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Bioactive glass-ceramic bone repair associated or not with autogenous bone: a study of organic bone matrix organization in a rabbit critical-sized calvarial model

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Abstract

Objective

The aim of the study was to analyze bone matrix (BMX) organization after bone grafting and repair using a new bioactive glass-ceramic (Biosilicate®) associated or not with particulate autogenous bone graft.

Material and methods

Thirty rabbits underwent surgical bilateral parietal defects and divided into groups according to the materials used: (C) control—blood clot, (BG) particulate autogenous bone, (BS) bioactive glass-ceramic, and BG + BS. After 7, 14, and 30 days post-surgery, a fragment of each specimen was fixed in − 80 °C liquid nitrogen for zymographic evaluation, while the remaining was fixed in 10% formalin for histological birefringence analysis.

Results

The results of this study demonstrated that matrix organization in experimental groups was significantly improved compared to C considering collagenous organization. Zymographic analysis revealed pro-MMP-2, pro-MMP-9, and active (a)-MMP-2 in all groups, showing gradual decrease of total gelatinolytic activity during the periods. At day 7, BG presented more prominent gelatinolytic activity for pro-MMP-2 and 9 and a-MMP-2, when compared to the other groups. In addition, at day 7, a 53% activation ratio (active form/[active form + latent form]) was evident in C group, 33% in BS group, and 31% in BG group.

Conclusion

In general, BS allowed the production of a BMX similar to BG, with organized collagen deposition and MMP-2 and MMP-9 disponibility, permitting satisfactory bone remodeling at the late period.

Clinical relevance

The evaluation of new bone substitute, with favorable biological properties, opens the possibility for its use as a viable and efficient alternative to autologous bone graft.

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Acknowledgements

The authors are grateful to Maira Cristina Rondina Couto for histology assistance.

Funding

This work was supported by São Paulo Research Foundation (FAPESP/SP), grant numbers 2008/11485-8; 2009/17294-1.

Author information

Authors and Affiliations

  1. Research and Postgraduate Pro-Rectory, Universidade do Sagrado Coração – USC, Rua Irmã Arminda 10-50, Jardim Brasil Bauru, SP, 17011-160, Brazil

    Claudia Cristina Biguetti & Patrícia Pinto Saraiva

  2. Oral Biology Doctoral’s Program, Bauru School of Dentistry, São Paulo University – FOB/USP, Alameda Octávio Pinheiro Brizola 9-75, Vila Universitária, Bauru, SP, 17012-901, Brazil

    Claudia Cristina Biguetti

  3. Department of Conservative Dentistry, School of Dentistry, Universidad de Chile, Sergio Livingstone 943, Recoleta, Santiago, RM, Chile

    Franco Cavalla

  4. Biotechnology Doctoral’s Program, São Carlos Federal University – UFSCAR, Rodovia Washington Luís, km 235, SP-310, São Carlos, SP, 13565-905, Brazil

    Carla Roberta Tim

  5. Molecular Biology Laboratory, Research and Postgraduate Pro-Rectory, Universidade do Sagrado Coração – USC, Rua Irmã Arminda 10-50, Jardim, 17011-160, Brazil

    Wilson Orcini

  6. Discipline of Oral and Maxillofacial Surgery, Department of Health Sciences, Universidade Sagrado Coração – USC, Rua Irmã Arminda 10-50, Jardim Brasil Bauru, SP, 17011-160, Brazil

    Leandro De Andrade Holgado

  7. Department of Bioscience, Federal University of São Paulo, Campus Baixada Santista - UNIFESP, Avenida Ana Costa, 95 – Vila Matias, Santos, SP, 11060-001, Brazil

    Ana Claudia Muniz Rennó

  8. Discipline of Histology and Embriology, Department of Basic Sciences, São Paulo State University – FOA/UNESP, Rua Paul Harris 1100, Casa 03, Jardim Nova Iorque Araçatuba, SP, 18016-110, Brazil

    Mariza Akemi Matsumoto

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  1. Claudia Cristina Biguetti
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Correspondence to Claudia Cristina Biguetti.

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All procedures performed in studies involving animals were in accordance with the ethical standards of the Ethical Committee for Animal Research of Sagrado Coração University (protocol # 110/09), and the Brazilian College of Animal Experimentation (COBEA) guidelines for the care and use of laboratory animals.

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Biguetti, C.C., Cavalla, F., Tim, C.R. et al. Bioactive glass-ceramic bone repair associated or not with autogenous bone: a study of organic bone matrix organization in a rabbit critical-sized calvarial model. Clin Oral Invest 23, 413–421 (2019). https://doi.org/10.1007/s00784-018-2450-x

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