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Halobellus ruber sp. nov., a deep red-pigmented extremely halophilic archaeon isolated from a Korean solar saltern

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Abstract

A novel halophilic archaeon, strain MBLA0160T, was isolated from a solar saltern in Sorae, Republic of Korea. The cells are deep-red pigmented, Gram-negative, rod shaped, motile, and lysed in distilled water. The strain MBLA0160T grew at 25–45 °C (optimum 37 °C), in 15–30% (w/v) NaCl (optimum 20%) and 0.1–1.0 M MgCl2 (optimum 0.3–0.5 M) at pH 5.0–9.0 (optimum 7.0). Phylogenetic analysis based on the 16S rRNA sequence showed that this strain was related to two species within the genus Halobellus (Hbs.), with 98.4% and 95.8% similarity to Hbs. salinus CSW2.24.4 T and Hbs. clavatus TNN18T, respectively. The major polar lipids of the strain MBLA160T were phosphatidylglycerol, phosphatidylglycerol sulfate, and phosphatidylglycerol phosphate methyl ester. The genome size, G + C content, and N50 value of MBLA0160T were 3.49 Mb, 66.5 mol%, and 620,127 bp, respectively. According to predicted functional proteins of strain MBLA0160T, the highest category was amino acid transport and metabolism. Genome rapid annotation showed that amino acid and derivatives was the most subsystem feature counts. Pan-genomic analysis showed that strain MBLA0160T had 97 annotated unique KEGG, which were mainly included metabolism and environmental information processing. Ortholog average nucleotide identities (OrthoANI) and in silico DNA-DNA hybridization (isDDH) values between the strain MBLA0160T and other strains of the genus Halobellus were under 84,4% and 28.1%, respectively. The genome of strain MBLA0160T also contain the biosynthetic gene cluster for C50 carotenoid as secondary metabolite. Based on the phylogenetic, phenotypic, chemotaxonomic properties, and comparative genomic analyses, strain MBLA0160T is considered to represent a novel species of the genus Halobellus, for which the name Halobellus ruber sp. nov. is proposed. The type strain is MBLA0160T (= KCTC 4291 T = JCM 34172 T).

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Funding

This work was supported by an Incheon National University Research Grant in 2018.

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  1. ChiYoung Hwang and Eui-Sang Cho contributed equally to this work and should be regarded as co-first authors.

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  1. Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University, Incheon, 22012, Republic of Korea

    Chi Young Hwang, Eui-Sang Cho, Deok Jun Yoon & Myung-Ji Seo

  2. Division of Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea

    Myung-Ji Seo

  3. Institute for New Drug Development, Incheon National University, Incheon, 22012, Republic of Korea

    Myung-Ji Seo

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C Y H and E-S C performed all the experiments and drafted the manuscript. D J Y contributed to isolate the strains and analyze the data. M-J S designed all the experiments and supervised the manuscript.

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Correspondence to Myung-Ji Seo.

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Hwang, C.Y., Cho, ES., Yoon, D.J. et al. Halobellus ruber sp. nov., a deep red-pigmented extremely halophilic archaeon isolated from a Korean solar saltern. Antonie van Leeuwenhoek 114, 997–1011 (2021). https://doi.org/10.1007/s10482-021-01571-1

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