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Prediction of oocyte developmental competence in ovine using glucose-6-phosphate dehydrogenase (G6PDH) activity determined at retrieval time

  • Gamete Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To determine whether G6PDH-activity measured by Brilliant Cresyl Blue known as BCB dye, predicts developmental competence within cohorts of ovine oocytes.

Methods

Ovine oocytes were exposed to BCB staining and categorized into two groups: BCB+ (blue cytoplasm, low G6PDH-activity) and BCB- (colorless cytoplasm, high G6PDH-activity). After maturation in vitro, oocytes were subjected to fertilization followed by in vitro embryo culture.

Results

We observed a significant difference in oocyte diameter considering BCB+ and BCB- oocytes. BCB+ and Control groups showed significantly higher maturation rates compared to BCB- group. There were significantly more cleaved embryos in BCB+ and control groups than in BCB- group. Blastocyst rate was significantly higher for BCB+ group compared to control and BCB- groups with control group being significantly higher than BCB- group.

Conclusion

G6PDH-activity is a strong predictive marker of oocyte competence and may be useful in identifying oocytes with a good prognosis for further develop.

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Acknowledgments

The authors thank the members of their own laboratories for their helpful discussions. We are also indebted to Miss Lida Langroudi for editing the manuscript.

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Authors and Affiliations

  1. Department of Transgenic Animal Science, Stem Cell Technology Research Center, Tehran, Iran

    Abdollah Mohammadi-Sangcheshmeh, Masoud Soleimani, Mohammad Salehi, Sara Soudi & Seyed Mahmoud Hashemi

  2. Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Endenicher Allee 15, Bonn, Germany

    Abdollah Mohammadi-Sangcheshmeh, Karl Schellander & Michael Hoelker

  3. Saint Mary Fertility Center, Nimeh Shaban Hospital, Sari, Iran

    Abdollah Mohammadi-Sangcheshmeh

  4. Department of Hematology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran

    Masoud Soleimani

  5. Department of Animal Science, Sari Agricultural Sciences & Natural Resources University, Sari, Iran

    Hamid Deldar

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  1. Abdollah Mohammadi-Sangcheshmeh
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  2. Masoud Soleimani
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  3. Hamid Deldar
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  4. Mohammad Salehi
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  7. Karl Schellander
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  8. Michael Hoelker
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Correspondence to Abdollah Mohammadi-Sangcheshmeh.

Additional information

Capsule Glucose-6-phosphate dehydrogenase activity in ovine oocytes prior to in vitro maturation is associated with oocyte diameter, meiotic competence and developmental capacity to the blastocyst stage in vitro.

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Mohammadi-Sangcheshmeh, A., Soleimani, M., Deldar, H. et al. Prediction of oocyte developmental competence in ovine using glucose-6-phosphate dehydrogenase (G6PDH) activity determined at retrieval time. J Assist Reprod Genet 29, 153–158 (2012). https://doi.org/10.1007/s10815-011-9625-6

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  • DOI: https://doi.org/10.1007/s10815-011-9625-6

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