Abstract
Tree barks are considerable waste streams in forest industries, and a potentially interesting resource for biorefineries. Barks of six relevant tree species, namely poplar (Populus × canadensis), black locust (Robinia pseudoacacia), red oak (Quercus rubra), willow (Salix sp.), Corsican pine (Pinus nigra subsp. laricio), and larch (Larix decidua), were anatomically and chemically characterized. Anatomical analysis illustrated the structural heterogeneity of the different barks with occurrence of various cell types and their arrangement in phloem, periderm, and rhytidome. Summative chemical analysis showed a high extractive content (14–30 wt%) for all barks. Black locust bark presented a substantial suberin content (10 wt%). The lignin content was similar for most barks (22–29 wt%), except for Corsican pine bark (45 wt%). Analytical pyrolysis demonstrated that softwood bark lignin was mostly G type with a small amount of H units, whereas for hardwood species S, G, and H units were present, with low S/G ratios (0.3–0.7). The cell wall structural polysaccharides were rather low. Total monosaccharides ranged from 35 to 44 wt%, with glucose being predominant for almost all barks, followed by xylose in hardwood and mannose in softwood barks. Identification of the lipophilic extractives highlighted the predominance of resin acids for softwood barks, and fatty acids and triterpenoids for all barks. Analysis of the polar bark extracts revealed large variations in the content of phenolics (265–579 mg gallic acid equivalent/g extract), flavonoids (177–391 mg catechin equivalent/g extract), and condensed tannins (88–670 mg catechin equivalent/g extract). Furthermore, the polar extracts presented a high antioxidant potential (500–1209 mg trolox equivalent/g extract), as determined by the FRAP assay. Additionally, a very strong antioxidant activity (AAI > 2), as evaluated by the DPPH assay, was observed for all barks. In summary, the results highlight the marked anatomical and chemical interspecies variability in barks, thus suggesting the need for tailored biorefining approaches.
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Liao Y, Koelewijn S-F, Van den Bossche G, Van Aelst J, Van den Bosch S, Renders T, Navare K, Nicolaï T, Van Aelst K, Maesen M (2020) A sustainable wood biorefinery for low–carbon footprint chemicals production. Science 367:1385–1390
Ragauskas AJ, Williams CK, Davison BH, Britovsek G, Cairney J, Eckert CA, Frederick WJ, Hallett JP, Leak DJ, Liotta CL (2006) The path forward for biofuels and biomaterials. Science 311:484–489
Clark JH, Deswarte FEI, Farmer TJ (2009) The integration of green chemistry into future biorefineries. Biofuels Bioprod Biorefin 3:72–90
Isikgor FH, Becer CR (2015) Lignocellulosic biomass: a sustainable platform for the production of bio-based chemicals and polymers. Polym Chem 6:4497–4559
Corma A, Iborra S, Velty A (2007) Chemical routes for the transformation of biomass into chemicals. Chem Rev 107:2411–2502
Upton BM, Kasko AM (2016) Strategies for the conversion of lignin to high-value polymeric materials: review and perspective. Chem Rev 116:2275–2306
Delidovich I, Hausoul PJC, Deng L, Pfützenreuter R, Rose M, Palkovits R (2016) Alternative monomers based on lignocellulose and their use for polymer production. Chem Rev 116:1540–1599
Ghatak HR (2011) Biorefineries from the perspective of sustainability: feedstocks, products, and processes. Renew Sust Energ Rev 15:4042–4052
Harkin JM, Rowe JW (1971) Bark and its possible uses, (Research note FPL; 091): 56 p., 91.
