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Osteogenic differentiation of mesenchymal progenitor cells in computer designed fibrin-polymer-ceramic scaffolds manufactured by fused deposition modeling

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Abstract

Biomimetic scaffolds offer great potentials in the development of bone analogs for tissue engineering. The studies presented in this paper focus specifically on the osteogenic potential of the novel PCL/CaP matrices and its degradation behavior. Biodegradable Polymer-ceramic Scaffolds were fabricated using the solid free form fabrication technology: Fused Deposition Modeling (FDM). The scaffold architecture was characterized by a honeycomb-like design and a complete interconnectivity of the pores. Human mesenchymal stem cells (MSCs) were seeded together with fibrin glue into PCL/CaP scaffolds and cultured in vitro for periods of up to eight weeks. Cellular adhesion, proliferation and osteogenic differentiation were assessed in these constructs using a range of histological and microscopic techniques. In additional experiments, degradation was assessed by measuring mass loss, diameter change, molecular weight change and by scanning electron micrographs. MSCs were able to adhere, migrate, and differentiate along the osteogenic lineage with in these scaffolds. The PCL/CaP scaffolds showed up to 27 fold increased degradation of compared to PCL scaffolds.

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Authors and Affiliations

  1. Division of Bioengineering, National University of Singapore, Singapore

    Jan-Thorsten Schantz, Dietmar Werner Hutmacher & Hwei Ling Khor

  2. Division of Plastic Surgery, National University of Singapore, Singapore

    Jan-Thorsten Schantz

  3. Department of Orthopedic Surgery, National University of Singapore, Singapore

    Dietmar Werner Hutmacher

  4. Department of Material Science, National University of Singapore, Singapore

    Hwei Ling Khor

  5. Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia

    Arthur Brandwood

  6. Baxter Biosurgery, Munich, Germany

    Katharina Bittner

Authors
  1. Jan-Thorsten Schantz
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  2. Arthur Brandwood
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  3. Dietmar Werner Hutmacher
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  4. Hwei Ling Khor
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  5. Katharina Bittner
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Correspondence to Jan-Thorsten Schantz.

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Schantz, JT., Brandwood, A., Hutmacher, D.W. et al. Osteogenic differentiation of mesenchymal progenitor cells in computer designed fibrin-polymer-ceramic scaffolds manufactured by fused deposition modeling. J Mater Sci: Mater Med 16, 807–819 (2005). https://doi.org/10.1007/s10856-005-3584-3

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  • DOI: https://doi.org/10.1007/s10856-005-3584-3

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