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Effects of thiolic antioxidants on in vitro mouse peritoneal macrophage functions

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Abstract

Since leukocytes have the potential to produce oxygen free radicals that damage biomolecules, we have evaluated the influence of thiolic antioxidants (glutathione, N-acetylcysteine and thioproline) on selected hematoimmunological characteristics. We used peritoneal leukocytes incubated at 0.5, 1.0 and 5.0 mM concentration of antioxidants. Following thiol treatment, we found a stimulation of phagocytic activity, and markedly decreased incidence of programmed cell death of peritoneal leukocytes, both basal and induced by H2O2. Low concentrations of thiolic compounds also increased the activity of catalase, glutathione peroxidase and glutathione reductase. On the contrary, only the highest concentration of N-acetylcysteine and thioproline reduced superoxide dismutase activity. Tested antioxidants can lead to a decrease of the oxidative stress, and could provide a nutritional benefit for evaluated immunological characteristics.

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Acknowledgements

This work was supported by MCYT (BFI 2001-1218, Spain; to M.F.), CM (08.5/0061/2001.1, Spain; to M.F.) and MSMT (1894/03 and 1110/04, Czech Republic; to J.B.) grants.

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Authors and Affiliations

  1. Department of Clinical Studies, Faculty of Health and Social Care, University of South Bohemia, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic

    M. Pomaki & J. Berger

  2. Department of Animal Physiology, Faculty of Biology, Complutense University, Spain

    M. Pomaki, J. Mota & M. De La. Fuente

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  1. M. Pomaki
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  2. J. Mota
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  3. M. De La. Fuente
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  4. J. Berger
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Correspondence to J. Berger.

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Pomaki, M., Mota, J., Fuente, M.D.L. et al. Effects of thiolic antioxidants on in vitro mouse peritoneal macrophage functions. Comp Clin Path 13, 176–181 (2005). https://doi.org/10.1007/s00580-005-0545-7

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  • DOI: https://doi.org/10.1007/s00580-005-0545-7

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