Abstract
Recombinant Escherichia coli whole cells harboring Bacillus licheniformis l-arabinose isomerase (BLAI) were immobilized with alginate. The operational conditions for immobilization were optimized with response surface methodology. Optimal alginate concentration, Ca2+ concentration, and cell mass loading were 1.8% (w/v), 0.1 M, and 44.5 g L−1, respectively. The interactions between Ca2+ concentration, alginate concentration, and initial cell mass were significant. After immobilization of BLAI, cross-linking with 0.1% glutaraldehyde significantly reduced cell leakage. The half-life of immobilized whole cells was 150 days, which was 50-fold longer than that of free cells. In seven repeated batches for l-ribulose production, the productivity was as high as 56.7 g L−1 h−1 at 400 g L−1 substrate concentration. The immobilized cells retained 89% of the initial yield after 33 days of reaction. Immobilization of whole cells harboring BLAI, therefore, makes a suitable biocatalyst for the production of l-ribulose, particularly because of its high stability and low cost.
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This work was supported by the 21C Frontier Microbial Genomics and Applications Center Program, Ministry of Education, Science & Technology, Republic of Korea. This research was supported by a grant from the Korea Research Foundation (KRF-2007-314-D00073). This research was also supported by the 2009 KU Brain Pool of Konkuk University.
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Zhang, YW., Prabhu, P. & Lee, JK. Alginate immobilization of recombinant Escherichia coli whole cells harboring l-arabinose isomerase for l-ribulose production. Bioprocess Biosyst Eng 33, 741–748 (2010). https://doi.org/10.1007/s00449-009-0397-7
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DOI: https://doi.org/10.1007/s00449-009-0397-7