Abstract
Ethanol induces massive neuroapoptosis in the developing brain. However, whether ethanol-induced neuroapoptosis also involves GABA interneurons remains unknown. Here, we addressed this question in the postnatal days (P) P3-P24 GAD67-GFP mouse hippocampus using cleaved caspase-3 staining, a sensitive measure of ethanol-induced apoptotic neurodegeneration combined with DAPI staining to monitor the apoptotic nuclear degradation. We observed that 8 h following ethanol treatment (6 g/kg, intraperiotoneally), significant proportion of GAD67-GFP expressing hippocampal interneurons was stained with cleaved caspase-3 antibodies and displayed chromatin condensation with a formation of the DAPI-stained apoptotic bodies. Maximal number of the cleaved caspase-3 stained interneurons (16.6 % of the total number of GFP expressing neurons and 21.6 % of the total number of caspase-3 stained cells) was observed in the hippocampal slices from P6-9 mice, and minimal damage to interneurons was observed in P3–4 and > P11 mice. While the apoptotic interneurons were found in all hippocampal regions and layers, their highest density was observed in the CA1 region and hilus. Thus, ethanol-induced neuroapoptosis involves hippocampal interneurons that may contribute to the life-long neurobehavioral deficits, increased excitability, and higher incidence of seizures characteristic of the fetal alcohol spectrum disorders.
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Acknowledgments
This work was supported by INSERM (LIA to RK), the Program of Competitive Growth of Kazan Federal University, and the subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities.
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Elena Ogievetsky and Nailya Lotfullina contributed equally to this work.
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Ogievetsky, E., Lotfullina, N., Minlebaeva, A. et al. Ethanol-Induced Apoptosis of Interneurons in the Neonatal GAD67-GFP Mouse Hippocampus. BioNanoSci. 7, 151–154 (2017). https://doi.org/10.1007/s12668-016-0334-6
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DOI: https://doi.org/10.1007/s12668-016-0334-6