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Improvement of platelet aggregation and rapid induction of hemostasis in chitosan dressing using silver nanoparticles

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Abstract

There is an increasing demand for high-performance hemostatic agents with good antibacterial efficiency and biocompatibility. In this study, a chitosan/gelatin/sodium hyaluronate hemostatic dressing with silver nanoparticles (CGSH/Ag50) was designed and characterized. Our results suggest that CGSH/Ag50 has high blood absorption and promotes platelet aggregation by the activation of positively charged surface and stimulation of silver nanoparticles. Owing to these excellent properties, CGSH/Ag50 could rapidly control hemorrhage in rabbit ear and liver injury models. CGSH/Ag50 also shows good antibacterial activity, good liquid absorption, and significantly promotes full-thickness wound healing. In the hemolysis and L929 cell cytotoxicity assays, CGSH/Ag50 exhibited no cytotoxicity, suggesting good hemocompatibility and biocompatibility. These observations suggest that CGSH/Ag50 can be an efficient hemostatic material with enough biocompatibility to be used for controlling hemorrhage and promoting wound healing after hemostasis surgery.

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References

  • Abdel-Rahman RM, Abdel-Mohsen AM, Hrdina R, Burgert L, Fohlerova Z, Pavliňák D, Sayed ON, Jancar J (2016) Wound dressing based on chitosan/hyaluronan/nonwoven fabrics: preparation, characterization and medical applications. Int J Biol Macromol 89:725–736

    CAS  PubMed  Google Scholar 

  • Baylis JR, Ju HY, Thomson MH, Kazerooni A, Wang X, John AES, Lim EB, Chien D, Lee A, Zhang JQ (2015) Self-propelled particles that transport cargo through flowing blood and halt hemorrhage. Sci Adv 9:e1500379–e1500379

    Google Scholar 

  • Berce C, Muresan M-S, Soritau O, Petrushev B, Tefas L, Rigo I, Ungureanu G, Catoi C, Irimie A, Tomuleasa C (2018) Cutaneous wound healing using polymeric surgical dressings based on chitosan, sodium hyaluronate and resveratrol. A preclinical experimental study. Colloids Surf B Biointerfaces 163:155–166

    CAS  PubMed  Google Scholar 

  • Bonaventura G, Cognata V, Iemmolo R, Zimbone M, Contino A, Maccarrone G, Failla B, Barcellona ML, Francesca LC, D’Agata V (2018) Ag-NPs induce apoptosis, mitochondrial damages and MT3/OSGIN2 expression changes in an in vitro model of human dental-pulp-stem-cells-derived neurons. Neurotoxicology 67:84–93

    CAS  PubMed  Google Scholar 

  • Cai N, Li C, Han C, Luo X, Shen L, Xue Y, Yu F (2016) Tailoring mechanical and antibacterial properties of chitosan/gelatin nanofiber membranes with Fe3O4 nanoparticles for potential wound dressing application. Appl Surf Sci 369:492–500

    CAS  Google Scholar 

  • Cai N, Zeng H, Fu J, Chan V, Chen M, Li H, Yu F (2018) Synergistic effect of graphene oxide-silver nanofillers on engineering performances of polyelectrolyte complex nanofiber membranes. J Appl Polym Sci 19:46238

    Google Scholar 

  • Chang YC, Lee CJ, Wang LW, Wang YH (2018) Highly uniform resistive switching properties of solution-processed silver-embedded gelatin thin film. Small 14(13):1703888

    Google Scholar 

  • Feng C, Li J, Wu GS, Mu YZ, Kong M, Jiang CQ, Cheng XJ, Liu Y, Chen XG (2016) Chitosan-coated diatom silica as hemostatic agent for hemorrhage control. ACS Appl Mater Interfaces 50:34234

    Google Scholar 

  • Gowda S, Weinstein DA, Blalock TD, Gandhi K, Mast BA, Chin G, Schultz GS (2015) Topical application of recombinant platelet-derived growth factor increases the rate of healing and the level of proteins that regulate this response. Int Wound J 5:564–571

