Abstract
Food industry is increasingly concerned in developing and applying rapid and nondestructive methods to offer safer and high quality foods to consumers. During the last years, Fourier transform near-infrared (FT-NIR) has been widely used to determine food quality based on spectrum. Likewise, FT-NIR has been proposed as an innovative and promising nondestructive rapid method capable to detect and identify microorganisms in foods; however, little progress has been made to date in this field. This study is a new attempt to apply FT-NIR technology to identify and quantify bacteria species in water-based systems in order to simulate water-based food matrices. For that, three different lactic acid bacteria—Lactobacillus plantarum, Leuconostoc mesenteroides, and Lactobacillus sakei—associated with spoilage in ready-to-eat meat, were analyzed by reflectance–transmitance FT-NIR in the spectral range 1,100–2,500 nm. Principal component analysis (PCA), and partial least squares (PLS) were applied to obtain prediction models. PCA and PLS showed a clear discrimination between the tested bacteria species whereas PLS method could succesfully quantify the concentration levels (3–9 log cfu/mL) and also distinguish between spoilage (7–9 log cfu/mL) and nonspoilage concentration levels (3–6 log cfu/mL). Results suggest that FT-NIR could be used efficiently to detect and quantify microorgasnisms in water-based food matrices. However, this study is an initial approach and therefore, it will be necessary to further research in order to really carry out its application to more complex food matrices and other microorganisms (i.e., food-borne pathogens).
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References
Alexandrakis D, Downey G, Scannelli AGM (2008) J Agric Food Chem 56(10):3431
Al-Qadiri HM, Lin M, Cavinato AG, Rasco BA (2006) Int J Food Microbiol 111:73–80
Arnoux AS, Preziosi-Belloy L, Esteban G, Teissier P, Ghommidh C (2005) Biotechnol Lett 27:1551
Ciurczak EW (1992) Principles a near-infrared spectroscopy. Handbook of near-infrared analysis. Dekker, New York
Duboys J, Neil Lewis E, Fry FS Jr, Calvey EM (2005) Food Microbiol 22:577
Goodacre R, Timmins EM, Burton R, Kaderbhai N, Woodward AM, Kell DB, Rooney PJ (1998) Microbiology 144:1157
Gupta MJ, Irudayaraj H, Debroy C (2004) J Food Protect 67(11):2250
Houtsma PC, De Wit JC, Rombouts FM (1993) Int J Food Microbiol 20:247
Jimenez-Marquez A, Molina-Diaz A, Pascual Reguera MI (2005) Sensor Actuat B-Chem 107:64
Lamber AD, Smith JP, Dobris KI (1991) Food Microbiol 8:267
Lima KMG, Raimundo IM Jr, Pimentel MF (2007) Sensor Actuat B-Chem 125:229–233
Lin M, Al-Holy M, Mousavi-Hesary M, Al-Qadiri A, Cavinato AG, Rasco Lett BA (2004) Appl Microbiol 39:148–155
McGlone VA, Kawano S (1998) Postharvest Biol Technol 13:131
Mendes LS, Oliveira FCC, Suarez PZ, Rubim JC (2003) Anal Chim Acta 493:219–231
Millar SP, Robert MF, Devaux F, Guy RCE, Marris P (1996) Appl Spectrosc 50:1134
Naes T, Isaksson T, Fearn T, Davies T (2004) A user-friendly guide to multivariate calibration and classification. NIR publications, Charlton, Chichester
Nicolaï BM, Beullens K, Bobelyn E, Peirs A, Saeys W, Theron KI, Lammertyn J (2007) Postharvest Biol Technol 44:99
Osborne BG, Fearn T, Hindle PH (1993) Practical NIR spectroscopy with applications in food and beverage analysis. Longman Scientific and Technical, Harlow
Polessello A, Giangicomo R (1981) Crit Rev Food Sci Nutr 18:203
Preisner O, Almeida Lopes J, Guiomar R, Machado JM, Menezes JC (2007) Anal Bioanal Chem 387:1739
Rodríguez-Saona LE, Khambaty FM, Fry FS, Calvey EM (2001) J Agr Food Chem 49:574
Rodríguez-Saona LE, Khambaty FM, Fry FS, Duboys J, Calvey EM (2004) J Food Protect 67:2555
Sarannwong S, Kawano S (2008) J Near Infrared Spectrosc 16:497–504
Sirisomboon P, Tanaka M, Fujita S, Kojima T (2007) J Food Eng 78:701
Toher D, Downey G, Murphy TB (2007) Chemometr Intell Lab Syst 89:102
Tripathi S, Mishra HN (2009) Food Control 20:840–846
Tsenkova R, Atanassova S, Morita H, Ikuta K, Toyoda K, Iordanova IK, Hakogi E (2006) J Near Infrared Spectrosc 14:363–370
Von Holy A, Cloete TE, Holzapfel WH (1991) Food Microbiol 8:95–104
Wang J, Sowa MG, Ahmed M, Mantsch HH (1994) J Phys Chem 98:4748
Wold S, Sjöström M, Eriksson L (2001) Chemometr Intell Lab Syst 58:109
Zannini E, Santarelli S, Osimani A, Dell’Aquila L, Clementi F (2005) Ann Microbiol 55:273–278
Acknowledgments
This work was partly financed by MICINN AGL2008-03298/ALI, the Excellence Project AGR-01879 (Junta de Andalucía) and by the Research Group AGR-170 HIBRO of the “Plan Andaluz de Investigación, Desarrollo e Innovación” (PAIDI).
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Cámara-Martos, F., Zurera-Cosano, G., Moreno-Rojas, R. et al. Identification and Quantification of Lactic Acid Bacteria in a Water-Based Matrix with Near-Infrared Spectroscopy and Multivariate Regression Modeling. Food Anal. Methods 5, 19–28 (2012). https://doi.org/10.1007/s12161-011-9221-5
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DOI: https://doi.org/10.1007/s12161-011-9221-5