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Is (−)-Catechin a Novel Weapon of Spotted Knapweed (Centaurea stoebe)?

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Abstract

The novel weapons hypothesis states that some invasive weed species owe part of their success as invaders to allelopathy mediated by allelochemicals that are new to the native species. Presumably, no resistance has evolved among the native species to this new allelochemical (i.e., the novel weapon). In their native habitat, however, the plants that co-evolved with these invasive species have theoretically evolved defenses that obviate the allelochemical advantage. Previous studies have claimed that catechin is such a novel weapon of spotted knapweed (Centaurea stoebe = C. maculosa), an invasive species in the non-native habitat of North America. These studies indicated that (−)-catechin is more phytotoxic than (+)-catechin. Other studies have not found sufficient catechin in field soils to support this theory. We report that (−)-catechin and (+)-catechin are essentially equal, but poorly phytotoxic to a variety of plant species in bioassays without soil. In a dose/response experiment with Montana soils, we found the lowest dose for a growth reduction of two native Montana grasses (Koeleria macrantha and Festuca idahoensis) by a racemic mixture of (±)-catechin that ranged from about 25 to 50 mM, concentrations, orders of magnitude higher than expected in nature. Autoclaving the soil before adding the catechin did not affect the activity of catechin. We found (−)-catechin to be a potent antioxidant, in contrast to a previous claim that it acts as an allelochemical by causing oxidative stress. Our findings suggest that catechin is not a novel weapon of spotted knapweed and that other allelochemical(s) or alternative mechanisms must be found to explain the success of this species as an invader in North America.

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Acknowledgment

We thank C. Duncan for soil collections.

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Author notes

  1. Daniel Cook

    Present address: Poisonous Plant Research Laboratory, ARS, USDA, 1150 E. 14th N, Logan, UT, 84341, USA

  2. Amy C. Blair

    Present address: Department of Natural Resources, Fernow Hall, Cornell University, Ithaca, NY, 14853, USA

Authors and Affiliations

  1. NPURU, USDA, ARS, University, Oxford, MS, 38677, USA

    Stephen O. Duke, Franck E. Dayan, Robert D. Johnson, Kumudini M. Meepagala, Daniel Cook & Joanna Bajsa

  2. Department of Bioagricultural Sciences and Pest Management, Colorado State University, Ft. Collins, CO, 80523, USA

    Amy C. Blair

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  1. Stephen O. Duke
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Duke, S.O., Blair, A.C., Dayan, F.E. et al. Is (−)-Catechin a Novel Weapon of Spotted Knapweed (Centaurea stoebe)?. J Chem Ecol 35, 141–153 (2009). https://doi.org/10.1007/s10886-008-9587-z

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  • DOI: https://doi.org/10.1007/s10886-008-9587-z

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