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Response to adverse conditions in two strains of the extremely halophilic species Salinibacter ruber

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Abstract

We have studied the response of the two closest relative strains M8 and M31 of Salinibacter ruber to environmental changes as the transition from exponential to stationary phase in a batch growth, and the submission to two different environmental stresses (dilution of the culture medium and temperature decrease). We monitored the changes in cultivability, ribosomal content by fluorescence in situ hybridization (FISH), and metabolic changes with high-field ion cyclotron Fourier transform mass spectrometry. In all cases, we could observe an important decrease in cultivability that was not accompanied by a decrease in FISH counts, pointing to a transition to viable but non-cultivable state rather than cell death. Furthermore, the metabolomic analyses indicated a common response of both strains to the different conditions assayed. Only a small portion of the detected masses could be annotated due to database constraints. Among them, the most remarkable changes could be attributed to modifications in the composition of the cell envelope, and especially in the cell membrane. We could track changes in the length or saturation of the fatty acids and in the composition of phospholipids involved in aminosugar, glycerolipid, and glycerophospholipid metabolic pathways.

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Acknowledgments

This work was supported by the projects CLG2009_12651-C02-01 and 02; and CE-CSD2007-0005 of the Spanish Ministry of Science and Innovation, and all three projects were also co-financed with FEDER support from the European Union. J. Brito-Echeverría was financed by the Government of the Balearic Islands, Ministry of Economy and Finance.

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Authors and Affiliations

  1. Marine Microbiology Group, Institut Mediterrani d’ Estudis Avançats (CSIC-UIB), 07190, Esporles, Spain

    Jocelyn Brito-Echeverría, Arantxa López-López & Ramón Rosselló-Móra

  2. Helmholtz Zentrum Muenchen, German Research Center for Environmental Health, Institute of Ecological Chemistry, Neuherberg, Germany

    Marianna Lucio & Philippe Schmitt-Kopplin

  3. Departamento de Fisiología, Genética y Microbiología and Instituto Multidisciplinar de Estudios del Medio, Universidad de Alicante, Alicante, Spain

    Josefa Antón

Authors
  1. Jocelyn Brito-Echeverría
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  2. Marianna Lucio
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  3. Arantxa López-López
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  4. Josefa Antón
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  5. Philippe Schmitt-Kopplin
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  6. Ramón Rosselló-Móra
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Correspondence to Jocelyn Brito-Echeverría.

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Communicated by A. Oren.

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Brito-Echeverría, J., Lucio, M., López-López, A. et al. Response to adverse conditions in two strains of the extremely halophilic species Salinibacter ruber . Extremophiles 15, 379–389 (2011). https://doi.org/10.1007/s00792-011-0366-3

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  • DOI: https://doi.org/10.1007/s00792-011-0366-3

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