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In vitro isolation and cultivation of human chondrocytes for osteoarthritis renovation

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Abstract

The purpose of this study was to evaluate the repair effects of chondrocytes that were cultured in vitro on osteoarthritis (OA). Chondrocytes were isolated from fetal rabbits and cultured in Biosilon microcarriers. Sixty rabbits were randomly divided into three groups equally (blank group, model group, treatment group). The rabbit knee OA model was established by inducing papain. Rabbits in the treatment group were injected with the chondrocytes that were cultured in vitro. Hematoxylin-eosin (HE) staining and gross morphologic observation were conducted. Expression level of cytokines such as IL-1bβ, IL-6, and TNF-α in cartilage synovial cells was also analyzed by an ELISA assay. The cultured chondrocyte was validated by a positive stain of type II collagen and vimentin by immunofluorescence. Compared to the model group, the articular cartilage of the rabbit knee in the treatment group showed a normal color, smooth surface, and none of malacia and coloboma. HE staining indicated that the articular surface of the treatment group tended to be smooth and flat; the matrix stained tinge and the cartilage destruction and fiber hyperplasia of the synovia were lightened. The expression levels of IL-1bβ, IL-6, and TNF-α also declined in the treatment group. OA symptoms were improved by treating with chondrocytes. In summary, the animal experiment in the present study indicated that chondrocyte injection played an active effect on renovation of OA.

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Acknowledgments

This work was supported by the Natural Science Foundation of China (no. C05030324).

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Authors and Affiliations

  1. Department of Orthopedics, The Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, 600 Yishan Road, 200233, Shanghai Province, China

    Jiaming Xu & Changqing Zhang

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  1. Jiaming Xu
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  2. Changqing Zhang
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Correspondence to Changqing Zhang.

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Editor: T. Okamoto

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Xu, J., Zhang, C. In vitro isolation and cultivation of human chondrocytes for osteoarthritis renovation. In Vitro Cell.Dev.Biol.-Animal 50, 623–629 (2014). https://doi.org/10.1007/s11626-014-9742-5

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  • DOI: https://doi.org/10.1007/s11626-014-9742-5

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