Feng S, Cheng S, Yuan Z, Leitch M, Xu CC (2013) Valorization of bark for chemicals and materials: a review. Renew Sust Energ Rev 26:560–578
Stafford W, De Lange W, Nahman A, Chunilall V, Lekha P, Andrew J, Johakimu J, Sithole B, Trotter D (2020) Forestry biorefineries. Renew Energy 154:461–475
Evert RF (2006) Esau’s plant anatomy: meristems, cells, and tissues of the plant body: their structure, function, and development. John Wiley & Sons, Hoboken
Angyalossy V, Pace MR, Evert RF, Marcati CR, Oskolski AA, Terrazas T, Kotina E, Lens F, Mazzoni-Viveiros SC, Angeles G, Machado SR, Crivellaro A, Rao KS, Junikka L, Nikolaeva N, Baas P (2016) IAWA list of microscopic bark features. IAWA J 37:517–615
Rowell RM, Pettersen R, Han JS, Rowell JS, Tshabalala MA (2005) Cell wall chemistry, Handbook of wood chemistry and wood composites. CRC Press, Boca Raton, pp 35–74
Kurth EF (1947) The chemical composition of barks. Chem Rev 40:33–49
Delidovich I, Leonhard K, Palkovits R (2014) Cellulose and hemicellulose valorisation: an integrated challenge of catalysis and reaction engineering. Energy Environ Sci 7:2803–2830
Van de Vyver S, Geboers J, Jacobs PA, Sels BF (2011) Recent advances in the catalytic conversion of cellulose. ChemCatChem 3:82–94
Rinaldi R, Schüth F (2009) Acid hydrolysis of cellulose as the entry point into biorefinery schemes. ChemSusChem 2:1096–1107
Takkellapati S, Li T, Gonzalez MA (2018) An overview of biorefinery-derived platform chemicals from a cellulose and hemicellulose biorefinery. Clean Techn Environ Policy 20:1615–1630
Schutyser W, Renders T, Van den Bosch S, Koelewijn SF, Beckham GT, Sels BF (2018) Chemicals from lignin: an interplay of lignocellulose fractionation, depolymerisation, and upgrading. Chem Soc Rev 47:852–908
Rinaldi R, Jastrzebski R, Clough MT, Ralph J, Kennema M, Bruijnincx PC, Weckhuysen BM (2016) Paving the way for lignin valorisation: recent advances in bioengineering, biorefining and catalysis, Angew. Chem Int Ed 55:8164–8215
Sun Z, Fridrich B, de Santi A, Elangovan S, Barta K (2018) Bright side of lignin depolymerization: toward new platform chemicals. Chem Rev 118:614–678
Azadi P, Inderwildi OR, Farnood R, King DA (2013) Liquid fuels, hydrogen and chemicals from lignin: a critical review. Renew Sust Energ Rev 21:506–523
Renders T, Van den Bossche G, Vangeel T, Van Aelst K, Sels B (2019) Reductive catalytic fractionation: state of the art of the lignin-first biorefinery. Curr Opin Biotechnol 56:193–201
Galkin MV, Samec JS (2016) Lignin valorization through catalytic lignocellulose fractionation: a fundamental platform for the future biorefinery. ChemSusChem 9:1544–1558
Koumba-Yoya G, Stevanovic T (2017) Transformation of sugar maple bark through catalytic organosolv pulping. Catalysts 7:294
Liu L-Y, Patankar SC, Chandra RP, Sathitsuksanoh N, Saddler JN, Renneckar S (2020) Valorization of bark using ethanol–water organosolv treatment: isolation and characterization of crude lignin. ACS Sustain Chem Eng 8:4745–4754
Romaní A, Larramendi A, Yáñez R, Cancela Á, Sánchez Á, Teixeira JA, Domingues L (2019) Valorization of Eucalyptus nitens bark by organosolv pretreatment for the production of advanced biofuels. Ind Crop Prod 132:327–335
Neiva DM, Costa RA, Gominho J, Ferreira-Dias S, Pereira H (2020) Fractionation and valorization of industrial bark residues by autohydrolysis and enzymatic saccharification. Biores Technol Rep 11:100441
Torget R, Himmel ME, Grohmann K (1991) Dilute sulfuric acid pretreatment of hardwood bark. Bioresour Technol 35:239–246
Normark M, Winestrand S, Lestander TA, Jönsson LJ (2014) Analysis, pretreatment and enzymatic saccharification of different fractions of Scots pine. BMC Biotechnol 14:20
Vangeel T, Renders T, Van Aelst K, Cooreman E, Van den Bosch S, Van den Bossche G, Koelewijn S-F, Courtin C, Sels B (2019) Reductive catalytic fractionation of black locust bark. Green Chem 21:5841–5851