    Google Scholar 

  • Hattori H, Ishihara M (2017) Feasibility of improving platelet-rich plasma therapy by using chitosan with high platelet activation ability. Exp Ther Med 3:1176

    Google Scholar 

  • Hu S, Bi S, Yan D, Zhou Z, Sun G, Cheng X, Chen X (2018) Preparation of composite hydroxybutyl chitosan sponge and its role in promoting wound healing. Carbohyd Polym 184:154–163

    CAS  Google Scholar 

  • Jin J, Ji Z, Xu M, Liu C, Ye X, Zhang W, Li S, Wang D, Zhang W, Chen J (2018) Microspheres of carboxymethyl chitosan, sodium alginate, and collagen as a hemostatic agent in vivo. ACS Biomater Sci Eng 4(7):2541–2551

    CAS  Google Scholar 

  • Jun EA, Lim KM, Kim K, Bae ON, Noh JY, Chung KH, Chung JH (2011) Silver nanoparticles enhance thrombus formation through increased platelet aggregation and procoagulant activity. Nanotoxicology 2:157–167

    Google Scholar 

  • Kovach I, Rumschöttel J, Friberg SE, Koetz J (2016) Janus emulsion mediated porous scaffold bio-fabrication. Colloids Surf, B 145:347–352

    CAS  Google Scholar 

  • Li JL, Zarbock A, Hidalgo A (2017a) Platelets as autonomous drones for hemostatic and immune surveillance. J Exp Med 214(8):2193–2204

    CAS  PubMed Central  Google Scholar 

  • Li Q, Niu Y, Diao H, Wang L, Chen X, Wang Y, Dong L, Wang C (2017b) In situ sequestration of endogenous PDGF-BB with an ECM-mimetic sponge for accelerated wound healing. Biomaterials 148:54–68

    CAS  PubMed  Google Scholar 

  • Li Z, Zhou F, Li Z, Lin S, Chen L, Liu L, Chen Y (2018) Hydrogel cross-linked with dynamic covalent bonding and micellization for promoting burn wound healing. ACS Appl Mater Interfaces 30:25194–25202

    Google Scholar 

  • Liang D, Lu Z, Yang H, Gao J, Chen R (2016) A novel asymmetric wettable AgNPs/chitosan wound dressing: in vitro and in vivo evaluation. ACS Appl Mater Interfaces 6:3958

    Google Scholar 

  • Long M, Zhang Y, Huang P, Chang S, Hu Y, Yang Q, Mao L, Yang H (2017) Emerging nanoclay composite for effective hemostasis. Adv Func Mater 10:1704452

    Google Scholar 

  • Lu Z, Gao J, He Q, Wu J, Liang D, Yang H, Chen R (2017) Enhanced antibacterial and wound healing activities of microporous chitosan-Ag/ZnO composite dressing. Carbohyd Polym 156:460–469

    CAS  Google Scholar 

  • Ma Y, Xin L, Tan H, Fan M, Li J, Jia Y, Ling Z, Chen Y, Hu X (2017) Chitosan membrane dressings toughened by glycerol to load antibacterial drugs for wound healing. Mater Sci Eng, C 81:522–531

    CAS  Google Scholar 

  • Man WU (2018) Clinical efficacy of autologous platelet-rich gelatin in the treatment of patients with refractory diabetic skin ulcer and its effect on serum inflammatory factors. J Clin Med Pract 22:26–31

    Google Scholar 

  • Mishra SK, Raveendran S, Ferreira JM, Kannan S (2016) In situ impregnation of silver nanoclusters in microporous chitosan-PEG membranes as an antibacterial and drug delivery percutaneous device. Langmuir ACS J Surf Colloids 40:10305

    Google Scholar 

  • Mitra D, Li M, Wang R, Tang Z, Kang E-T, Neoh KG (2016) Scalable aqueous-based process for coating polymer and metal substrates with stable quaternized chitosan antibacterial coatings. Ind Eng Chem Res 36:9603–9613

