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Production of protein hydrolysate from Protaetia brevitarsis seulensis (Kolbe) larvae by enzyme treatment under high pressure

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Abstract

To improve the industrial use of health-functional materials based on edible insects, the objective of this study was to establish optimal conditions for improving the quality of Protaetia brevitarsis seulensis larval (PBSL) hydrolysates. PBSL was extracted using four methodologies: atmospheric pressure 50 °C-water extraction, atmospheric pressure 95 °C-water extraction, atmospheric pressure 50 °C-water enzymatic hydrolysis, and enzyme treatment under high pressure (HPE). The quality characteristics of soluble solid content, extraction yield, total protein content, protein yield, protein content with low molecular weight (LMW) (< 1kD), and the amino acid composition of hydrolysates were compared based on the different methods. All of the quality characteristics were found to be higher for HPE extracts than for the other extracts. Under optimized HPE conditions, extraction yield, protein yield, protein content with LMW, amino acid content and the content of essential amino acids increased by 3.4, 4.4 1.4 1.5, and 1.3 times respectively, compared to the other methods.

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Acknowledgements

This work was supported by the Technology Development Program (P0002762) funded by the Ministry of SMEs andStartups (MSS, Korea) and the Main Research Program (Grant number: E0187000-03) of the Korea Food ResearchInstitute (KFRI) funded by the Ministry of Science and ITC, Republic of Korea.

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Authors and Affiliations

  1. Korea Food Research Institute, Wanju-Gun, Jeollabuk-do, 55365, Republic of Korea

    Tae-Eun Kim & Yong-Jin Cho

  2. R&D Center, EastHill Corporation, Suwon, Gyeonggi-do, 16642, Republic of Korea

    Chong-Tai Kim

  3. Research Institute Wonkwang Herb Co., Ltd, Jinan-Gun, Jeollabuk-do, Republic of Korea

    Hyeon-Jae Sim, Hoon-Yeon Lee, Seong-Oh Kim, Dong-Keun Kim, Mi-Na Jo & So-Jin Lee

  4. Department of Oriental Medicine Resource, Chonbuk National University, 79 Gobongro, Iksan, Jeollabuk-do, 54596, Republic of Korea

    Yong-Deok Jeon, Young-Jae Song & Jong-Sik Jin

  5. Korea Zoonosis Research Institute, Chonbuk National University, Iksan, Jeollabuk-do, 54531, Republic of Korea

    Yong-Deok Jeon

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  1. Tae-Eun Kim
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  2. Chong-Tai Kim
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Correspondence to Yong-Jin Cho.

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Kim, TE., Kim, CT., Sim, HJ. et al. Production of protein hydrolysate from Protaetia brevitarsis seulensis (Kolbe) larvae by enzyme treatment under high pressure. Food Sci Biotechnol 29, 1187–1194 (2020). https://doi.org/10.1007/s10068-020-00766-y

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  • DOI: https://doi.org/10.1007/s10068-020-00766-y

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