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A Novel Bacterial Polymeric Silk-Like Protein from a Petroleum Origin Bacillus sp. strain NE: Isolation and Characterization

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Abstract

This paper explores a novel silk-like protein (named Bacillus NE silk (BNES)) isolated from a petroleum origin bacterial strain belongs to the genus Bacillus. The successful characterizations for its chemical structure determination and protein composition have been performed. The results demonstrate that BNES protein is a new type of silk-like protein and has a prospective for applying as a new bacterial silk-like biomaterial. The amino acids constituents of silk-like protein showed that (Ala-Pro-Gly) rich-block protein, are the main major amino acids, while the protein Mwt., was recorded around > ~ 400 kDa, which excelled those similar of natural silk. The degummed or purified silk-like protein was successfully electro-spinned for wet-electrospun silk-like nanofibers using electrospinning technique. Silk-like nanofibers gave an adequate and uniform ribbon-shape structure with average diameter of nanofiber around 110 nm. Meanwhile, the block silk-like protein exhibited high thermal degradation monitored at 140–373 °C, which close those of natural silkworm silk. The purified silk-like protein showed no toxic effect against Vero kidney normal cell-line even after 48 h of incubation, using MTT-assay test. The obtained results demonstrate that, silk-like protein from wild type bacterial origin has potential for use as novel fibrous biomaterials for biomedical and environmental applications.

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References

  1. Jastrzebska K, Kucharczyka K, Florczak A, Dondajewska E, Mackiewicz A, Dams-Kozlowska H (2015) Silk as an innovative biomaterials for cancer therapy. Rep Pract Oncol Radiother 20:87–98

    Article  PubMed  Google Scholar 

  2. Hardy JG, Romer LM, Scheibel TR (2008) Polymeric materials based on silk proteins. Polymer 49:4309–4327

    Article  CAS  Google Scholar 

  3. Zhou CZ, Confalonieri F, Jacquet M, Perasso R, Li ZG, Janin J (2001) Silk fibroin structural implications of a remarkable amino acid sequence. Proteins 44(2):119–122

    Article  CAS  PubMed  Google Scholar 

  4. Anderson SO (1970) Amino acid composition of spider silks. Comp Biochem Physiol 35(3):705–711

    Article  Google Scholar 

  5. Slotta U, Hess S, Spiess K, Stromer T, Serpell L, Scheibel T (2007) Spider silk and amyloid fibrils: a structural comparison. Macromol Biosci 7:183–188

    Article  CAS  PubMed  Google Scholar 

  6. Spiess K, Lammel A, Scheibel T (2010) Recombinant spider silk proteins for applications in biomaterials. Macromol Biosci 10:998–1007

    Article  CAS  PubMed  Google Scholar 

  7. Fahnestock SR, Yao Z, Bedzyk LA (2000) Microbial production of spider silk proteins. Rev Mol Biotechnol 74:105–119

    Article  CAS  Google Scholar 

  8. Antony VAR, Chinnamal SK (2015) Enzymatic degumming of silk using Bacillus sp. Inter J Sci Tech Mang 4:458–465

    Google Scholar 

  9. Yang YJ, Choi YS, Jung D, Park BR, Hwang WB, Kim HW, Cha HJ (2013) Production of a novel silk-like protein from sea anemone and fabrication of wet-spun and electrospun marine-derived silk fibers. NPG Asia Mater 5:1–7

    Article  CAS  Google Scholar 

  10. Zaki S, Abu Elreesh G, Elkady M, Nosier M, Abd ElHaleem D (2011) Characterization of bioflocculants by bacteria isolated from cured petroleum oil. Int J Environ Sci Technol 8:831–840

    Article  CAS  Google Scholar 

  11. Murray E, Doetsch RN, Robinow CF (1994) Determinative and cytological light microscopy. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 21–41

    Google Scholar 

  12. Kim LS, Hong SJ, Son MK, Lee YH (2006) Polymeric and compositional properties of novel extracellular microbial polyglucosamine biopolymer from new strain of Citrobacter sp. BL-4. Biotechnol Lett 28:241–245

