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Adsorption of bovine serum albumin to a polymer brush prepared by atom-transfer radical polymerization in a porous inorganic membrane

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Abstract

Protein adsorption was performed by a polymer brush prepared by atom-transfer radical polymerization (ATRP) to a porous inorganic membrane. The porous inorganic membrane, Shirasu Porous Glass made from silica, was modified with a halogen-containing compound to bind the active species for the polymerization. Glycidyl methacrylate was polymerized from the halogen compound by ATRP for a prescribed time, and subsequently chemically modified. The progression of the chemical modification allowed the membrane to lower the phosphate-buffer flux of the porous membrane due to the attachment of the polymer brush. Bovine serum albumin (BSA), as a model protein, was adsorbed at 12 mg per gram of the membrane in permeating BSA solution through the polymer-brush-attached porous membrane.

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Acknowledgements

This work was supported in part by a Grant-in-Aid for Scientific Research (No.16760640) from the Ministry of Education, Culture, Sports, Science & Technology of Japan.

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Author notes

  1. Hidetaka Kawakita

    Present address: Department of Applied Chemistry, Saga University, Saga, 840-8502, Japan

Authors and Affiliations

  1. Advanced Research Institute for Science & Engineering, Waseda University, Kyushu, Kitakyushu, 808-0135, Japan

    Hidetaka Kawakita

  2. Kitakyushu Foundation for the Advancement of Industry Science and Technology, Kitakyushu, 808-0135, Japan

    Hiroyasu Masunaga & Kanako Nomura

  3. Department of Chemical Processes and Environments, Faculty of Environmental Engineering, The University of Kitakyushu, Kitakyushu, 808-0135, Japan

    Kazuya Uezu & Isamu Akiba

  4. Department of Chemical Engineering, Waseda University, 169-8555, Kitakyushu, Japan

    Satoshi Tsuneda

Authors
  1. Hidetaka Kawakita
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  2. Hiroyasu Masunaga
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  3. Kanako Nomura
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  4. Kazuya Uezu
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  5. Isamu Akiba
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  6. Satoshi Tsuneda
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Correspondence to Hidetaka Kawakita.

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Kawakita, H., Masunaga, H., Nomura, K. et al. Adsorption of bovine serum albumin to a polymer brush prepared by atom-transfer radical polymerization in a porous inorganic membrane. J Porous Mater 14, 387–391 (2007). https://doi.org/10.1007/s10934-006-9031-0

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  • DOI: https://doi.org/10.1007/s10934-006-9031-0

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