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Cell wall compositional and vascular traits of hybrid poplar wood in micropropagated plants and plants propagated from root cuttings

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Abstract

The development of biotechnological approaches has increased the possibility to produce new genotypes of poplars with improved metabolic, growth, ecological or industrial properties. This study was aimed at determining whether or not propagation techniques affect cell wall composition and vascular traits in the wood of hybrid poplar. Clonally micropropagated plants and plants propagated from root cuttings were derived from mature hybrid poplar trees T-14 [Populus tremula L. × (Populus × canescens (Ait.) Sm.)] which were more than 30 years of age. Measurements were taken from both the vegetative plant origins following the seventh growing season after being planted in the field. These measurements included the content of main cell wall components and extractives, lignin monomer composition, neutral saccharide composition, macromolecular traits of both dioxane lignin and cellulose, and vascular traits. The micropropagated plants achieved significantly higher values for the content of cellulose, relative proportions of both d-glucose and d-mannose, and also for the weight-average molecular weight of dioxane lignin. The performance of the plants propagated from root cuttings was higher for traits primarily associated with the content of hemicelluloses, total yield of neutral saccharides, and for both absolute yields and relative proportions of d-xylose and l-arabinose. Similarities between both plant origins were found among the majority of the examined wood traits including the macromolecular traits of cellulose, lignin monomer composition and the vascular traits. Both plant origins formed compact homogeneous clusters clearly separated from each other in the multivariate wood trait analysis. There were no substantial drawbacks found in either the chemical profiles of the wood components or in the anatomical profiles of the vascular traits which could be attributed to the micropropagated plants of fast growing hybrid poplar. Seen from a practical viewpoint, hybrid poplar breeding programs could benefit from the compositional advantage of a higher cellulose content found in the micropropagated plants.

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Acknowledgements

The authors thank Mrs. E. Ritch-Krč for language revision. This work was supported by funding from the Slovak scientific grant agency VEGA (1/0450/19 and 1/0149/15).

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Authors and Affiliations

  1. Department of Phytology, Technical University in Zvolen, T.G. Masaryka 24, 960 01, Zvolen, Slovak Republic

    Jaroslav Ďurkovič, Hana Husárová & Ingrid Čaňová

  2. Department of Chemistry and Chemical Technologies, Technical University in Zvolen, T.G. Masaryka 24, 960 01, Zvolen, Slovak Republic

    František Kačík

  3. Department of Wood Processing, Czech University of Life Sciences in Prague, Kamýcká 1176, 165 00, Prague 6, Suchdol, Czech Republic

    František Kačík

  4. Department of Wood Science, Technical University in Zvolen, T.G. Masaryka 24, 960 01, Zvolen, Slovak Republic

    Miroslava Mamoňová

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  1. Jaroslav Ďurkovič
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Correspondence to Jaroslav Ďurkovič.

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Ďurkovič, J., Kačík, F., Husárová, H. et al. Cell wall compositional and vascular traits of hybrid poplar wood in micropropagated plants and plants propagated from root cuttings. New Forests 51, 119–135 (2020). https://doi.org/10.1007/s11056-019-09723-y

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