Abstract
We analysed family relationships among brown trout from two small tributary populations that have been suggested as a source of individuals for supportive breeding, using variation at eight microsatellite loci. As a control, we analysed a sample of supposedly unrelated individuals representing a large anadromous population, and we simulated unrelated individuals based on the allelic distributions in all three samples. Two different approaches were used: (1) pairwise estimates of relatedness between individuals and (2) a method for partitioning individuals into half-sib and full-sib families. The anadromous population did not show evidence of a significant number of closely related individuals. In both tributary populations, however, the distributions of pairwise relatedness estimates suggested the presence of several related individuals, and sibship reconstruction suggested fewer families consisting of more individuals than were observed for the simulated individuals. The expected increase of inbreeding coefficient in the two samples due to family structure was 0.026 and 0.030 respectively. Moreover, tests for recent bottlenecks yielded significant outcomes in both populations suggesting a history of low effective population sizes. Depending on the effective population size of captive spawners and past effective population sizes in the populations it could be beneficial to conduct sib-avoidance matings, though this cannot eliminate inbreeding but only delay it. Alternatively, individuals from different populations could be crossed. Sibship reconstruction provided the clearest evidence for family structure, but pairwise relatedness is the best measure for designing mating schemes, as it allows for mating as unrelated individuals as possible rather than just avoiding mating between sibs.
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References
MS Blouin (2003) ArticleTitleDNA-based methods for pedigree reconstruction and kinship analysis in natural populations Trends Ecol. Evol., 18 503–511
MS Blouin M Parsons V Lacaille S Lotz (1996) ArticleTitleUse of microsatellite loci to classify individuals by relatedness Mol. Ecol., 5 393–401
J Carlsson JEL Carlsson KH Olsén MM Hansen T Eriksson J Nilsson (2004) ArticleTitleKin-biased distribution in brown trout: an effect of redd location or kin recognition? Heredity, 92 53–60
A Di Rienzo AC Peterson JC Garza AM Valdès M Slatkin NB Freimer (1994) ArticleTitleMutational processes of simple-sequence repeat loci in human populations Proc. Natl Acad. Sci. USA, 91 3166–3170
RW Doyle R Perez-Enriquez K Takagi N Taniguchi (2001) ArticleTitleSelective recovery of founder genetic diversity in aquacultural broodstocks and captive, endangered fish populations Genetica, 111 291–304
P Duchesne L Bernatchez (2002) ArticleTitleAn analytical investigation of the dynamics of inbreeding in multi-generation supportive breeding Conserv. Genet., 3 45–58
JM Elliott (1994) Quantitative Ecology and the Brown Trout Oxford University Press Oxford, UK 286
A El Mousadik RJ Petit (1996) ArticleTitleHigh level of genetic differentiation for allelic richness among populations of the argan tree [Argania spinosa (L.) Skeels] endemic to Morocco Theor. Appl. Genet., 92 832–839
A Estoup P Presa F Krieg D Vaiman R Guyomard (1993) ArticleTitle(CT)n and (GT)n microsatellites: a new class of genetic markers for Salmo trutta L. (brown trout) Heredity, 71 488–496
A Estoup CR Largiader E Perrot D Chourrout (1996) ArticleTitleRapid one-tube DNA extraction for reliable PCR detection of fish polymorphic markers and transgenes Mol. Mar. Biol. Biotechnol., 5 295–298
A Estoup F Rousset Y Michalakis JM Cornuet M Adriamanga R Guyomard (1998) ArticleTitleComparative analysis of microsatellite and allozyme markers: a case study investigating microgeographic differentiation in brown trout (Salmo trutta) Mol. Ecol., 7 339–353
