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Production of the Phanerochaete flavido-alba laccase in Aspergillus niger for synthetic dyes decolorization and biotransformation

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Abstract

We investigated the expression of Phanerochaete flavido-alba laccase gene in Aspergillus niger and the physical and biochemical properties of the recombinant enzyme (rLac-LPFA) in order to test it for synthetic dye biotransformation. A. niger was able to produce high levels of active recombinant enzyme (30 mgL−1), whose identity was further confirmed by immunodetection using Western blot analysis and N-terminal sequencing. Interestingly, rLac-LPFA exhibited an improved stability at pH (2–9) and organic solvents tested. Furthermore, the percentage of decoloration and biotransformation of synthetic textile dyes, Remazol Brilliant Blue R (RBBR) and Acid Red 299 (NY1), was higher than for the native enzyme. Its high production, simple purification, high activity, stability and ability to transform textile dyes make rLac-LPFA a good candidate for industrial applications.

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Acknowledgments

This work was partly funded by grant AGL2006-11697 from the Spanish Ministry of Economy and Competitiveness (formerly MICINN) and by the Spanish Ministry of Education and Science “Ayudas para la movilidad de estudiantes para la obtención de la mención europea en el título de doctor”.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Cartuja, 18071, Granada, Spain

    Lamiae Benghazi, José Martínez & Teresa de la Rubia

  2. INRA, Polytech Marseille, UMR 1163 de Biotechnologie des Champignons Filamenteux, Aix Marseille Université, CP 925, 13228, Marseille cedex 09, France

    Eric Record

  3. Department of Biochemistry and Molecular Biology 2, University of Granada, Campus Cartuja, 18071, Granada, Spain

    Antonio Suárez

  4. Department of Organic Chemical, Faculty of Pharmacy, University of Granada, Campus Cartuja, 18071, Granada, Spain

    José A. Gomez-Vidal

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  1. Lamiae Benghazi
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  2. Eric Record
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Correspondence to Teresa de la Rubia.

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Benghazi, L., Record, E., Suárez, A. et al. Production of the Phanerochaete flavido-alba laccase in Aspergillus niger for synthetic dyes decolorization and biotransformation. World J Microbiol Biotechnol 30, 201–211 (2014). https://doi.org/10.1007/s11274-013-1440-z

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