Abstract
Next generation sequencing was used to develop 15 new polymorphic microsatellite markers for the commercially and recreationally important fish mulloway (Argyrosomus japonicus). Improved knowledge of the genetic structure of the species is necessary for fisheries and environmental management. In a population of mulloway, we found that genetic variation and heterozygosity were high in most of our loci (mean number of alleles per locus = 9.46; mean heterozygosity = 0.660). To test the usefulness of our new markers for genetic research on other sciaenids, we cross amplified samples from eight other related sciaenid species, including three that form important fisheries and others that have significant conservation issues. We found that most of our new primers pairs produced scorable profiles in each of the other important sciaenids.
References
Archangi B, Chand V, Mather PB (2009) Isolation and characterization of 15 polymorphic microsatellite DNA loci from Argyrosomus japonicus (mulloway), a new aquaculture species in Australia. Mol Ecol Res 9(1):412–414
DeBoo ML, Bertozzi T, Donnellan SC, Mahony MJ (2012) Development of eight microsatellite loci from the Green and Golden Bell Frog (Litoria aurea) through GS-FLX pyrosequencing and cross-amplification with other species of the Litoria aurea species group. Conserv Genet Resour 4:1003–1005
Ferguson GJ, Ward TM, Geddes MC (2008) Do recent age structures and historical catches of mulloway, Argyrosomus japonicus (Sciaenidae), reflect freshwater inflows in the remnant estuary of the Murray River, South Australia? Aquat Living Resour 21(2):145–152
Gardner MG, Fitch AJ, Bertozzi T, Lowe AJ (2011) Rise of the machines–recommendations for ecologists when using next generation sequencing for microsatellite development. Mol Ecol Res 11(6):1093–1101
Hayden MJ, Nguyen T, Waterman A, McMichael G, Chalmers KJ (2008) Application of multiplex-ready PCR for fluorescence-based SSR genotyping in barley and wheat. Mol breed 21(3):271–281
Acknowledgments
Funding was provided by the Nature Foundation of South Australia Incorporated (scholarship grant to TCB and BMG-2010) and ARC Grants to BMG (DP110100716, FT100100767). We thank Mike Gardner, Alison Fitch and Leanne Payne for technical assistance, Anne-Marie Hegarty for supplying teraglin samples, Brad Pusey and Mark Kennard who collected some of the northern Australian croakers as part of research in Kakadu National Park and Susana Monsalve and Calogero Santoro for assisting with collection of lorna drum, and Ron Smernik for advice on this manuscript.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Barnes, T.C., Izzo, C., Bertozzi, T. et al. Development of 15 microsatellite loci from mulloway, Argyrosomus japonicus (Pisces: Sciaenidae) using next generation sequencing and an assessment of their cross amplification in other sciaenids. Conservation Genet Resour 6, 345–348 (2014). https://doi.org/10.1007/s12686-013-0090-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-013-0090-7