DS Falconer (1989) Introduction to Quantitative Genetics Longman Scientific and Technical Essex, UK
JC Garza EG Willamson (2001) ArticleTitleDetection of reduction in population size using data from microsatellite loci Mol. Ecol., 10 305–318
J Goudet (1995) ArticleTitleFSTAT (version 1.2): a computer program to calculate F-statistics J. Hered., 86 485–486
SW Guo EA Thompson (1992) ArticleTitlePerforming the exact test for Hardy–Weinberg proportion for multiple alleles Biometrics, 48 361–372
MM Hansen (2002) ArticleTitleEstimating the long-term effects of stocking domesticated trout into wild brown trout (Salmo trutta) populations: an approach using microsatellite DNA analysis of historical and contemporary samples Mol. Ecol., 11 1003–1015
MM Hansen EE Nielsen KLD Mensberg (1997) ArticleTitleThe problem of sampling families rather than populations: relatedness among individuals in samples of juvenile brown trout (Salmo trutta L.). Mol Ecol., 6 469–474
MM Hansen DE Ruzzante EE Nielsen D Bekkevold K-LD Mensberg (2002) ArticleTitleLong-term effective population sizes, temporal stability of genetic composition and potential for local adaptation in anadromous brown trout (Salmo trutta) populations Mol. Ecol., 11 2523–2535
OJ Hardy X Vekemans (2002) ArticleTitleSPAGeDI : a versatile computer program to analyse spatial genetic structure at the individual or population levels Mol. Ecol. Notes, 2 618–620
EE Nielsen MM Hansen V Loeschcke (1999) ArticleTitleAnalysis of DNA from old scale samples: technical aspects, applications and perspectives for conservation Hereditas, 130 265–276
EE Nielsen MM Hansen L Bach (2001) ArticleTitleLooking for a needle in a haystack: discovery of indigenous salmon in heavily stocked populations Conserv. Genet., 2 219–232
PT O’Reilly LC Hamilton SK McConnell JM Wright (1996) ArticleTitleRapid analysis of genetic variation in Atlantic salmon (Salmo salar) by PCR multiplexing of dinucleotide and tetranucleotide microsatellites Can. J. Fish. Aquat. Sci., 53 2292–2298
D Paetkau W Calvert I Stirling C Strobeck (1995) ArticleTitleMicrosatellite analysis of population structure in Canadian polar bears Mol. Ecol., 4 347–354
DC Queller KF Goodnight (1989) ArticleTitleEstimating relatedness using genetic markers Evolution, 43 258–275
M Raymond F Rousset (1995) ArticleTitleGENEPOP (version 1.2): a population genetics software for exact tests and ecumenicism J. Hered., 86 248–249
WR Rice (1989) ArticleTitleAnalyzing tables of statistical tests Evolution, 43 223–225
N Ryman L Laikre (1991) ArticleTitleEffects of supportive breeding on the genetically effective population size Cons. Biol., 5 325–329
A Slettan I Olsaker Ø Lie (1995) ArticleTitleAtlantic salmon, Salmo salar, microsatellites at the SSOSL25, SSOSL85, SSOSL311, SSOSL417 loci Anim. Genet., 26 277–285
A Slettan I Olsaker Ø Lie (1996) ArticleTitleAtlantic salmon, Salmo salar, microsatellites at the SSOSL438, SSOSL439 and SSOSL444 loci Anim. Genet., 27 57–64
DA Tallmon G Luikart RS Waples (2004) ArticleTitleThe alluring simplicity and complex reality of genetic rescue Trends. Ecol. Evol., 19 489–496
JL Wang (2004) ArticleTitleSibship reconstruction from genetic data with typing errors Genetics, 166 1963–1979
J Wang N Ryman (2001) ArticleTitleGenetic effects of multiple generations of supportive breeding Cons. Biol., 15 1619–1631
RS Waples C Do (1994) ArticleTitleGenetic risk associated with supplementation of Pacific salmonids: captive broodstock programs Can. J. Fish. Aquat. Sci., 51 IssueIDSuppl. 1 310–329
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Hansen, M.M., Jensen, L.F. Sibship within samples of brown trout (Salmo trutta) and implications for supportive breeding. Conserv Genet 6, 297–305 (2005). https://doi.org/10.1007/s10592-004-7827-5
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DOI: https://doi.org/10.1007/s10592-004-7827-5