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Production of Extracellular Halo-alkaline Protease from a Newly Isolated Haloalkaliphilic Bacillus sp. Isolated from Seawater in Western India

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Summary

Haloalkaliphilic, gram positive, aerobic, coccoid Bacillus sp. Po2 was isolated from a seawater sample in Gujarat, India. On the basis of 16s rRNA gene homology, Po2 was 95% related to Bacillus pseudofirmus. A substantial level of extracellular alkaline protease was produced by Po2, which corresponded with the growth and reached a maximum level (264 U/ml) during the stationary phase at 24 h. The production thereafter remained nearly static at optimal level till 36 h. Po2 could grow in the range of 0–20% NaCl (w/v) and pH 7–9, optimally at 10% NaCl (w/v) and pH 8. The protease production was salt-dependent and optimum production required 15% NaCl (w/v) and pH 8. Among the organic nitrogen sources, optimum growth and protease production (260 U/ml) were supported by the combination of peptone and yeast extract. However, growth and protease production were highly suppressed by the inorganic nitrogen sources used; with the exception of potassium nitrate, which supported both growth and protease production to limited extent (24 U/ml). Strong inhibition of enzyme production was observed at above 1% glucose (w/v). Wheat flour served as both carbon and nitrogen source supporting growth and protease production.

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Acknowledgements

Financial Assistance from University Grants Commission (New Delhi, India) is acknowledged. The assistance provided by Dr B.K.C. Patel from Griffith University, Brisbane (Australia) for the 16S rRNA gene sequencing is also gratefully acknowledged. The authors are grateful to the Department of Biosciences and Saurashtra University for the necessary support.

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Authors and Affiliations

  1. Department of Life Sciences, University of North Gujarat, Patan, India

    R.K. Patel

  2. Department of Biosciences, Saurashtra University, 360 005, Rajkot, Gujarat, India

    M.S. Dodia, R.H. Joshi & S.P. Singh

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  1. R.K. Patel
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  2. M.S. Dodia
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  3. R.H. Joshi
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  4. S.P. Singh
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Correspondence to S.P. Singh.

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Patel, R., Dodia, M., Joshi, R. et al. Production of Extracellular Halo-alkaline Protease from a Newly Isolated Haloalkaliphilic Bacillus sp. Isolated from Seawater in Western India. World J Microbiol Biotechnol 22, 375–382 (2006). https://doi.org/10.1007/s11274-005-9044-x

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