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Expression of miRNA-155, miRNA-223, miRNA-31, miRNA-21, miRNA-125b, and miRNA-146a in the Inflammatory Pathway of Hidradenitis Suppurativa

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Abstract

Hidradenitis suppurativa (HS) has been associated with marked inflammatory perturbation. The mechanisms regulating the inflammatory network remain elusive. microRNAs (miRNAs) have been described as gene regulators of inflammation. We evaluated the messenger RNA (mRNA) expression levels of six selected inflammation-related miRNAs in lesional and perilesional skin samples of HS patients and in healthy controls. Samples of 15 HS patients and 10 healthy controls were included in this prospective study. Expression levels of the miRNAs miRNA-155-5p, miRNA-223-5p, miRNA-31-5p, miRNA-21-5p, miRNA-125b-5p, and miRNA-146a-5p were studied by quantitative real-time reverse transcription polymerase chain reaction. We observed a significant overexpression of miRNA-155-5p, miRNA-223-5p, miRNA-31-5p, miRNA-21-5p, and miRNA-146a-5p in lesional HS skin compared to healthy controls. Expression of these miRNAs was also significantly increased in lesional HS skin when compared to perilesional skin. Only miRNA-155-5p showed an increased expression in perilesional skin compared to healthy controls. In contrast, miRNA-125b-5p had a significantly lower expression in lesional HS skin compared to perilesional skin. We found that the studied inflammation-related miRNAs were significantly dysregulated in lesional HS skin and may have regulatory roles in the inflammatory process of HS. Given their predicted targets and functions, our findings point to these miRNAs as potential disease biomarkers, and manipulation might be used therapeutically to target the inflammatory pathway in HS.

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Abbreviations

HS:

Hidradenitis suppurativa

miRNA:

microRNA

mRNA:

Messenger RNA

TNF-α:

Tumor necrosis factor-α

IL:

Interleukin

qRT-PCR:

Quantitative real-time reverse transcription polymerase chain reaction

SHIP-1:

Src homology 2-containing inositol phosphatase-1

TLR:

Toll-like receptor

TH:

T helper

FGFR2:

Fibroblast growth factor receptor 2

LPS:

Lipopolysaccharide

IRAK-1:

IL-1 receptor-associated kinase 1

TRAF-6:

TNF receptor-associated factor 6

STK40:

Serine/threonine kinase 40

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Authors and Affiliations

  1. Dermatologic Surgery Department, Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum, Gudrunstr. 56, 44791, Bochum, Germany

    S. Hessam, M. Sand, M. Skrygan, T. Gambichler & F. G. Bechara

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  1. S. Hessam
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  2. M. Sand
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  3. M. Skrygan
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Correspondence to F. G. Bechara.

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This study was approved by the Ethical Review Board of the Ruhr-University Bochum, Germany (registration no.: 5076–14).

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki Declaration and its later amendments.

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Hessam, S., Sand, M., Skrygan, M. et al. Expression of miRNA-155, miRNA-223, miRNA-31, miRNA-21, miRNA-125b, and miRNA-146a in the Inflammatory Pathway of Hidradenitis Suppurativa. Inflammation 40, 464–472 (2017). https://doi.org/10.1007/s10753-016-0492-2

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