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Titanium and tantalum as mesenchymal stem cell scaffolds for spinal fusion: an in vitro comparative study

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Abstract

Introduction

In the last few years, great interest has been focused on tissue engineering as a potential therapeutic approach for musculoskeletal diseases. The role of metallic implants for spinal fusion has been tested in preclinical and clinical settings. Titanium and tantalum have excellent biocompatibility and mechanical properties and are being used in this situation. On the other hand, the therapeutic role of mesenchymal stem cells (MSC) is extensively explored for their multilineage differentiation into osteoblasts.

Objetives

In vitro comparision of titanium and tantalum as MSCs scaffolds.

Material and methods

In the present study, we have compared the in vitro expansion capacity, viability, immunophenotype (both explored by flow cytometry) and multi-differentiation ability of MSC cultured in the presence of either titanium or tantalum fragments. The adherence of MSC to either metal was demonstrated by electron microscopy.

Results

Both metals were able to carry MSC when transferred to new culture flasks. In addition, our study shows that culture of MSC with titanium or tantalum improves cell viability and maintains all their biological properties, with no significant differences regarding the metal employed.

Conclusion

This would support the use of these combinations for clinical purposes, especially in the spinal fusion and reconstruction setting.

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Authors and Affiliations

  1. Department of Orthopedics, Servicio de Traumatología y CO, Hospital Universitario de Salamanca, Paseo de San Vicente 58, 37007, Salamanca, Spain

    Juan F. Blanco

  2. Department of Hematology, Hospital Universitario de Salamanca, Paseo de San Vicente 58, 37007, Salamanca, Spain

    Fermin M. Sánchez-Guijo & Maria-Consuelo del Cañizo

  3. Cell Therapy Lab, Hospital Universitario de Salamanca, Paseo de San Vicente 58, 37007, Salamanca, Spain

    Soraya Carrancio & Sandra Muntion

  4. Department of Cell Biology, Facultad de Medicina, Universidad de Salamanca, Salamanca, Spain

    Jesus García-Briñon

  5. Centro en Red de Terapia Celular y Medicina Regenerativa de Castilla y León, Red Nacional de Terapia Celular (TerCel), Salamanca, Spain

    Juan F. Blanco, Fermin M. Sánchez-Guijo, Soraya Carrancio, Sandra Muntion, Jesus García-Briñon & Maria-Consuelo del Cañizo

Authors
  1. Juan F. Blanco
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  2. Fermin M. Sánchez-Guijo
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  3. Soraya Carrancio
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  5. Jesus García-Briñon
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  6. Maria-Consuelo del Cañizo
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Correspondence to Juan F. Blanco.

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Blanco, J.F., Sánchez-Guijo, F.M., Carrancio, S. et al. Titanium and tantalum as mesenchymal stem cell scaffolds for spinal fusion: an in vitro comparative study. Eur Spine J 20 (Suppl 3), 353 (2011). https://doi.org/10.1007/s00586-011-1901-8

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  • DOI: https://doi.org/10.1007/s00586-011-1901-8

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