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Carious deciduous teeth are a potential source for dental pulp stem cells

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Abstract

Objective

The objectives of this study are to isolate, cultivate, and characterize stem cells from the pulp of carious deciduous teeth (SCCD) and compare them to those retrieved from sound deciduous teeth (SHED—stem cells from human exfoliated deciduous teeth).

Material and methods

Cells were obtained of dental pulp collected from sound (n = 10) and carious (n = 10) deciduous human teeth. Rate of isolation, proliferation assay (0, 1, 3, 5, and 7 days), STRO-1, mesenchymal (CD29, CD73, and CD90) and hematopoietic surface marker expression (CD14, CD34, CD45, HLA-DR), and differentiation capacity were evaluated.

Results

Isolation success rates were 70 and 80 % from the carious and sound groups, respectively. SCCD and SHED presented similar proliferation rate. There were no statistical differences between the groups for the tested surface markers. The cells from sound and carious deciduous teeth were positive for CD29, CD73, and CD90 and negative for CD14, CD34, CD45, and HLA-DR and were capable of differentiating into osteogenic, chondrogenic, and adipogenic lineages.

Conclusion

SCCD demonstrated a similar pattern of proliferation, immunophenotypical characteristics, and differentiation ability as those obtained from sound deciduous teeth. These SCCD represent a feasible source of stem cells.

Clinical relevance

Decayed deciduous teeth have been usually discarded once the pulp tissue could be damaged and the activity of stem cells compromised. These findings show that stem cells from carious deciduous teeth can be applicable source for cell-based therapies in tissue regeneration.

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Acknowledgments

The authors wish to thank Andrea Galuppo and Pedro Chagastelles for their assistance in the flow cytometry procedures.

Compliance with ethical standards

ᅟ

Funding

This study was funded by the National Council for Scientific and Technological Development—CNPq (process no. 478778/2011-2).

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in the present research were in accordance with the ethical standards of the Resolution of the National Council on Ethics in Research (no. 466, /2012) and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study. The protocol of this research was submitted and approved by the Research Committee (no. 22972) and the Ethics Committee (no. 04317812.8.0000.5347) of Federal University of Rio Grande do Sul, Porto Alegre, RS—Brazil.

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Authors and Affiliations

  1. Department of Pediatric Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil

    Stefanie Bressan Werle, Daniele Lindemann, Fernando Borba de Araujo & Luciano Casagrande

  2. Stem Cell Laboratory and Stem Cell Research Institute, School of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, Brazil

    Daniela Steffens & Patrícia Pranke

  3. Department of Operatory Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil

    Flávio Fernando Demarco

  4. Head of Hematology and Stem Cell Laboratory and Stem Cell Research Institute, School of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, Brazil

    Patrícia Pranke

Authors
  1. Stefanie Bressan Werle
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  2. Daniele Lindemann
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  3. Daniela Steffens
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  4. Flávio Fernando Demarco
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  5. Fernando Borba de Araujo
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  7. Luciano Casagrande
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Corresponding author

Correspondence to Luciano Casagrande.

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Werle, S.B., Lindemann, D., Steffens, D. et al. Carious deciduous teeth are a potential source for dental pulp stem cells. Clin Oral Invest 20, 75–81 (2016). https://doi.org/10.1007/s00784-015-1477-5

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  • DOI: https://doi.org/10.1007/s00784-015-1477-5

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