Abstract
Somatolactin (SL) is a pituitary hormone belonging to the growth hormone–prolactin family and is produced in the intermediate lobe of teleosts. The SL gene was isolated from a sea bream genomic library and found to be composed of 5 exons distributed within a 9-kb length of DNA. Sequence analysis of the proximal promoter region showed the presence of a classical TATA box located 59 bp upstream from the initial start ATG codon, 5 consensus sequences corresponding to the Pit-1 binding element, and a putative CREB site. In CHO cells cotransfected with the DNA from 2 plasmids, one encoding sea bream Pit-1 under Rous sarcoma virus long terminal repeat regulation and one encoding the SL promoter driving the expression of luciferase, Pit-1 was found to enhance the expression of luciferase. Only one Pit-1 binding site was necessary for enhancement. Analysis by immunoblots of in vitro culture of pituitaries of Sparus aurata showed that several agents, including estradiol, verapamil, and phorbol myristate acetate, had different inhibitory effects on SL and growth hormone released to the culture medium.
Similar content being viewed by others
References
L.B. Agellon S.L. Davies T.T. Chen D.A. Powers (1988) ArticleTitleStructure of a fish (rainbow trout) growth hormone gene and its evolutionary implications Proc Natl Acad Sci U S A 239 1400–1405
Y. Amemiya Y. Sogabe M. Nozaki A. Takahashi H. Kawauchi (1999) ArticleTitleSomatolactin in the white sturgeon and African lungfish and its evolutionary significance Gen Comp Endocrinol 114 181–190 Occurrence Handle10.1006/gcen.1998.7250 Occurrence Handle1:CAS:528:DyaK1MXisVaisLw%3D Occurrence Handle10208767
A. Astola C. Pendón M. Ortiz M.M. Valdivia (1996) ArticleTitleCloning and expression of somatolactin a pituitary hormone related to growth hormone and prolactin from gilthead seabream Sparus aurata Gen Comp Endocrinol 104 330–336 Occurrence Handle10.1006/gcen.1996.0178 Occurrence Handle1:CAS:528:DyaK28XntlejtLg%3D Occurrence Handle8954766
J.N. Ball T.F.C. Batten (1981) ArticleTitlePituitary and melanophore responses to background in Poecilla latiginna (Teleosttei): role of the pars intermedia PAS cells Gen Comp Endocrinol 44 233–248 Occurrence Handle1:CAS:528:DyaL3MXkslOgsLk%3D Occurrence Handle7250687
R.N. Day S. Koike M. Sakai M. Muramatsu RA. Maurer (1990) ArticleTitleBoth Pit-1 and the estrogen receptor are required for estrogen reponsiveness of the rat prolactin gene Mol Endocrinol 4 1964–1971 Occurrence Handle1:CAS:528:DyaK3MXkvVGlsLo%3D Occurrence Handle2082192
M.J. Ford (2000) ArticleTitleEffects of natural selection on patterns of DNA sequence variation at the transferrin, somatolactin and p53 genes within and among Chinook salmon (Oncorhynchus tshawytscha) populations Mol Ecol 9 843–855 Occurrence Handle10.1046/j.1365-294x.2000.00933.x Occurrence Handle1:CAS:528:DC%2BD3cXlslWqur0%3D Occurrence Handle10886648
H.A. Ingraham S.E. Flynn J.W. Voss V.R. Albert M.S. Kapiloff L. Wilson M.G. Rosenfeld (1990) ArticleTitleThe POU-specific domain of Pit-1 is essential for sequence-specific high affinity DNA binding and DNA-dependent Pit-1–Pit-1 interactions Cell 61 1021–1033 Occurrence Handle10.1016/0092-8674(90)90067-O Occurrence Handle1:CAS:528:DyaK3cXkslagtrg%3D Occurrence Handle2350782
