Abstract
Little is known on antimicrobial peptide permeation through outer membrane channels in Gram-negative bacteria. Herein, we probed at a single-molecule level the interaction of two different peptides, magainin 2 and HPA3P with OmpF from E. coli. HPA3P is an analogue of the antimicrobial peptide HP(2–20) isolated from the N-terminal region of the Helicobacter pylori ribosomal protein. Our data show that the shorter and more charged HPA3P peptide is more accessible to the inner volume of the OmpF than magainin 2. We demonstrate the ability of HPA3P peptides to interact with OmpF in a voltage- and concentration-dependent manner, which does not rule out a novel mechanism by which such peptides could reach the periplasmic space of Gram-negative bacteria. Unexpectedly, we found that increasing the applied voltage led to an increase of the residence time of HPA3P peptide inside the pore, possibly reflecting electric field-induced changes in pore and peptide geometry.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aksimentiev A, Heng JB, Timp G, Schulten K (2004) Biophys J 87:2086–2097
Arzo MA, Celma JJG, Cervera J, Alcaraz A, Aguilella VM (2006) Bioelectrochemistry 71:22–29
Asandei A, Mereuta L, Luchian T (2008) Biophy Chem 135:32–40
Bessalle R, Kapitkovsky A, Gorea A, Shalit I, Fridkin M (1990) FEBS Lett 274:151–155
Bredin J, Simonet V, Iyer R, Delcour AH, Pages JM (2003) Biochem J 376:245–252
Brown KL, Hancock RE (2006) Curr Opin Immunol 18:24–30
Cascales E, Buchanan SK, Duche D, Kleanthous C, Lloubes R, Postle K, Riley M, Slatin S, Cavard D (2007) Microbiol Mol Biol Rev 71:158–229
Chan SC, Yau WL, Wang W, Smith DK, Sheu F-S, Chen HM (1998) J Pept Sci 4:413–425
Chiriac R, Luchian T (2007) Biophy Chem 130:139–147
Colquhoun D (1971) Lectures on biostatistics: an introduction to statistics with applications in biology and medicine. Clarendon, Oxford
Danelon C, Suenaga A, Winterhalter M, Yamato I (2003) Biophys Chem 104:591–603
Epand RM, Vogel HJ (1999) Biochim Biophys Acta 1462:11–28
Giangaspero A, Sandri L, Tossi A (2001) Eur J Biochem 268:5589–5600
Hancock REW, Lehrer R (1998) Trends Biotechnol 16:82–88
Heng JB, Ho C, Kim T, Timp R, Aksimentiev A, Grinkova YV, Sligar S, Schulten K, Timp G (2004) Biophys J 87:2905–2911
Hinnah SC, Wagner R, Sveshnikova N, Harrer R, Soll J (2002) Biophys J 83:899–911
Housden NG, Loftus SR, Moore GR, James R, Kleanthous C (2005) Proc. Natl Acad Sci USA 102:13849–13854
Kragol G, Lovas S, Varadi G, Condie BA, Hoffmann R, Otvos L Jr (2001) Biochemistry 40:3016–3026
Kullman L, Winterhalter M, Bezrukov SM (2002) Biophys J 82:803–812
Kurisu G, Zakharov SD, Zhalnina MV, Bano S, Eroukova VY, Rokitskaya TI, Antonenko YN, Wiener MC, Cramer WA (2003) Nat Struct Biol 10:948–954
Law CJ, Penfold CN, Walker DC, Moore GR, James R, Kleanthous C (2003) FEBS Lett 545:127–132
Lee KH, Lee DG, Park Y, Kang D-I, Shin SY, Hahm K-S, Kim Y (2006) Biochem J 394:105–114