    Google Scholar 

  • Nie W, Dai X, Li D, McCoul D, Gillispie GJ, Zhang Y, Yu B, He C (2018) One-pot synthesis of silver nanoparticle incorporated mesoporous silica granules for hemorrhage control and antibacterial treatment. ACS Biomater Sci Eng 10:3588–3599

    Google Scholar 

  • Pourjavadi A, Soleyman R (2011) Silver nanoparticles with gelatin nanoshells: photochemical facile green synthesis and their antimicrobial activity. J Nanopart Res 10:4647–4658

    Google Scholar 

  • Rodriguez-Menocal L, Shareef S, Salgado M, Shabbir A, Badiavas EV (2015) Role of whole bone marrow, whole bone marrow cultured cells, and mesenchymal stem cells in chronic wound healing. Stem Cell Res Ther 1:24

    Google Scholar 

  • Sakoda M, Kaneko M, Ohta S, Qi P, Ichimura S, Yatomi Y, Ito T (2018) Injectable hemostat composed of a polyphosphate-conjugated hyaluronan hydrogel. Biomacromol 8:3280–3290

    Google Scholar 

  • Sarhan WA, Azzazy HM, El-Sherbiny IM (2016) Honey/chitosan nanofiber wound dressing enriched with allium sativum and cleome droserifolia: enhanced antimicrobial and wound healing activity. ACS Appl Mater Interfaces 10:6379–6390

    Google Scholar 

  • Seon GM, Hee LM, Kwon BJ, Sung KM, Koo MA, Kim D, Seomun Y, Kim JT, Park JC (2017) Functional improvement of hemostatic dressing by addition of recombinant batroxobin. Acta Biomater 48:175–185

    CAS  PubMed  Google Scholar 

  • Shi X, Fang Q, Ding M, Wu J, Ye F, Lv Z, Jin J (2016) Microspheres of carboxymethyl chitosan, sodium alginate and collagen for a novel hemostatic in vitro study. J Biomater Appl 7:1092–1102

    Google Scholar 

  • Shi GN, Zhang CN, Xu R, Niu JF, Song HJ, Zhang XY, Wang WW, Wang YM, Li C, Wei XQ (2017) Enhanced antitumor immunity by targeting dendritic cells with tumor cell lysate-loaded chitosan nanoparticles vaccine. Biomaterials 113:191–202

    CAS  PubMed  Google Scholar 

  • Shimojo AAM, Perez AGM, Galdames SEM, Brissac ICS, Santana MHA (2016) Stabilization of porous chitosan improves the performance of its association with platelet-rich plasma as a composite scaffold. Mater Sci Eng, C 60:538–546

    CAS  Google Scholar 

  • Smock KJ, Schmidt RL, Hadlock G, Stoddard G, Grainger DW, Munger MA (2014) Assessment of orally dosed commercial silver nanoparticles on human ex vivo platelet aggregation. Nanotoxicology 3:328–333

    Google Scholar 

  • Sun X, Tang Z, Pan M, Wang Z, Yang H, Liu H (2017) Chitosan/kaolin composite porous microspheres with high hemostatic efficacy. Carbohydr Polym 177:135–143

    CAS  PubMed  Google Scholar 

  • Wang X, Yu T, Chen G, Zou J, Li J, Yan J (2017) Preparation and characterization of a chitosan/gelatin/extracellular matrix scaffold and its application in tissue engineering. Tissue Eng C Methods 3:169–179

    Google Scholar 

  • Wang X, Guan J, Zhuang X, Li Z, Huang S, Yang J, Liu C, Li F, Tian F, Wu J, Shu Z (2018) Exploration of blood coagulation of N-Alkyl chitosan nanofiber membrane in vitro. Biomacromol 3:731–739

    Google Scholar 

  • Xia L, Xu M, Cheng G, Yang L, Guo Y, Li D, Fang D, Zhang Q, Liu H (2018) Facile construction of Ag nanoparticles encapsulated into carbon nanotubes with robust antibacterial activity. Carbon 130:775–781