F. Iraqi Z. Gong C.L. Hew (1993) ArticleTitleIsolation and characterization of somatolactin genes from two cold water marine teleost, lumpfish (Cyclopterus lumpus) and halibut (Hippoglossus hippoglossus) Mol Mar Biol Biotech 2 96–103
H. Kaji S. Tai Y. Okimura G. Iguchi Y. Takahashi H. Abe K. Chihara (1998) ArticleTitleCloning and characterization of the 5”-flanking region of the human growth hormone secretagogue receptor gene J Biol Chem 273 33885–33888 Occurrence Handle10.1074/jbc.273.51.33885 Occurrence Handle1:CAS:528:DyaK1MXnt1Gm Occurrence Handle9852035
T. Kaneko T. Hirano (1993) ArticleTitleRole of prolactin and somatolactin in calcium regulation in fish J Exp Biol 184 31–45 Occurrence Handle1:CAS:528:DyaK2cXisVSmsr0%3D
M. Karin J.L. Castrillo L.E. Theill (1990) ArticleTitleGrowth hormone regulation: a paradigm for cell-type-specific gene activation Trends Genet 6 92–96 Occurrence Handle10.1016/0168-9525(90)90100-K Occurrence Handle1:CAS:528:DyaK3cXhvFaks7s%3D Occurrence Handle2183418
M.K. Kim J.H. McClaskey D.L. Bodenner B.D. Weintraub (1993) ArticleTitleAn AP-1 like factor and the pituitary-specific factor Pit-1 are both necessary to mediate hormonal induction of human thyrotropin beta gene expression J Biol Chem 268 23366–23375 Occurrence Handle1:CAS:528:DyaK3sXlslCnsb4%3D Occurrence Handle8226861
L. Liang T. Major T. Bocan (2002) ArticleTitleCharacterization of the promoter of human extracellular matrix metalloproteinase inducer (EMMPRIN) Gene 282 75–86 Occurrence Handle10.1016/S0378-1119(01)00847-2 Occurrence Handle1:CAS:528:DC%2BD38XnvFSgsw%3D%3D Occurrence Handle11814679
J.P. Martínez-Barberá C. Pendón R.B. Rodríguez J. Pérez-Sánchez M.M. Valdivia (1994) ArticleTitleCloning expression and characterization of a recombinant gilthead seabream growth hormone Gen Comp Endocrinol 96 179–188 Occurrence Handle10.1006/gcen.1994.1172 Occurrence Handle7851717
J.P. Martínez-Barberá V. Vila M.M. Valdivia J.L. Castrillo (1997) ArticleTitleMolecular cloning of gilthead seabream (Sparus aurata) transcription factor GHF-1/Pit-1 Gene 185 87–93 Occurrence Handle10.1016/S0378-1119(96)00634-8 Occurrence Handle9034317
T. Ohkubo M. Araki M. Tanaka S. Sudo K. Nakashima (1996) ArticleTitleMolecular cloning and characterization of the yellowtail GH gene and its promoter: a consensus sequence for teleost and avian Pit-1/GHF-1 binding sites J Mol Endocrinol 16 63–72 Occurrence Handle1:CAS:528:DyaK28Xht1Shtrs%3D Occurrence Handle8672234
M. Olivereau C. Aiamr J.M. Olivereau (1980) ArticleTitleResponse of the teleost pituitary (goldfish, eel) to deionized water Cell Tissue Res 208 389–404 Occurrence Handle10.1007/BF00233872 Occurrence Handle1:CAS:528:DyaL3cXkvFahsbY%3D Occurrence Handle7397761
M. Ono Y. Takayama (1992) ArticleTitleStructures of cDNAs encoding chum salmon pituitary-specific transcription factor Pit-l/GHF-1 Gene 226 275–279 Occurrence Handle10.1016/0378-1119(92)90525-T
M. Ono Y. Takayama M. Rand-Weaver S. Sakata T. Yasunaga T. Noso H. Kawauchi (1990) ArticleTitlecDNA cloning of somatolactin pituitary protein related to growth hormone and prolactin Proc Natl Acad Sci U S A 87 4330–4334
M. Ono T. Harigai T. Kaneko Y. Sato S. Ihara H. Kawauchi (1994) ArticleTitlePit-1/GH factor-1 involvement in the gene expression of somatolactin Mol Endocrinol 110 109–115 Occurrence Handle10.1210/me.8.1.109