Kumaniaev I, Samec JSM (2018) Valorization of Quercus suber bark toward hydrocarbon bio-oil and 4-ethylguaiacol. ACS Sustain Chem Eng 6:5737–5742
Garrett MD, Bennett SC, Hardacre C, Patrick R, Sheldrake GN (2013) New methods in biomass depolymerisation: catalytic hydrogenolysis of barks. RSC Adv 3:21552–21557
Pereira H (2007) Cork: biology, production and uses. Elsevier, Amsterdam
Leite C, Pereira H (2017) Cork-containing barks—a review. Front Mater 3:63
Şen A, Miranda I, Santos S, Graça J, Pereira H (2010) The chemical composition of cork and phloem in the rhytidome of Quercus cerris bark. Ind Crop Prod 31:417–422
Cardoso S, Ferreira J, Quilhó T, Pereira H (2017) Cork of Douglas-fir bark: impact of structural and anatomical features on usage. Ind Crop Prod 99:135–141
Ferreira JP, Quilhó T, Pereira H (2017) Characterization of Betula pendula outer bark regarding cork and phloem components at chemical and structural levels in view of biorefinery integration. J Wood Chem Technol 37:10–25
Mota GS, Sartori CJ, Ferreira J, Miranda I, Quilhó T, Mori FA, Pereira H (2016) Cellular structure and chemical composition of cork from Plathymenia reticulata occurring in the Brazilian Cerrado. Ind Crop Prod 90:65–75
Sousa AF, Gandini A, Silvestre AJ, Pascoal Neto C (2008) Synthesis and characterization of novel biopolyesters from suberin and model comonomers. ChemSusChem 1:1020–1025
Kumaniaev I, Navare K, Mendes NC, Placet V, Van Acker K, Samec JS (2020) Conversion of birch bark to biofuels. Green Chem 22:2255–2263
Fengel D, Wegener G (1983) Wood: chemistry, ultrastructure, reactions. Walter de Gruyter, Berlin
Neiva DM, Rencoret J, Marques G, Gutiérrez A, Gominho J, Pereira H, del Rio JC (2020) Lignin from tree barks: chemical structure and valorization. ChemSusChem 13:4537–4547
Miranda I, Lima L, Quilhó T, Knapic S, Pereira H (2016) The bark of Eucalyptus sideroxylon as a source of phenolic extracts with anti-oxidant properties. Ind Crop Prod 82:81–87
Franceschi VR, Krokene P, Christiansen E, Krekling T (2005) Anatomical and chemical defenses of conifer bark against bark beetles and other pests. New Phytol 167:353–376
Rohdewald P (2002) A review of the French maritime pine bark extract (Pycnogenol), a herbal medication with a diverse clinical pharmacology. Int J Clin Pharmacol Ther 40:158–168
Nahrstedt A, Schmidt M, Jäggi R, Metz J, Khayyal MT (2007) Willow bark extract: the contribution of polyphenols to the overall effect. Wien Med Wochenschr 157:348–351
Neiva DM, Luís Â, Gominho J, Domingues F, Duarte AP, Pereira H (2020) Bark residues valorization potential regarding antioxidant and antimicrobial extracts. Wood Sci Technol:1–27
Salem MZM, Elansary HO, Elkelish AA, Zeidler A, Ali HM, Mervat E-H, Yessoufou K (2016) In vitro bioactivity and antimicrobial activity of Picea abies and Larix decidua wood and bark extracts. BioResources 11:9421–9437
Arbenz A, Averous L (2015) Chemical modification of tannins to elaborate aromatic biobased macromolecular architectures. Green Chem 17:2626–2646
Pizzi A (2008) Tannins: major sources, properties and applications, Monomers, polymers and composites from renewable resources. Elsevier, Amsterdam, pp 179–199
Lacoste C, Čop M, Kemppainen K, Giovando S, Pizzi A, Laborie M-P, Sernek M, Celzard A (2015) Biobased foams from condensed tannin extracts from Norway spruce (Picea abies) bark. Ind Crop Prod 73:144–153
Hokkanen HMT, Ahlnäs T, Alakurtti S, Demidova N, Fuchs J, Izotov D, Kleeberg H, Koskimies S, Langat M, Lynch J (2012) ForestSpeCs findings on byproducts of forest industry: could bark be more valuable than timber? In: 4th Nordic Wood Biorefinery Conference, NWBC 2012. VTT Technical Research Centre of Finland, Espoo, pp 56–59
Tharakan P, Volk T, Abrahamson L, White E (2003) Energy feedstock characteristics of willow and hybrid poplar clones at harvest age. Biomass Bioenergy 25:571–580