Lehrer RI, Barton A, Daher KA, Harwig SSL, Ganz T, Selsted ME (1989) J Clin Invest 84:553–561
Li J, Gersho M, Stein D, Brandin E, Aziz MJ, Golovchenko JA (2003) Nat Mater 2:611–615
Lindemann M, Winterhalter M (2006) IEE Proc Nanobiotechnol 153:107–111
Luchian T, Shin S-H, Bayley H (2003) Angew Chem Int Ed 42:1925–1929
McPhee JB, Hancock RE (2005) J Pept Sci 11:677–687
Mereuta L, Luchian T, Park Y, Hahm K-S (2008) Biochem Biophys Res Commun 373:467–472
Mereuta L, Luchian T, Park Y, Hahm K-S (2009) J Bioenerg Biomem 41:79–84
Merrifield RB, Juvvadi P, Andreu D, Ubach J, Boman A, Boman HG (1995) Proc Natl Acad Sci U S A 92:3449–3453
Movileanu L, Schmittschmitt JP, Scholtz JM, Bayley H (2005) Biophys J 89:1030–1045
Nestorovich EM, Danelon C, Winterhalter M, Bezrukov SM (2002) Proc. Natl Acad Sci USA 99(15):9789–9794
Nestorovich EM, Rostovtseva TK, Bezrukov SM (2003) Biophys J 85:1–12
Nikaido H (2003) Microbiol Mol Biol Rev 67:593–656
Oren Z, Shai Y (1996) Eur J Biochem 237:303–310
Park CB, Kim HS, Kim SC (1998) Biochem Biophys Res Commun 244:253–257
Park SC, Kim MH, Hossain MA, Shin SY, Kim Y, Stella L, Wade JD, Park Y, Hahm K-S (2008) Biochim Biophys Acta 1788:229–241
Seydel U, Eberstein W, Schroder G, Brandenburg KZ (1992) Naturforsch 47c:757–761
Schirmer T (1998) J Struct Biol 121:101–109
Sharma O, Cramer WA (2007) J Bacteriol 189:363–368
Shimoda M, Ohki K, Shimamoto Y, Kohashi O (1995) Infect Immun 63:2886–2891
Stefureac R, Long Y-T, Kraatz HB, Howard P, Lee JS (2006) Biochemistry 45:9172–9179
Subbalakshmi C, Sitaram N (1998) FEMS Microbiol Lett 160:91–96
Sutherland TC, Long Y-T, Stefureac RI, Bediako-Amoa I, Kraatz HB, Lee JS (2004) Nanoletters 4:1273–1277
Tossi A (2005) Curr Protein Pept Sci 6:1–3
Wade D, Boman A, Wahlin B, Drain CM, Andreu D, Boman HG, Merrifield RB (1990) Proc Natl Acad Sci U S A 87:4761–4765
Wiese A, Reiner JO, Brandenburg K, Kawahara K, Zahringer U, Seydel U (1996) Biophys J 70:321–329
Wolfe AJ, Mohammad MM, Cheley S, Bayley H, Movileanu L (2007) J Am Chem Soc 129:14034–14041
Yamashita E, Zhalnina MV, Zakharov SD, Sharma O, Cramer WA (2008) EMBO J 27:2171–2180
Yang L, Harroun TA, Weiss TM, Ding L, Huang HW (2001) Biophys J 81:1475–1485
Zakharov SD, Eroukova VY, Rokitskaya TI, Zhalnina MV, Sharma O, Loll PJ, Zgurskaya HI, Antonenko YN, Cramer WA (2004) Biophys J 87:3901–3911
Zasloff M (2002) Nature 415:389–395
Zelezetsky I, Pacor S, Pag U, Papo N, Shai Y, Sahl HG, Tossi A (2005) Biochem J 390:177–188
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Apetrei, A., Asandei, A., Park, Y. et al. Unimolecular study of the interaction between the outer membrane protein OmpF from E. coli and an analogue of the HP(2–20) antimicrobial peptide. J Bioenerg Biomembr 42, 173–180 (2010). https://doi.org/10.1007/s10863-010-9273-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10863-010-9273-z