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Protective effect of urapidil on testicular torsion–detorsion injury in rats

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Abstract

Purpose

The aim of this study was to investigate the effect of urapidil and low-molecular weight heparin (LMWH) on testicular torsion–detorsion injury in rats.

Methods

Thirty-two male Sprague–Dawley rats were used. In the torsion–detorsion (T/D) group, the left testis was twisted at 720° for 3 h. After 3 h of reperfusion, the testis was removed. Urapidil or LMWH was administered intraperitoneally 30 min before detorsion in the treatment groups.

Results

Unilateral testicular torsion–detorsion caused significant increases in the malondialdehyde level and apoptosis and significant decreases in the superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities in ipsilateral testes (p < 0.001). The rats treated with urapidil had a significant decrease in the malondialdehyde level and apoptosis and significant increases in the SOD and GPx activities in ipsilateral testes compared to the T/D group (p < 0.001). Animals treated with LMWH showed non-significant reductions in malondialdehyde levels and apoptosis compared to the T/D group. In addition, no significant difference in the SOD activities (p = 0.52) between the groups was found. The increase in the GPx activities was significant in the LMWH group compared to the T/D group (p < 0.001).

Conclusion

The administration of urapidil before detorsion prevents ischemia/reperfusion cellular damage in testicular tissue. LMWH was not found to have a beneficial effect on testicular T/D injury in rats.

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Authors and Affiliations

  1. Department of Pediatric Surgery, Split University Hospital Centre and Split University School of Medicine, Spinčićeva 1, 21 000, Split, Croatia

    Jakov Meštrović, Zenon Pogorelić & Davor Todorić

  2. Department of Medical Chemistry and Biochemistry, University of Split School of Medicine, Spinčićeva 1, 21 000, Split, Croatia

    Irena Drmić-Hofman

  3. Department of Pathology, Forensic Medicine and Cytology, Split University Hospital Centre and Split University School of Medicine, Spinčićeva 1, 21 000, Split, Croatia

    Irena Drmić-Hofman, Katarina Vilović & Marijana Popović

Authors
  1. Jakov Meštrović
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  2. Zenon Pogorelić
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  3. Irena Drmić-Hofman
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  4. Katarina Vilović
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  5. Davor Todorić
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  6. Marijana Popović
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Correspondence to Zenon Pogorelić.

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The authors declared no potential conflicts of interest with respect to the research, authorship, or publication of this article.

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Meštrović, J., Pogorelić, Z., Drmić-Hofman, I. et al. Protective effect of urapidil on testicular torsion–detorsion injury in rats. Surg Today 47, 393–398 (2017). https://doi.org/10.1007/s00595-016-1388-3

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  • DOI: https://doi.org/10.1007/s00595-016-1388-3

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