    CAS  Google Scholar 

  • Ye D, Zhong Z, Xu H, Chang C, Yang Z, Wang Y, Ye Q, Zhang L (2016) Construction of cellulose/nanosilver sponge materials and their antibacterial activities for infected wounds healing. Cellulose 1:1–15

    Google Scholar 

  • Yoo J, Birke A, Kim J, Jang Y, Song SY, Ryu S, Kim BS, Kim BG, Barz M, Char K (2018) Cooperative catechol-functionalized polypept(o)ide brushes and Ag nanoparticles for combination of protein resistance and antimicrobial activity on metal oxide surfaces. Biomacromol 5:1602

    Google Scholar 

  • Younes I, Sellimi S, Rinaudo M, Jellouli K, Nasri M (2014) Influence of acetylation degree and molecular weight of homogeneous chitosans on antibacterial and antifungal activities. Int J Food Microbiol 185:57–63

    CAS  PubMed  Google Scholar 

  • Zeng X, Mccarthy DT, Deletic A, Zhang X (2015) Silver/reduced graphene oxide hydrogel as novel bactericidal filter for point-of-use water disinfection. Adv Func Mater 27:4344–4351

    Google Scholar 

  • Zhang L, Ma Y, Pan X, Chen S, Zhuang H, Wang S (2018) A composite hydrogel of chitosan/heparin/poly (γ-glutamic acid) loaded with superoxide dismutase for wound healing. Carbohydr Polym 180:168–174

    CAS  PubMed  Google Scholar 

  • Zhang K, Bai X, Yuan Z, Cao X, Jiao X, Li Y, Qin Y, Wen Y, Zhang X (2019) Layered nanofiber sponge with an improved capacity for promoting blood coagulation and wound healing. Biomaterials 204:70–79

    CAS  PubMed  Google Scholar 

  • Zhao X, Lang Q, Yildirimer L, Lin ZY, Cui W, Annabi N, Ng KW, Dokmeci MR, Ghaemmaghami AM, Khademhosseini A (2016) Photocrosslinkable gelatin hydrogel for epidermal tissue engineering. Adv Healthc Mater 1:108–118

    Google Scholar 

  • Zhao X, Wu H, Guo B, Dong R, Qiu Y, Ma PX (2017) Antibacterial anti-oxidant electroactive injectable hydrogel as self-healing wound dressing with hemostasis and adhesiveness for cutaneous wound healing. Biomaterials 122:34–47

    CAS  PubMed  Google Scholar 

  • Zhao X, Guo B, Wu H, Liang Y, Ma PX (2018) Injectable antibacterial conductive nanocomposite cryogels with rapid shape recovery for noncompressible hemorrhage and wound healing. Nat Commun 9(1):2784

    PubMed  PubMed Central  Google Scholar 

  • Zhu J, Li F, Wang X, Yu J, Wu D (2018) Hyaluronic acid and polyethylene glycol hybrid hydrogel encapsulating nanogel with hemostasis and sustainable antibacterial property for wound healing. ACS Appl Mater Interfaces 16:13304–13316

    Google Scholar 

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 51703185) and the Key Research and Development Program (Social Development) of Zhenjiang City (No. SH2018001).

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  1. Guangqian Lan and Qing Li have equally contributed to this work.

Authors and Affiliations

  1. State Key Laboratory of Silkworm Genome Biology, College of Textile and Garments, Southwest University, Chongqing, 400715, China

    Guangqian Lan, Qing Li, Fei Lu, Kun Yu, Bitao Lu & Fangyin Dai

  2. Chongqing Engineering Research Center of Biomaterial Fiber and Modern Textile, Chongqing, 400715, China

    Guangqian Lan, Fei Lu & Fangyin Dai

  3. The Ninth People’s Hospital of Chongqing, Chongqing, 400715, China

    Rong Bao

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  1. Guangqian Lan
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Correspondence to Guangqian Lan.

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Lan, G., Li, Q., Lu, F. et al. Improvement of platelet aggregation and rapid induction of hemostasis in chitosan dressing using silver nanoparticles. Cellulose 27, 385–400 (2020). https://doi.org/10.1007/s10570-019-02795-1

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