Skip to main content
SpringerLink
Log in

Assignment of 19 porcine type I loci by somatic cell hybrid analysis detects new regions of conserved synteny between human and pig

  • Original Contributions
  • Published:
Mammalian Genome Aims and scope Submit manuscript

Abstract

Nineteen so-called type I-loci, including ACO2, ADRA2, CAST, CCK, CHAT, IGKC, IGLV, IL4, IL6, INHA, L1F, MX1, PTH, RBP2, TCRA, TCRB, TGFB2, TGFB3, and UOX have been mapped in the pig with an informative somatic cell hybrid panel. By analyzing these new assignments in the knowledge of heterologous chromosome painting (Zoo-FISH) data for the porcine genome, it is possible to predict subchromosomal locations for most of these loci. Previously defined regions of conserved synteny were confirmed, and the extent of six of these regions was refined. These improvements in the porcine gene map facilitate the transfer of gene mapping data from “map-rich” species such as humans and mice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Andersson, L., Haley, C.S., Ellegren, H., Knott, S.A., Johansson, M., Andersson, K., Andersson-Eklund, L., Edfors-Lilja, I., Fredholm, M., Hansson, I., Håkansson, J., Lundström, K. (1994). Genetic mapping of quantitative trait loci for growth and fatness in pigs. Science 263, 1771–1774.

    Article  PubMed  CAS  Google Scholar 

  • Archibald, A.L., Brown, J.F., Couperwhite, S., McQueen, H.A., Nicholson, D., Coppieters, W., Van de Weghe, A., Stratil, A., Wintero, A.K., Fredholm, M., Larsen, N.J., Nielsen, V.H., Milan, D., Woloszyn, N., Robic, A., Dalens, M., Riquet, J., Gellin, J., Caritez, J.-C., Burgaud, G., Ollivier, L., Bidanel, J.-P., Vaiman, N., Renard, C., Geldermann, H., Davoli, R., Ruyter, D., Verstege, E.J.M., Groenen, M.A.M., Davies, W., Hoyheim, B., Keiserud, A., Andersson, L., Ellegren, H., Johansson, M., Marklund, L., Miller, J.R., Anderson Dear, D.V., Signer, E., Jeffreys, A.J., Moran, C., Le Tissier, P., Muladno, Rothschild, M.F., Tuggle, C.K., Vaske, D., Helm, J., Liu, H.-C., Rahman, A., Yu, T.-P., Larson, R.G., Schmitz, C.B. (1995). The PiGMaP consortium linkage map of the pig (Sus scrofa). Mamm. Genome 6, 157–175.

    Article  PubMed  CAS  Google Scholar 

  • Brown, J.F., Hardge, T., Rettenberger, G., Archibald, A.L. (1994). Four new porcine polymorphic microsatellite loci (SOO32, SOO34, SOO36 & SOO37). Anim. Genet. 25, 365.

    PubMed  CAS  Google Scholar 

  • Chevalet, C., Corpet, F. (1986). Statistical decision rules concerning synteny or independence between markers. Cytogenet. Cell Genet. 43, 132–139.

    Article  PubMed  CAS  Google Scholar 

  • Collins, F.S. (1992). Positional cloning: Let’s not call it reverse anymore. Nature Genet. 1, 3–6.

    Article  PubMed  CAS  Google Scholar 

  • Copeland, N.G., Jenkins, N.A., Gilbert, D.J., Eppig, J.T., Maltais, L.J., Miller, J.C., Dietrich, W.F., Weaver, A., Lincoln, S.E., Steen, R.G., Stein, L.D., Nadeau, J.H., Lander, E.S. (1993). A genetic linkage map of the mouse: current applications and future prospects. Science 262, 57–66.

    Article  PubMed  CAS  Google Scholar 

  • Coppieters, W., Van de Weghe, A., Peelman, L., Depicker, A., Van Zeveren, A., Bouquet, Y. (1993). Characterization of porcine polymorphic microsatellite loci. Animal Genet. 24, 163–170.

    PubMed  CAS  Google Scholar 

  • Dietrich, W.F., Miller J.C., Steen, R.G., Merchant, M., Damron, D., Nahf, R., Gross, A., Joyce, D.C., Wessel, M., Dredge, R.D., Marquis, A., Stein, L.D., Goodman, N., Page, D.C., Lander, E.S. (1994). A genetic map of the mouse with 4006 simple sequence length polymorphisms. Nature Genet. 7, 220–245.

