies and half-livers of thermoluminescence bands of chloroplasts applying the method of multicomponent curve resolution. FEBS Lett 116 (2): 293–297
Vass I, Horvath G, Herczeg T and Demeter S (1981) Photosynthetic energy conservation investigated by thermoluminescence. Activation energies and half-lives of thermoluminescence bands of chloroplasts determined by mathematical resolution of glow curves. Biochim Biophys Acta 634: 140–152
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Ducruet, JM., Miranda, T. Graphical and numerical analysis of thermoluminescence and fluorescence F0 emission in photosynthetic material. Photosynth Res 33, 15–27 (1992). https://doi.org/10.1007/BF00032979
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DOI: https://doi.org/10.1007/BF00032979