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Establishment and characterization of a heart-derived cell line from goldfish (Carassius auratus)

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Abstract

The goldfish Carassius auratus, a freshwater fish in the family Cyprinidae, was one of the earliest fish to be domesticated for ornamental purposes. A cell line was established from goldfish heart (GH) tissue to create a biological monitoring tool for viral diseases. The GH cell line was optimally maintained at 25 °C in M199 medium supplemented with 10–20% fetal bovine serum. A chromosomal analysis indicated that the cell line remained diploid, with a mean chromosomal count of 100. In viral inoculation assays, significant cytopathic effects (CPEs) were caused by epizootic hematopoietic necrosis virus (EHNV), Andrias davidianus iridovirus (ADIV), and Bohle iridovirus (BIV) infections in the fish cells and the viral titers (average value) of EHNV, ADIV, and BIV in GH cells reached 105.0, 104.5, and 105.0 TCID50/0.1 mL, respectively, within 7 days. However, no CPE was observed in the cells infected with viral hemorrhagic septicemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV), spring viremia of carp virus (SVCV), infectious pancreatic necrosis virus (IPNV), channel catfish virus (CCV), or grass carp reovirus (GCRV). These results suggest that the GH cell line is a valuable tool for studying viral pathogenesis.

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Acknowledgements

The work was supported by the National Key Technology R&D Program (No. 2013BAD12B02) in China.

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Authors and Affiliations

  1. Chinese Academy of Inspection and Quarantine, Beijing, 100029, China

    Hongli Jing, Xiangmei Lin, Min Zhang, Na Wang & Shaoqiang Wu

  2. Beijing Aquaculture Technology Extension Station, Beijing, 100021, China

    Lipu Xu

  3. She kou in shen zhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518067, China

    Longying Gao

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  1. Hongli Jing
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  2. Xiangmei Lin
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  3. Lipu Xu
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  4. Longying Gao
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  5. Min Zhang
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  6. Na Wang
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  7. Shaoqiang Wu
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Correspondence to Shaoqiang Wu.

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Jing, H., Lin, X., Xu, L. et al. Establishment and characterization of a heart-derived cell line from goldfish (Carassius auratus). Fish Physiol Biochem 43, 977–986 (2017). https://doi.org/10.1007/s10695-017-0345-4

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  • DOI: https://doi.org/10.1007/s10695-017-0345-4

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