    Article  PubMed  CAS  Google Scholar 

  • Dolf, G., Stranzinger, G. (1986). Pig gene mapping. Assignment of the genes for mannose phosphate isomerase (MPI) and nucleoside phosphorylase (NP) to chromosome no. 7. Genet. Sel. Evol. 18, 375–384.

    Article  CAS  Google Scholar 

  • Ellegren, H. (1993). Abundant (A)n(T)n mononucleotide repeats in the pig genome: linkage mapping of the porcine APOB, FSA, ACOX12, PEPN and RLN loci. Animal Genet. 24, 367–372.

    PubMed  CAS  Google Scholar 

  • Ellegren, H., Johansson, M., Chowdhary, B.P., Marklund, S., Ruyter, D., Marklund, L., Nielsen, P.B., Edfors-Lilja, I., Gustavsson, I., Juneja, R.K., Andersson, L. (1993). Assignment of 20 microsatellite markers to the porcine linkage map. Genomics 16, 431–439.

    Article  PubMed  CAS  Google Scholar 

  • Fasman, K.H., Cuticchia, A.J., Kingsbury, D.T. (1994). The Human Genome Data Base anno 1994. Nucleic Acids Res. 22, 3462–3469.

    Article  PubMed  CAS  Google Scholar 

  • Fredholm, M., Wintero, A.K., Christensen, K., Kristensen, B., Nielsen, P.B., Davies, W., Archibald, A. (1993). Characterization of 24 porcine (dA-dCn-(dT-dG)n microsatellites, genotyping of unrelated animals from our breeds and linkage studies. Mamm. Genome 4, 187–192.

    Article  PubMed  CAS  Google Scholar 

  • Haley, C.S., Archibald, A.L. (1992). Porcine genome analysis. In Genome Analysis, Vol. 4: Strategies for Physical Mapping, KE Davies, SM Tilghman, eds. (Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press), pp. 99–129.

    Google Scholar 

  • Human Gene Mapping 11 (1991). Cytogenet. Cell Genet. 58, 1–2200.

    Article  Google Scholar 

  • Johansson, M., Ellegren, H., Andersson, L. (1995). Comparative mapping reveals extensive linkage conservation—but with gene order rearrangements—between the pig and the human genomes. Genomics 25, 682–690.

    Article  PubMed  CAS  Google Scholar 

  • Leong, M., Lin, C.C., Ruth, R. (1983). Assignment of Superoxide desmutase (SOD1) gene to chromosome Nr. 9 of domestic pig. Can. J. Genet. Cyto. 25, 233–238.

    CAS  Google Scholar 

  • Levan, G., Szpirer, J., Szpirer, C., Klinga, K., Hanson, C., Islam, M.Q. (1991). The gene map of the Norway rat (Rattus norvegicus) and comparative mapping with mouse and man. Genomics 10, 699–718.

    Article  PubMed  CAS  Google Scholar 

  • McKusick, V.A. (1992). Mendelian inheritance in man: the human gene map, 10th ed. (Baltimore: Johns Hopkins University Press).

    Google Scholar 

  • McQueen, H.A., Yerle, M., Archibald, A.L. (1994). Anchorage of an unassigned linkage group to pig chromosome 10 using P1 clones. Mamm. Genome 5, 646–648.

    Article  PubMed  CAS  Google Scholar 

  • Mitelmann, F., Kaneko, J., Trent, J. (1991). Report of the committee on chromosome changes in neoplasia. Cytogenet. Cell Genet 58, 1053–1079.

    Article  Google Scholar 

  • Nadeau, J.H. (1989). Maps of linkage and synteny homologies between mouse and man. Trends Genet. 5, 82–86.

    Article  PubMed  CAS  Google Scholar 

  • O’Brien, S.J., Womack, J.E., Lyons, L.A., Moore, K.J., Jenkins, N.A., Copeland, N.G. (1993). Anchored reference loci for comparative mapping in mammals. Nature Genet. 3, 103–112.

    Article  PubMed  Google Scholar 

  • Rettenberger, G., Fries, R., Enge, W., Scheit, K.H., Dolf, G., Hameister, H. (1994a). Establishment of a partially informative porcine somatic cell hybrid panel and assignment of the loci for transition protein 2 (TNP2) and protamine 1 (PRM1) to chromosome 3 and polyubiquitin (UBC) to chromosome 14. Genomics 21, 558–566.

    Article  PubMed  CAS  Google Scholar 

  • Rettenberger, G., Burkhardt, E., Adham, I.M., Engel, W., Fries, R., Klett, C., Hameister, H. (1994b). Assignment of the Leydig-insulin like hormone to porcine chromosome 2ql2-ql3 by somatic cell hybrid analysis and fluorescence in situ hybridization. Mamm. Genome 5, 307–309.