C. Pendón J.P. Martínez-Barberá M.M. Valdivia (1994) ArticleTitleCloning of a somatolactin-encoding cDNA from sole (Solea senegalensis) Gene 147 227–230 Occurrence Handle10.1016/0378-1119(94)90071-X Occurrence Handle7926805
M. Rand-Weaver T. Noso K. Muramoto H. Kawauchi (1991) ArticleTitleIsolation and characterization of somatolactin a new protein related to growth hormone and prolactin from Atlantic cod (Gadus morhua) pituitary glands Biochemistry 30 1509–1515 Occurrence Handle1:CAS:528:DyaK3MXnslantQ%3D%3D Occurrence Handle1993170
M. Rand-Weaver P. Swanson H. Kawauchi W.W. Dickhoff (1992) ArticleTitleSomatolactin, a novel pituitary protein: purification and plasma levels during reproductive maturation of coho salmon J Endocrinol 133 393–403 Occurrence Handle1:CAS:528:DyaK38XktVCls70%3D Occurrence Handle1613440
M. Rand-Weaver T.C. Pottinger J.P. Sumpter (1993) ArticleTitlePlasma somatolactin concentrations in salmonid fish are elevated by stress J Endocrinol 138 509–515 Occurrence Handle1:CAS:528:DyaK2cXlt1ym Occurrence Handle8277224 Occurrence Handle10.1677/joe.0.1380509
J.L. Specker D.S. King R.S. Nishioka K. Shirahata K. Yamaguchi H.A. Bern (1985) ArticleTitleIsolation and partial characterization of a pair of prolactins released in vitro by the pituitary of a cichlid fish Orchocronus mossambicus Proc Natl Acad Sci U S A 82 7490–7494
Y. Takayama M Rand-Weaver H. Kawauchi M. Ono (1991a) ArticleTitleGene structure of chum salmon somatolactin, a presumed pituitary hormone of the growth hormone/prolactin family Mol Endocrinol 5 778–786 Occurrence Handle1:CAS:528:DyaK38XhtVSgur4%3D
Y. Takayama M. Ono M. Rand-Weaver H. Kawauchi (1991b) ArticleTitleGreater conservation of somatolactin, a presumed pituitary hormone of the growth hormone/prolactin family, than growth hormone in teleost fish Gen Comp Endocrinol 83 366–374 Occurrence Handle1:CAS:528:DyaK38XhsVymsL4%3D
H. Towbin T. Staehelin J. Gordon (1979) ArticleTitleElectrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications Proc Natl Acad Sci U S A 76 4350–4354 Occurrence Handle1:CAS:528:DyaE1MXmtVKltLw%3D Occurrence Handle388439
M.M. Valdivia C. Pendón A. Astola J Pérez-Sánchez (1998) ArticleTitleMolecular and physiological studies of the teleost somatolactin: a review Trends Comp Biochem Physiol 5 171–177 Occurrence Handle1:CAS:528:DC%2BD3cXls12ntw%3D%3D
S. Yamada J. Hata S. Yamashita (1993) ArticleTitleMolecular cloning of fish Pit-1 cDNA and its functional binding to promoter of gene expressed in the pituitary J Biol Chem 268 24361–24366 Occurrence Handle1:CAS:528:DyaK3sXlvFOjsLk%3D Occurrence Handle8226986
Acknowledgments
This work was supported by the Ministerio de Ciencia y Tecnología (PGC 98-0672) and partially by the Plan Andaluz de Investigación of the Junta de Andalucía (group CVI 167). We are indebted to CASEM (University of Cádiz) for supplying us with specimens of Sparus aurata.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Astola, A., Calduch-Giner, J.A., Ortiz, M. et al. Genomic Structure and Functional Analysis of Promoter Region of Somatolactin Gene of Sea Bream (Sparus aurata). Mar Biotechnol 6, 411–418 (2004). https://doi.org/10.1007/s10126-004-3210-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10126-004-3210-2