Abstract
Purpose
Polycystic ovary syndrome (PCOS) is an endocrine-metabolic disorder that leads to lower natural reproductive potential and presents a challenge for assisted reproductive medicine because patients may exhibit immature oocyte retrieval and a higher risk of ovarian hyper stimulation syndrome during in vitro fertilization (IVF) treatment. This study aimed to identify potential lipid biomarkers for women with PCOS and a hyper response to controlled ovarian stimulation.
Methods
Follicular fluid samples were collected from patients who underwent IVF, including normal responder women who became pregnant (control group, n = 11), women with PCOS and a hyper response to gonadotropins (PCOS group, n = 7) and women with only hyper response to gonadotropins (HR group, n = 7). A lipidomic analysis was performed by electrospray ionization mass spectrometry, and candidate biomarkers were analyzed by tandem mass spectrometry experiment.
Results
The lipid profiles indicated particularities related to differences in phosphatidylcholine (PCOS and HR), phosphatidylserine, phosphatydilinositol and phosphatidylglycerol (control), sphingolipids (PCOS) and phosphatidylethanolamine (control and HR).
Conclusions
These findings contribute to the understanding of the molecular mechanisms associated with lipid metabolism in the PCOS-related hyper response, and strongly suggest that these lipids may be useful as biomarkers, leading to the development of more individualized treatment for pregnancy outcome.
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Acknowledgments
This work was supported by CNPq (National Council for Scientific and Technological Development), Brazil and by grant 2012/06389-4, São Paulo Research Foundation (FAPESP).
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All authors declare that there are no conflicts of interest.
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Capsule Follicular fluid lipid profile by ESI/MS.
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Cordeiro, F.B., Cataldi, T.R., do Vale Teixeira da Costa, L. et al. Follicular fluid lipid fingerprinting from women with PCOS and hyper response during IVF treatment. J Assist Reprod Genet 32, 45–54 (2015). https://doi.org/10.1007/s10815-014-0375-0
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DOI: https://doi.org/10.1007/s10815-014-0375-0