Abstract
Heat stress induces heat shock proteins (HSPs) expression and HSP70 family is one of them that have been reported to involve in cellular protection against heat stress. But whether there is any association of genetic variation in the HSP70A1A gene with thermo tolerance is unknown. PCR-SSCP and DNA sequencing were used to detect possible SNPs in HSP70A1A gene in 890 Chinese Holstein cattle. Three fragments were amplified and five novel mutations were found in HSP70A1A gene in Chinese Holstein cattle. G/A mutation was found at nucleotide 1524 in coding region that resulting two genotypes of AA and AB. T/C mutation was found at nucleotide 3494 in 3′-UTR resulting three genotypes of CC, CD and DD. The other three point mutations, G/C at nucleotide 6400, C/T at nucleotide 6600 and G/A at nucleotide 6601 were also found in 3′-UTR resulting six genotypes of EE, EF, FF, EG, FG and GG. Linkage disequilibrium (LD) analysis showed that the nucleotide 6400, 6600 and 6601 were in strong LD (D > 0.75). Association analysis indicated that AB, DD and FF genotype were the thermo tolerance genotype. SBYE Green I was used to quantify HSP70A1A mRNA expression in different tissues through quantitative real-time PCR assay. The results of the real-time PCR showed that the expression of HSP70A1A mRNA in the heart was significantly higher than that in the other tissues (P < 0.05). We presume that these mutations could be used in marker assisted selection for anti-heat stress cows in our breeding program.
Similar content being viewed by others
References
West JW (2003) Effects of heat-stress on production in dairy cattle. J Dairy Sci 86:2131–2144
Amaral BC, Connor EE, Tao S, Hayen J, Bubolz J, Dahl GE (2009) Heat-stress abatement during the dry period: does cooling improve transition into lactation? J Dairy Sci 92:5988–5999
Huang C, Tsuruta S, Bertrand JK, Misztal I, Lawlor TJ, Clay JS (2009) Trends for conception rate of Holsteins over time in the Southeastern United States. J Dairy Sci 92:4617–4641
Lee JA, Roussel JD, Beatty JF (1976) Effect of temperature-season on bovine adrenal cortical function, blood cell profile, and milk production. J Dairy Sci 59:104–108
Srikandakumar A, Johnson EH (2004) Effect of heat stress on milk production, rectal temperature, respiratory rate and blood chemistry in Holstein, Jersey and Australian Milking Zebu cows. Trop Anim Health Prod 36:685–692
Berman A (2005) Estimates of heat stress relief needs for Holstein dairy cows. J Anim Sci 83:1377–1384
Gaughan JB, Mader TL, Holt SM, Josey MJ, Rowan KJ (1999) Heat tolerance of Boran and Tuli crossbred steers. J Anim Sci 77:2398–2405
Evans JV (1954) Electrolyte concentrations in red blood cells of British breeds of sheep. Nature 174:931–932
Evans JV, Mounib M (1957) A survey of potassium concentration in the red blood cells of British breeds of sheep. J Agric Sci 48:433–437
Taneja GC, Abiehandani RK (1967) Evidence of genetic control of blood potassium type in the Marwari breed of sheep in India. Experientia 23:273–274
Christinaz P, Schatzmann HJ (1972) High potassium and low potassium erythrocytes in cattle. J Physiol 224:391–406
Soysal MI, Gurcan EK, Kök S (2005) A study of the distribution of potassium polymorphism in erythrocytes of grey cattle raised in the Edirne province of Turkey. Trakia J Sci 3:8–10
Shahrbabak HM, Shahrbabak MM, Yeganeh HM, Rahimi GH (2009) Association of the whole blood potassium polymorphism with other blood electrolytes in two sheep breeds of different climate of Iran. PWASET 37:2070–3740
Yang M, Tan H, Yang QL, Wang F, Yao HL, Wei QY, Tanguay RM, Wu TC (2006) Association of Hsp70 polymorphisms with risk of noise-induced hearing loss in Chinese automobile workers. Cell Stress Chaperones 11:233–239
Mosser DD, Caron AW, Bourget L, Meriin AB, Sherman MY, Morimoto RI, Massie B (2000) The chaperone function of Hsp70 is required for protection against stress-induced apoptosis. Mol Cell Biol 10:7146–7159
Xiao CF, Chen S, Yuan MC, Ding FY, Yang DL, Wang RB, Li JX, Tanguay RM, Wu TC (2004) Expression of the 60 kDa and 71 kDa heat shock proteins and presence of antibodies against the 71 kDa heat shock protein in pediatric patients with immune thrombocytopenic purpura. BMC Blood Disord 4:1–10
Bosch TC, Krylow SM, Bode HR, Steele RE (1988) Thermotolerance and synthesis of heat shock proteins: these responses are present in Hydra attenuata but absent in Hydra oligactis. Proc Natl Acad Sci USA 85:7927–7931
Sanchez Y, Parsell DA, Taulien J, Vogel JL, Craig EA, Lindquist S (1993) Genetic evidence for a functional relationship between Hsp104 and Hsp70. J Bacteriol 175:6484–6491
Maloyan A, Palmon A, Horowitz M (1999) Heat acclimation increases the basal HSP72 level and alters its production dynamics during heat stress. Am J Physiol Regul Integr Comp Physiol 276:R1506–R1515
Aufricht C (2005) Heat-shock protein 70: molecular supertool. Pediatr Nephrol 20:707–713
Sambrook J, Russell DW (2002) Molecular cloning a laboratory manual, 3rd edn. Translated by Huang Pei Tang. Science Press, Beijing
Yeh FC, Yang RC, Boyle T (1999) POPGENE Version 1.31, Microsoft window-based freeware for population genetic analysis. University of Alberta, Canada
Shi YY, He L (2005) SHEsis, a powerful software platform for analyses of linkage disequilibrium, haplotype construction, and genetic association at polymorphism loci. Cell Res 15:97–98
Li Z, Zhang Z, He Z, Tang W, Li T, Zeng Z, He L, Shi Y (2009) A partition-ligation-combination-subdivision EM algorithm for haplotype inference with multiallelic markers: update of the SHEsis. Cell Res 19:519–523
Zhang X, Du H, Li J (2002) Single nucleotide polymorphism of chicken heat shock protein (Hsp70) gene. In: 7th World congress on genetics applied to livestock production, France, Montpellier
Adamowicz T, Pers E, Lechniak D (2005) A New SNP in the 3′-UTR of the Hsp70–1 Gene in Bos taurus and Bos indicus. Biochem Genet 43:623–627
Liu Y, Li D, Li H, Zhou X, Wang G (2010) A novel SNP of the ATP1A1 gene is associated with heat tolerance traits in dairy cows. Mol Biol Rep (in press)
Leandro NS, Gonzales E, Ferro JA, Ferro MI, Givisiez PE, Macari M (2004) Expression of heat shock protein in broiler embryo tissues after acute cold or heat stress. Mol Reprod Dev 67:172–177
Acknowledgments
The study was supported by Ministry of Agriculture Project of China (nycytx-0107), 863 Project of China (2007AA10Z169, 2008AA101010-1), Grants of Well-bred Project from Shandong (2008LZ016-06A), Natural Science Foundation Project of Shandong (Y2008D56).
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Li, Q., Han, J., Du, F. et al. Novel SNPs in HSP70A1A gene and the association of polymorphisms with thermo tolerance traits and tissue specific expression in Chinese Holstein cattle. Mol Biol Rep 38, 2657–2663 (2011). https://doi.org/10.1007/s11033-010-0407-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11033-010-0407-5