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Analysis of hepatitis A virus protein 2B in sera of hepatitis A of various severities

  • Liver, Pancreas, and Biliary Tract
  • Published:
Journal of Gastroenterology Aims and scope Submit manuscript

An Erratum to this article was published on 29 February 2008

Abstract

Background

In our recent study of the full-length hepatitis A virus (HAV) genome from some patients with fulminant hepatitis and acute hepatitis, possible associations were suggested between the severity of hepatitis A and the amino acid substitutions in the nonstructural protein 2B. We therefore analyzed HAV 2B from many patients with various clinical disease severities.

Methods

Serum samples from 30 Japanese patients with sporadic hepatitis A from five widely separated regions of Japan, comprising nine patients with fulminant hepatitis (FH), six with severe acute hepatitis (AHs), and 15 with acute hepatitis (AH), were examined for HAV RNA. The entire sequences of HAV 2B were analyzed.

Results

Compared with the sequence of the wild-type HAV strain GBM, nucleotide sequences of 2B had homology of 94.5 ± 1.0% in FH, 95.2 ± 1.2% in AHs, and 95.1 ± 1.8% in AH. Deduced amino acid sequences had homology of 97.5 ± 2.1% in FH, 97.9 ± 2.4% in AHs, and 98.5 ± 1.3% in AH. Differences were not statistically significant among the three groups. The average number of amino acid mutations between amino acids 100 and 200 was 5.0 ± 5.2 per case in FH, 4.0 ± 6.0 in AHs, and 1.9 ± 2.9 in AH. The differences between FH and AH, AHs and AH, and between severe cases (FH and AHs) and nonsevere cases (AH) were not statistically significant (P = 0.13, P = 0.45, and P = 0.10, respectively).

Conclusions

There were no obvious differences in the sequences among FH, AHs, and AH throughout the 2B region, but there seemed to be more mutations in the strains obtained from FH and AHs patients than in those obtained from AH patients in the central part of HAV 2B.

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Authors and Affiliations

  1. Department of Medicine and Clinical Oncology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan

    Keiichi Fujiwara, Osamu Yokosuka & Fumio Imazeki

  2. Department of Internal Medicine, Yokohama Higashi National Hospital, Yokohama, Kanagawa, Japan

    Makoto Miki

  3. First Department of Internal Medicine, Iwate Medical University, Morioka, Iwate, Japan

    Kazuyuki Suzuki

  4. Department of Gastroenterology and Hepatology, Yamaguchi University School of Medicine, Ube, Yamaguchi, Japan

    Kiwamu Okita

  5. Second Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Nagano, Japan

    Eiji Tanaka

  6. Department of Gastroenterology, Faculty of Medicine, University of Tokyo, Tokyo, Japan

    Masao Omata

Authors
  1. Keiichi Fujiwara
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  2. Osamu Yokosuka
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  3. Fumio Imazeki
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  4. Makoto Miki
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  5. Kazuyuki Suzuki
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  6. Kiwamu Okita
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  7. Eiji Tanaka
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  8. Masao Omata
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Additional information

An erratum to this article can be found online at http://dx.doi.org/10.1007/s00535-007-2154-5.

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Fujiwara, K., Yokosuka, O., Imazeki, F. et al. Analysis of hepatitis A virus protein 2B in sera of hepatitis A of various severities. J Gastroenterol 42, 560–566 (2007). https://doi.org/10.1007/s00535-007-2039-7

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  • DOI: https://doi.org/10.1007/s00535-007-2039-7

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