Sitzia T, Cierjacks A, De Rigo D, Caudullo G (2016) Robinia pseudoacacia in Europe: distribution, habitat, usage and threats. European Atlas of Forest Tree Species. Publication office of the European Union, Luxembourg, pp 166–167
Vansteenkiste D, De Boever L, Van Acker J (2005) Alternative processing solutions for red oak (Quercus rubra) from converted forests in Flanders, Belgium, Proceedings of the COST Action E44 Conference on Broad Spectrum Utilization of Wood at BOKU Vienna, Austria, June 14-15, 2005. Universität für Bodenkultur Wien, Vienna, pp 13–26
Enescu C, de Rigo D, Caudullo G, Mauri A, Houston Durrant T (2016) Pinus nigra in Europe: distribution, habitat, usage and threats. Euro Atlas Forest Tree Spec 6:126–127
Da Ronch F, Caudullo G, Tinner W, de Rigo D (2016) Larix decidua and other larches in Europe: distribution, habitat, usage and threats
Brus DJ, Hengeveld GM, Walvoort DJJ, Goedhart PW, Heidema AH, Nabuurs GJ, Gunia K (2012) Statistical mapping of tree species over Europe. Eur J For Res 131:145–157
Pividori M, Giannetti F, Barbati A, Chirici G (2016) European Forest Types: tree species matrix. Publ. Off, Luxembourg
Pollet C, Jourez B, Hebert J (2008) Natural durability of black locust (Robinia pseudoacacia L.) wood grown in Wallonia, Belgium. Can J For Res 38:1366–1372
Ball J, Carle J, Del Lungo A (2005) Contribution of poplars and willows to sustainable forestry and rural development. Unasylva 56:3–9
Vallet P, Meredieu C, Seynave I, Bélouard T, Dhôte J-F (2009) Species substitution for carbon storage: Sessile oak versus Corsican pine in France as a case study. For Ecol Manag 257:1314–1323
Barbosa AC, Pace MR, Witovisk L, Angyalossy V (2010) A new method to obtain good anatomical slides of heterogeneous plant parts. IAWA J 31:373–383
Neiva DM, Araújo S, Gominho J, Carneiro AdC, Pereira H (2018) An integrated characterization of Picea abies industrial bark regarding chemical composition, thermal properties and polar extracts activity. PLoS One 13:e0208270
Miranda I, Gominho J, Mirra I, Pereira H (2012) Chemical characterization of barks from Picea abies and Pinus sylvestris after fractioning into different particle sizes. Ind Crop Prod 36:395–400
Neiva DM, Araújo S, Gominho J, Carneiro AdC, Pereira H (2018) Potential of Eucalyptus globulus industrial bark as a biorefinery feedstock: chemical and fuel characterization. Ind Crop Prod 123:262–270
Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16:144–158
Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555–559
Sun B, Ricardo-da-Silva JM, Spranger I (1998) Critical factors of vanillin assay for catechins and proanthocyanidins. J Agric Food Chem 46:4267–4274
Scherer R, Godoy HT (2009) Antioxidant activity index (AAI) by the 2, 2-diphenyl-1-picrylhydrazyl method. Food Chem 112:654–658
Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239:70–76
Marques AV, Pereira H (2013) Lignin monomeric composition of corks from the barks of Betula pendula, Quercus suber and Quercus cerris determined by Py–GC–MS/FID. J Anal Appl Pyrolysis 100:88–94
Faix O, Meier D, Fortmann I (1990) Thermal degradation products of wood. Holz Roh Werkst 48:281–285
Ralph J, Hatfield RD (1991) Pyrolysis-GC-MS characterization of forage materials. J Agric Food Chem 39:1426–1437
Crivellaro A, Schweingruber FH (2013) Atlas of wood, bark and pith anatomy of Eastern Mediterranean trees and shrubs: with a special focus on Cyprus. Springer Science & Business Media, Berlin
Schweingruber FH, Steiger P, Börner A (2019) Bark anatomy of trees and shrubs in the temperate Northern Hemisphere. Springer, Berlin
Howard ET (1977) Bark structure of southern upland oaks. Wood Fiber Sci 9:172–183
Nunes E, Quilhó T, Pereira H (1996) Anatomy and chemical composition of Pinus pinaster bark. IAWA J 17:141–150