    Article  CAS  PubMed  Google Scholar 

  13. Liu W, Yuan H, Yang J, Li B (2009) Characterization of bioflocculants from biologically aerated filter backwashed sludge and its application in dying wastewater treatment. Bioresour Technol 100:2629–2632

    Article  CAS  PubMed  Google Scholar 

  14. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    CAS  PubMed  Google Scholar 

  15. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  CAS  PubMed  Google Scholar 

  16. El-Fakharany EM, Sánchez L, Al-Mehdar HA, Redwan EM (2013) Effectiveness of human, camel, bovine and sheep lactoferrin on the hepatitis C virus cellular infectivity: comparison study. Virol J 10(1):199

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63

    Article  CAS  PubMed  Google Scholar 

  18. Hardy JG, Romer LM, Scheibel TR (2008) Polymeric materials based on silk proteins. Polymer 49:4309–4327

    Article  CAS  Google Scholar 

  19. Babu KM (2012) Handbook of natural fibers: types, properties and factors affecting breeding and cultivation

  20. Nord LI, Vaag P, Duus J (2004) Quantification of organic and amino acids in beer by 1HNMR spectroscopy. Anal Chem 76:4790–4798

    Article  CAS  PubMed  Google Scholar 

  21. Wuethrich K (1986) NMR of proteins and nucleic acids. Wiley, New York, pp 11–13

    Google Scholar 

  22. Hu H, Kaplan DL, Cebe P (2006) Determining Beta-sheet crystallinity in fibrous proteins by thermal analysis and infrared spectroscopy. Macromolecules 39:6161–6170

    Article  CAS  Google Scholar 

  23. Boulet-Audet M, Vollrath F, Holland C (2015) Identification and classification of silks using infrared spectroscopy. J Exp Bio 2018:3138–3149

    Article  Google Scholar 

  24. Huang L, McMillan RA, Apkarian RP, Pourdeyhimi B, Conticello VP, Chaikof EL (2000) Generation of synthetic elastin-mimetic small diameter fibers and fiber networks. Macromolecules 33:2989

    Article  CAS  Google Scholar 

  25. Motta A, Fambri L, Migliaresi C (2002) Regenerated silk fibroin films: thermal and dynamic mechanical analysis. Macromol Chem Phys 203:1658–1665

    Article  CAS  Google Scholar 

  26. Kurosaki S, Otsuka H (1999) Fibroin allergy: IgE mediated hypersensitivity to silk suture materials. J Nippon Med Sch 66:41–44

    Article  CAS  Google Scholar 

  27. Agarwal N, Haogland DA, Farris RJJ (1997) Effect of moisture absorption on the thermal properties of Bombyx mori silk fibroin films. Appl Polym Sci 63:401–410

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Polymeric Materials Research Dep., Advanced Technology and New Materials Research Institute (ATNMRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg Al-Arab City, Alexandria, 21934, Egypt

    Elbadawy A. Kamoun

  2. Nanotechnology Research Center (NTRC), The British University in Egypt (BUE), El-Sherouk City, Cairo, 11837, Egypt

    Elbadawy A. Kamoun

  3. Environmental Biotechnology Research Dep., Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg Al-Arab City, Alexandria, 21934, Egypt

    Gadallah M. Abu-Elreesh & Desouky Abd-El-Haleem

  4. Therapeutic and Protective Proteins Laboratory, Proteins Research Dep., Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg Al-Arab City, Alexandria, 21934, Egypt

    Esmail M. El-Fakharany

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  1. Elbadawy A. Kamoun
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  2. Gadallah M. Abu-Elreesh
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  3. Esmail M. El-Fakharany
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  4. Desouky Abd-El-Haleem
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Correspondence to Elbadawy A. Kamoun or Desouky Abd-El-Haleem.

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Kamoun, E.A., Abu-Elreesh, G.M., El-Fakharany, E.M. et al. A Novel Bacterial Polymeric Silk-Like Protein from a Petroleum Origin Bacillus sp. strain NE: Isolation and Characterization. J Polym Environ 27, 1629–1641 (2019). https://doi.org/10.1007/s10924-019-01459-2

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  • DOI: https://doi.org/10.1007/s10924-019-01459-2

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