Abstract
Y-Chromosomal short tandem repeat polymorphisms (Y-STRs) are widely applied in human forensic cases and population genetic studies. There is a lack of information about the Sichuan Tibetan population in the Y-STR Haplotype Reference Database (YHRD, https://yhrd.org, release 59). In this study, 502 unrelated male individuals residing in the Sichuan Province were recruited and genotyped at 29 Y-STR loci. A total of 479 haplotypes were observed, 460 (96.03%) of which were unique. The haplotype diversity (HD) and discrimination capacity (DC) for the Sichuan Tibetan population were 0.9998 and 0.9542, respectively. To reveal the genetic diversities and relationships between the Chinese Sichuan Tibetan and 29 other previously reported populations, forensic parameter analysis, multi-dimensional scaling, and phylogenetic reconstruction were performed. The results showed that the Sichuan Tibetan population was relatively isolated from other populations, suggesting that genetic proximity is in line with geographical boundaries.
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Gayden T, Bukhari A, Chennakrishnaiah S, Stojkovic O, Herrera RJ (2012) Y-chromosomal microsatellite diversity in three culturally defined regions of historical Tibet. Forensic Sci Int Genet 6(4):437–446
Rowold DJ, Gayden T, Luis JR, Alfonso-Sanchez MA, Garcia-Bertrand R, Herrera RJ (2019) Investigating the genetic diversity and affinities of historical populations of Tibet. Gene 682:81–91
Ye Y, Gao J, Fan G, Liao L, Hou Y (2015) Population genetics for 23 Y-STR loci in Tibetan in China and confirmation of DYS448 null allele. Forensic Sci Int Genet 16:e7–e10
Kayser M, Kittler R, Erler A, Hedman M, Lee AC, Mohyuddin A, Mehdi SQ, Rosser Z, Stoneking M, Jobling MA, Sajantila A, Tyler-Smith C (2004) A comprehensive survey of human Y-chromosomal microsatellites. Am J Hum Genet 74(6):1183–1197
Vermeulen M, Wollstein A, van der Gaag K, Lao O, Xue Y, Wang Q, Roewer L, Knoblauch H, Tyler-Smith C, de Knijff P, Kayser M (2009) Improving global and regional resolution of male lineage differentiation by simple single-copy Y-chromosomal short tandem repeat polymorphisms. Forensic Sci Int Genet 3(4):205–213
Purps J, Siegert S, Willuweit S, Nagy M, Alves C, Salazar R, Angustia SMT, Santos LH, Anslinger K, Bayer B, Ayub Q, Wei W, Xue Y, Tyler-Smith C, Bafalluy MB, Martínez-Jarreta B, Egyed B, Balitzki B, Tschumi S, Ballard D, Court DS, Barrantes X, Bäßler G, Wiest T, Berger B, Niederstätter H, Parson W, Davis C, Budowle B, Burri H, Borer U, Koller C, Carvalho EF, Domingues PM, Chamoun WT, Coble MD, Hill CR, Corach D, Caputo M, D’Amato ME, Davison S, Decorte R, Larmuseau MHD, Ottoni C, Rickards O, Lu D, Jiang C, Dobosz T, Jonkisz A, Frank WE, Furac I, Gehrig C, Castella V, Grskovic B, Haas C, Wobst J, Hadzic G, Drobnic K, Honda K, Hou Y, Zhou D, Li Y, Hu S, Chen S, Immel UD, Lessig R, Jakovski Z, Ilievska T, Klann AE, García CC, de Knijff P, Kraaijenbrink T, Kondili A, Miniati P, Vouropoulou M, Kovacevic L, Marjanovic D, Lindner I, Mansour I, al-Azem M, Andari AE, Marino M, Furfuro S, Locarno L, Martín P, Luque GM, Alonso A, Miranda LS, Moreira H, Mizuno N, Iwashima Y, Neto RSM, Nogueira TLS, Silva R, Nastainczyk-Wulf M, Edelmann J, Kohl M, Nie S, Wang X, Cheng B, Núñez C, Pancorbo MM, Olofsson JK, Morling N, Onofri V, Tagliabracci A, Pamjav H, Volgyi A, Barany G, Pawlowski R, Maciejewska A, Pelotti S, Pepinski W, Abreu-Glowacka M, Phillips C, Cárdenas J, Rey-Gonzalez D, Salas A, Brisighelli F, Capelli C, Toscanini U, Piccinini A, Piglionica M, Baldassarra SL, Ploski R, Konarzewska M, Jastrzebska E, Robino C, Sajantila A, Palo JU, Guevara E, Salvador J, Ungria MCD, Rodriguez JJR, Schmidt U, Schlauderer N, Saukko P, Schneider PM, Sirker M, Shin KJ, Oh YN, Skitsa I, Ampati A, Smith TG, Calvit LS, Stenzl V, Capal T, Tillmar A, Nilsson H, Turrina S, de Leo D, Verzeletti A, Cortellini V, Wetton JH, Gwynne GM, Jobling MA, Whittle MR, Sumita DR, Wolańska-Nowak P, Yong RYY, Krawczak M, Nothnagel M, Roewer L (2014) A global analysis of Y-chromosomal haplotype diversity for 23 STR loci. Forensic Sci Int Genet 12:12–23
Budowle B, Ge J, Low J, Lai C, Yee WH, Law G, Tan WF, Chang YM, Perumal R, Keat PY, Mizuno N, Kasai K, Sekiguchi K, Chakraborty R (2009) The effects of Asian population substructure on Y STR forensic analyses. Leg Med (Tokyo) 11(2):64–69
Walsh PS, Metzger DA, Higuchi R (1991) Chelex-100 as a medium for simple extraction of DNA for Pcr-based typing from forensic material. Biotechniques 10(4):506–513
Nei M, Tajima F (1981) DNA polymorphism detectable by restriction endonucleases. Genetics 97(1):145–163
Excoffier L, Lischer HE (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10(3):564–567
Yao HB, Wang CC, Wang J, Tao X, Shang L, Wen SQ, du Q, Deng Q, Xu B, Huang Y, Wang HD, Li S, Bin Cong, Ma L, Jin L, Krause J, Li H (2017) Genetic structure of Tibetan populations in Gansu revealed by forensic STR loci. Sci Rep 7:41195
Zou X, Wang Z, He G, Wang M, Su Y, Liu J, Chen P, Wang S, Gao B, Li Z, Hou Y (2018) Population genetic diversity and phylogenetic characteristics for high-altitude adaptive Kham Tibetan revealed by DNATyperTM 19 amplification system. Front Genet 9:630
Parson W, Roewer L (2010) Publication of population data of linearly inherited DNA markers in the International Journal of Legal Medicine. Int J Legal Med 124:505–509
Poetsch M, Bajanowski T, Pfeiffer H (2012) The publication of population genetic data in the International Journal of Legal Medicine: guidelines. Int J Legal Med 126:489–490
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The authors thank the voluntary participants in this study.
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This study was supported by the National Natural Science Foundation of China (NSFC, No. 81772030, and 81701866), the Postdoctoral Fund of Sichuan University (No.2017SCU12049), and the Outstanding Youth Fund of Sichuan University (No. 2016SCU04A07).
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Song, F., Xie, M., Xie, B. et al. Genetic diversity and phylogenetic analysis of 29 Y-STR loci in the Tibetan population from Sichuan Province, Southwest China. Int J Legal Med 134, 513–516 (2020). https://doi.org/10.1007/s00414-019-02043-y
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DOI: https://doi.org/10.1007/s00414-019-02043-y