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Hypoxia promotes the invasion and metastasis of laryngeal cancer cells via EMT

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Abstract

The purpose of this study is to explore the role of hypoxia on the invasion and metastasis of laryngeal carcinoma. The invasion and migration ability of laryngeal cancer SCC10A cell was detected by transwell assay. Western blot was applied to analyze the expression of EMT-related proteins. Fifty-seven samples from postoperative patients with laryngeal cancer were collected to study. Immunohistochemistry was used to examine the expression of GLUT-1 and EMT-related proteins (Vim, E-cad, N-cad) in normal laryngeal squamous epithelial tissue, laryngeal cancer adjacent tissues and laryngeal squamous cell carcinoma tissues. Hypoxia promoted laryngeal cancer cell invasion and migration. Hypoxia also enhanced the expression of GLUT-1, vimentin and N-cad, which exist statistically significant correlation with the clinical staging and lymph node metastases (P < 0.05). The expression of GLUT-1 is positively correlated with Vim and N-cad expression in laryngeal squamous cell carcinoma tissues, but negatively correlated with E-cad expression. The patient survival rate with the positive expression of GLUT-1, Vim and N-cad becomes much shorter compared with those with negative expression of GLUT-1, Vim and N-cad (P < 0.05). Hypoxia promoted laryngeal cancer cell invasion and migration via EMT.

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Acknowledgments

This work was supported by National Nature Science Foundation of China (81272960), Key Research Program from Science and Technology Department of Hunan Province, China (2013WK2010).

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    Authors and Affiliations

    1. Medical School, University of South China, Hengyang, 421001, Hunan, People’s Republic of China

      Jianhong Zuo, Juan Wen, Sai Li, Xiu Lv, Zhaoyang Luo & Gebo Wen

    2. Nanhua Hospital, University of South China, Hengyang, 421000, Hunan, People’s Republic of China

      Jianhong Zuo, Meiling Wen, Sai Li & Xiu Lv

    3. Department of Respiratory and Critical Care Medicine, Zhangjiajie City Hospital, Zhangjiajie, 427000, Hunan, People’s Republic of China

      Mingsheng Lei

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    1. Jianhong Zuo
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    Jianhong Zuo and Juan Wen have contributed equally to this work.

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    Zuo, J., Wen, J., Lei, M. et al. Hypoxia promotes the invasion and metastasis of laryngeal cancer cells via EMT. Med Oncol 33, 15 (2016). https://doi.org/10.1007/s12032-015-0716-6

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