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Low-volume exercise training attenuates oxidative stress and neutrophils activation in older adults

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Abstract

The purpose of this study was to investigate the effects of low-volume exercise training (100 min/week) on oxidative stress and neutrophils activation markers in older adults. Twenty-eight older adults (age range 65–78 years) were assigned into control (n = 14) or exercise (n = 14) groups. The exercise program consisted of walking 30–60 min/session, 2 days each week for 12 weeks. Blood samples were taken before starting the sessions (baseline) and when they ended. Fasting plasma and serum oxidative stress and inflammatory markers were measured using commercial kits. Cell surface expression of adhesion molecules on circulating leukocytes (CD66b and CD62L) was determined using flow cytometry. The concentrations of derivatives of reactive oxygen metabolites tended to be lower than the baseline values only in the exercise group (P = 0.05). The biological antioxidant potential, thioredoxin concentrations, and glutathione peroxidase activities significantly increased only in the exercise group (P < 0.05 for all). While CD66b expression tended to decrease only in the exercise group, CD62L expression significantly increased (P < 0.05). Our findings indicate that exercise training below the current recommended level of at least 150 min/week attenuates basal oxidative stress and neutrophil activation in older adults. Thus, our findings may encourage more people to incorporate a small amount of physical activity into their lives.

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Acknowledgments

This study was supported by grants from the Ministry of Education, Culture, Sports Science and Technology of Japan; the Grant-in-Aid for the Global COE Program “Sport Science for the Promotion of Active Life” (2010–2011) and the Scientific Research (A) 23240097; the Uehara Memorial Foundation; the Nakatomi Foundation; and the Kao Corporation.

Conflict of interest

All authors declare that there is no conflict of interest.

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Authors and Affiliations

  1. Graduate School of Sport Sciences, Waseda University, Tokorozawa, Saitama, Japan

    Masaki Takahashi, Noriaki Kawanishi, Jong-Hwan Park, Harumi Hayashida & Hyun-Shik Kim

  2. Japan Society for the Promotion of Science, Tokyo, Japan

    Masaki Takahashi & Noriaki Kawanishi

  3. Faculty of Sport Sciences, Waseda University, 2-579-15 Mikajima, Tokorozawa, Saitama, 359-1192, Japan

    Masashi Miyashita, Yoshio Nakamura, Shizuo Sakamoto & Katsuhiko Suzuki

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  1. Masaki Takahashi
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  2. Masashi Miyashita
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Correspondence to Masashi Miyashita.

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Communicated by William J. Kraemer.

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Takahashi, M., Miyashita, M., Kawanishi, N. et al. Low-volume exercise training attenuates oxidative stress and neutrophils activation in older adults. Eur J Appl Physiol 113, 1117–1126 (2013). https://doi.org/10.1007/s00421-012-2531-5

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  • DOI: https://doi.org/10.1007/s00421-012-2531-5

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