Abstract
Application of metallic nanoparticles (NPs) including silver (Ag), copper oxide (CuO), and zinc oxide (ZnO) has been individually investigated in a polyethylene matrix. Since the antibacterial agents have various mechanisms of action, a combinational system incorporating different NPs would be beneficial to increase the antibacterial activity through synergistic effect. Modeling the antibacterial action of low-density polyethylene films loaded with a combination of Ag, CuO, and ZnO was the main purpose of this study. A statistical mixture design approach was established to determine the effect of combinatorial use of NPs on the survival of Staphylococcus aureus and Escherichia coli. High R2 values (0.99 for S. aureus and 0.89 for E. coli) and non-significant (P > 0.05) lack of fit significantly (P < 0.05) fitted the proposed models to the experimental data. This ensured a satisfactory fitting of the regression models relating the independent variables to the response. A high negative effect of a combination of NPs on the growth of both bacteria was proven. At last, no significant (P < 0.05) differences were seen between the predicted and experimental responses for optimized nanocomposite composition validating the fitted models.
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Funding
Thanks to GAIN (Axencia Galega de Innovación) for supporting this review (grant number IN607A2019/01). Jose M. Lorenzo is member of the HealthyMeat network, funded by CYTED (ref. 119RT0568). B. Gullón would like to express her gratitude to the Spanish Ministry of Economy and Competitiveness for financial support (Grant reference RYC2018-026177-I).
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Samira Dehghani declares that she has no conflict of interest. Seyed Hadi Peighambardoust declares that he has no conflict of interest. Seyed Jamaleddin Peighambardoust declares that he has no conflict of interest. Seyedeh Homa Fasihnia declares that he has no conflict of interest. Nader Karimian Khosrowshahi declares that he has no conflict of interest. Beatriz Gullón declares that she has no conflict of interest. José M. Lorenzo declares that he has no conflict of interest.
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Dehghani, S., Peighambardoust, S.H., Peighambardoust, S.J. et al. Optimization of the Amount of ZnO, CuO, and Ag Nanoparticles on Antibacterial Properties of Low-Density Polyethylene (LDPE) Films Using the Response Surface Method. Food Anal. Methods 14, 98–107 (2021). https://doi.org/10.1007/s12161-020-01856-7
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DOI: https://doi.org/10.1007/s12161-020-01856-7