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A pilot study on an attenuated Chinese EIAV vaccine inducing broadly neutralizing antibodies

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Abstract

The attenuated Chinese equine infectious anemia virus (EIAV) vaccine has successfully protected millions of equine animals from EIA disease in China. In this pilot study, to determine whether this attenuated vaccine can induce broadly neutralizing antibodies, we immunized four horses with the attenuated Chinese vaccine strain EIAVFDDV and then observed the evolution of neutralizing antibodies against different EIAV strains. During the vaccination phase, all vaccinees rapidly developed high levels of neutralizing antibodies against the homologous vaccine strain (pLGFD3V), and 3 out of 4 horses showed a gradual increase in serum neutralizing activity against two relatively heterologous virulent variants of the challenge strain (pLGFD3Mu12V and DLV34). After challenge, the three horses that had developed high levels of neutralizing antibodies against pLGFD3Mu12V and DLV34 did not show signs of infection, which was demonstrated by immune suppression, while the one horse producing serum that could only neutralize pLGFD3V developed a febrile episode during the 8-month observation period. To assess whether the broadly neutralizing activity is associated with immune protection, sera drawn on the day of challenge from these four vaccinees and an additional four EIAVFDDV-vaccinated horses were analyzed for neutralizing antibodies against pLGFD3V, pLGFD3Mu12V and DLV34. Although there was no significant correlation between protection from infection and serum neutralizing activity against any of these three viral strains, protection from infection was observed to correlate better with serum neutralizing activity against the two heterologous virulent strains than against the homologous vaccine strain. These data indicate that EIAVFDDV induced broadly neutralizing antibodies, which might confer enhanced protection of vaccinees from infection by the challenge virus.

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Acknowledgments

This work was supported by grants from the Ministry of Science and Technology of China (Grant No. 2007DFC30230, 2008ZX10001-010) to Dr. Yiming Shao and National Science Foundation of China (Grant No. 30371319 and 30671941) to Dr. Xiaoyan Zhang. We sincerely appreciate the critical comments on the paper provided by Dr. Douglas B. Lowrie, Dr. Jianqing Xu, Dr. Yong Gao and Dr. Pingfu Fu and the editorial assistance provided by Dr. Donald D. Anthony.

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Authors and Affiliations

  1. State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China

    Qinglai Meng, Yan Ma, Shenwei Li, Hua Liang, Xiaoyan Zhang & Yiming Shao

  2. National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 150001, Harbin, China

    Yuezhi Lin, Jian Ma, Liping Zhao, Jianhua Zhou & Rongxian Shen

  3. Shanghai Public Health Clinical Center, Institutes of Biomedical Sciences, Fudan University, 201508, Shanghai, China

    Xiaoyan Zhang

Authors
  1. Qinglai Meng
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  2. Yuezhi Lin
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  3. Jian Ma
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  4. Yan Ma
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  5. Liping Zhao
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  6. Shenwei Li
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  7. Hua Liang
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  8. Jianhua Zhou
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  9. Rongxian Shen
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  10. Xiaoyan Zhang
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  11. Yiming Shao
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Corresponding authors

Correspondence to Xiaoyan Zhang or Yiming Shao.

Additional information

Qinglai Meng and Yuezhi Lin contributed equally to this paper.

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Meng, Q., Lin, Y., Ma, J. et al. A pilot study on an attenuated Chinese EIAV vaccine inducing broadly neutralizing antibodies. Arch Virol 156, 1455–1462 (2011). https://doi.org/10.1007/s00705-011-0992-1

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  • DOI: https://doi.org/10.1007/s00705-011-0992-1

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