Nunes E, Quilhó T, Pereira H (1999) Anatomy and chemical composition of Pinus pinea L. bark. Ann For Sci 56:479–484
Trockenbrodt M (1991) Qualitative structural changes during bark development in Quercus robur, Ulmus glabra, Populus tremula and Betula pendula. IAWA J 12:5–22
Whitmore T (1963) Studies in systematic bark morphology: IV. The bark of beech, oak and sweet chestnut. New Phytol 62:161–169
Şen AU, Quilho T, Pereira H (2011) Bark anatomy of Quercus cerris L. var. cerris from Turkey. Turk J Bot 35:45–55
Quilho T, Sousa V, Tavares F, Pereira H (2013) Bark anatomy and cell size variation in Quercus faginea. Turk J Bot 37:54
Gričar J, Jagodic Š, Prislan P (2015) Structure and subsequent seasonal changes in the bark of sessile oak (Quercus petraea). Trees 29:747–757
Oktaee J, Lautenschläger T, Günther M, Neinhuis C, Wagenführ A, Lindner M, Winkler A (2017) Characterization of willow bast fibers (Salix spp.) from short-rotation plantation as potential reinforcement for polymer composites. BioResources 12:4270–4282
Yemele MCN, Koubaa A, Cloutier A, Soulounganga P, Wolcott M (2010) Effect of bark fiber content and size on the mechanical properties of bark/HDPE composites. Compos A: Appl Sci Manuf 41:131–137
Sillero L, Prado R, Andrés MA, Labidi J (2019) Characterisation of bark of six species from mixed Atlantic forest. Ind Crop Prod 137:276–284
Harun J, Labosky P (2007) Chemical constituents of five northeastern barks. Wood Fiber Sci 17:274–280
Kofujita H, Ettyu K, Ota M (1999) Characterization of the major components in bark from five Japanese tree species for chemical utilization. Wood Sci Technol 33:223–228
Dou J, Galvis L, Holopainen-Mantila U, Reza M, Tamminen T, Vuorinen T (2016) Morphology and overall chemical characterization of willow (Salix sp.) inner bark and wood: toward controlled deconstruction of willow biomass. ACS Sustain Chem Eng 4:3871–3876
Yoon S-Y, Kim B-R, Han S-H, Shin S-J (2015) Different response between woody core and bark of goat willow (Salix caprea L.) to concentrated phosphoric acid pretreatment followed by enzymatic saccharification. Energy 81:21–26
Bryers RW (1996) Fireside slagging, fouling, and high-temperature corrosion of heat-transfer surface due to impurities in steam-raising fuels. Prog Energy Combust Sci 22:29–120
Pereira H, Miranda I, Tavares F, Quilhó T, Graça J, Rodrigues J, Shatalov A, Knapic S (2011) Qualidade e utilização tecnológica do eucalipto (Eucalyptus globulus). Centro de Estudos Florestais, Lisbon
Pereira H (2013) Variability of the chemical composition of cork. BioResources 8:2246–2256
Kain G, Lienbacher B, Barbu M-C, Richter K, Petutschnigg A (2018) Larch (Larix decidua) bark insulation board: interactions of particle orientation, physical–mechanical and thermal properties. Euro J Wood Wood Prod 76:489–498
Maekawa E, Ichizawa T, Koshijima T (1989) An evaluation of the acid-soluble lignin determination in analyses of lignin by the sulfuric acid method. J Wood Chem Technol 9:549–567
Lourenço A, Pereira H (2017) Compositional variability of lignin in biomass. In: Poletto M (ed) Lignin-trends and applications. InTech, London, pp 65–98
Santos RB, Capanema EA, Balakshin MY, Chang H-m, Jameel H (2012) Lignin structural variation in hardwood species. J Agric Food Chem 60:4923–4930
Dou J, Kim H, Li Y, Padmakshan D, Yue F, Ralph J, Vuorinen T (2018) Structural characterization of lignins from willow bark and wood. J Agric Food Chem 66:7294–7300
Ralph J, Lapierre C, Boerjan W (2019) Lignin structure and its engineering. Curr Opin Biotechnol 56:240–249
Kawamoto H (2017) Lignin pyrolysis reactions. J Wood Sci 63:117–132
Rencoret J, Neiva D, Marques G, Gutiérrez A, Kim H, Gominho J, Pereira H, Ralph J, José C (2019) Hydroxystilbene glucosides are incorporated into Norway spruce bark lignin. Plant Physiol 180:1310–1321