    Article  PubMed  CAS  Google Scholar 

  • Rettenberger, G., Fredholm, M., Fries, R. (1994c) Chromosomal assignment of porcine microsatellites by use of a somatic cell hybrid mapping panel. Anim. Genet. 25, 343–345.

    Article  PubMed  CAS  Google Scholar 

  • Rettenberger, G., Klett, Ch., Zechner, U., Kunz, J., Vogel, W., Hameister, H. (1995). Visualization of the conservation of synteny between humans and pigs by heterologous chromosomal painting. Genomics 26, 372–378.

    Article  PubMed  CAS  Google Scholar 

  • Rettenberger, G., Klett, Ch., Zechner, U., Bruch, J., Just, W., Vogel, W., Hameister, H. (1996). ZOO-FISH analysis: the cat and also the human karyotypes closely resemble the putative ancestral mammalian karyotype. Submitted.

  • Roth, H.R., Dolf, G., Stranzinger, G. (1987). Gene assignment by means of somatic cell hybrids, a new method for the analysis of data. Theor. Appl. Genet. 74, 42–48.

    Article  Google Scholar 

  • Scherthan, H., Cremer, T., Arnason, U., Weier, H.-U., Lima-de-Faria, A., Frönicke, L. (1994). Comparative chromosome painting discloses homologous segments in distantly related mammals. Nature Genet. 6, 342–347.

    Article  PubMed  CAS  Google Scholar 

  • Searle, A.G., Peters, J., Lyon, M.F., Hall, J.G., Evans, E.P., Edwards, J.H., Buckle, V.J. (1989). Chromosome maps of man and mouse. IV. Ann. Hum. Genet. 53, 89–140.

    Article  PubMed  CAS  Google Scholar 

  • Solinas-Toldo, S., Lengauer, C., Fries, R. (1995). Comparative genome map of human and cattle. Genomics 27, 489–496.

    Article  PubMed  CAS  Google Scholar 

  • Threadgill, D.W., Womack, J.E. (1990). Syntenic conservation between humans and cattle. I. Human chromosome 9. Genomics 8, 22–28.

    Article  PubMed  CAS  Google Scholar 

  • Wienberg, J., Stanyon, R., Jauch, A., Cremer, T. (1992). Homologies in human and Macaca fuscata chromosomes revealed by in situ suppression hybridization with human chromosome specific DNA libraries. Chromosoma 101, 265–270.

    Article  PubMed  CAS  Google Scholar 

  • Yeldandi, A.V., Patel, Y.D., Liao, M., Kao, F.T., Rao, M.S., Reddy, J.K., Le Beau, M.M. (1992). Localization of the human urate oxidase gene (UOX) to 1p22. Cytogenet. Cell Genet. 61, 121–122.

    Article  PubMed  CAS  Google Scholar 

  • Yerle, M., Lahbib-Mansais, Y., Mellink, C., Goureau, A., Pinton, P., Echard, G., Gellin, J., Zijlstra, C., De Haan, N., Bosma, A.A., Chowdhary, B.P., Gu, F., Gustavsson, I., Thomsen, P.D., Christensen, K., Rettenberger, G., Hameister, H., Schmitz, A., Chaput, B., Frelat, G. (1995). The PiGMaP consortium cytogenetic map of the domestic pig (Sus scrofa domestica). Mamm. Genome 6, 176–186.

    Article  PubMed  CAS  Google Scholar 

  • Yunis, J.J., Prakash, O. (1982). The origin of man: a chromosomal pictorial legacy. Science 215, 1525–1530.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical Genetics, University of Ulm, D-89069, Ulm, Germany

    G. Rettenberger, J. Bruch & H. Hameister

  2. Department of Animal Science, Swiss Federal Institute of Technology, CH-8092, Zürich, Switzerland

    R. Fries

  3. Roslin Institute (Edinburgh), EH25 9PS, Midlothian, Scotland, UK

    A. L. Archibald

Authors
  1. G. Rettenberger
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Bruch
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Fries
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. L. Archibald
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H. Hameister
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rettenberger, G., Bruch, J., Fries, R. et al. Assignment of 19 porcine type I loci by somatic cell hybrid analysis detects new regions of conserved synteny between human and pig. Mammalian Genome 7, 275–279 (1996). https://doi.org/10.1007/s003359900082

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s003359900082

Keywords

Search

Navigation