Mottiar Y, Vanholme R, Boerjan W, Ralph J, Mansfield SD (2016) Designer lignins: harnessing the plasticity of lignification. Curr Opin Biotechnol 37:190–200
Van den Bosch S, Schutyser W, Vanholme R, Driessen T, Koelewijn SF, Renders T, De Meester B, Huijgen WJJ, Dehaen W, Courtin CM, Lagrain B, Boerjan W, Sels BF (2015) Reductive lignocellulose fractionation into soluble lignin-derived phenolic monomers and dimers and processable carbohydrate pulps. Energy Environ Sci 8:1748–1763
Ramos PA, Moreirinha C, Santos SA, Almeida A, Freire CS, Silva AM, Silvestre AJ (2019) Valorisation of bark lipophilic fractions from three Portuguese Salix species: a systematic study of the chemical composition and inhibitory activity on Escherichia coli. Ind Crop Prod 132:245–252
Devappa RK, Rakshit SK, Dekker RF (2015) Potential of poplar bark phytochemicals as value-added co-products from the wood and cellulosic bioethanol industry. BioEnergy Res 8:1235–1251
Ferreira JP, Miranda I, Sousa VB, Pereira H (2018) Chemical composition of barks from Quercus faginea trees and characterization of their lipophilic and polar extracts. PLoS One 13:e0197135
Willför S, Ali M, Karonen M, Reunanen M, Arfan M, Harlamow R (2009) Extractives in bark of different conifer species growing in Pakistan. Holzforschung 63:551–558
Hafizoğlu H, Holmbom B, Reunanen M (2002) Chemical composition of lipophilic and phenolic constituents of barks from Pinus nigra, Abies bornmülleriana and Castanea sativa. Holzforschung 56:257–260
Menezes JC, Edraki N, Kamat SP, Khoshneviszadeh M, Kayani Z, Mirzaei HH, Miri R, Erfani N, Nejati M, Cavaleiro JA (2017) Long chain alkyl esters of hydroxycinnamic acids as promising anticancer agents: selective induction of apoptosis in cancer cells. J Agric Food Chem 65:7228–7239
Freire C, Silvestre A, Neto CP, Cavaleiro J (2002) Lipophilic extractives of the inner and outer barks of Eucalyptus globulus. Holzforschung 56:372–379
Gominho J, Costa RA, Lourenço A, Quilhó T, Pereira H (2020) Eucalyptus globulus stumps bark: chemical and anatomical characterization under a valorisation perspective. Waste Biomass Valor:1–13
König M, Scholz E, Hartmann R, Lehmann W, Rimpler H (1994) Ellagitannins and complex tannins from Quercus petraea bark. J Nat Prod 57:1411–1415
Bianchi S, Kroslakova I, Janzon R, Mayer I, Saake B, Pichelin F (2015) Characterization of condensed tannins and carbohydrates in hot water bark extracts of European softwood species. Phytochemistry 120:53–61
Luís Â, Gil N, Amaral M, Duarte A (2012) Antioxidant activities of extracts from Acacia melanoxylon, Acacia dealbata and Olea europaea and alkaloids estimation. Int J Pharm Pharm Sci 4:225–231
Johansson A (1982) By-product recovery and valorisation in the kraft industry: a review of current trends in the recovery and use of turpentine and tall oil derivatives. Biomass 2:103–113
Scalbert A (1991) Antimicrobial properties of tannins. Phytochemistry 30:3875–3883
Funding
The Forest Research Center (CEF) was financed by Fundação para a Ciência e a Tecnologia (FCT), Portugal (UIDB/00239/2020). This work was performed in the framework of Catalisti-SBO project BIOWOOD and EoS project BIOFACT. T. V. acknowledges the Research Foundation Flanders (FWO Vlaanderen) for a doctoral fellowship and travel grant (1S64017N and V417219N). D. M. N. acknowledges a SUSFOR doctoral PhD scholarship from FCT (PD/BD/52697/2014). B. S. acknowledges EoS project BIOFACT and Catalisti-SBO project BIOWOOD for continuation of financial support for biorefinery research.
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Vangeel, T., Neiva, D.M., Quilhó, T. et al. Tree bark characterization envisioning an integrated use in a biorefinery. Biomass Conv. Bioref. 13, 2029–2043 (2023). https://doi.org/10.1007/s13399-021-01362-8
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DOI: https://doi.org/10.1007/s13399-021-01362-8