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Molecular characterization of a novel fission yeast gene spUAP2 that interacts with the splicing factor spU2AF59

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Abstract

A protein essential for pre-mRNA splicing, the U2 auxiliary factor (U2AF), is composed of a large and small subunit. Previously we cloned and characterized both subunits, spU2AF59 and spU2AF23, from fission yeast. We now report a novel U2AF-associated-protein, spUAP2, which interacts with both subunits. SpUAP2 contains a classical and a degenerate RNA recognition motif (RRM), both of which are required for interaction with spU2AF59. Interaction also requires the arginine/serine-rich region and the first RRM of spU2AF59. A null allele of the gene for spUAP2 is lethal.

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Authors and Affiliations

  1. Department of Pharmacology and Molecular Biology, Finch University of Health Sciences, The Chicago Medical School, 3333 Green Bay Road, North Chicago, IL 60064, USA Fax: +1-847-578-3268; e-mail: potashkj@mis.finchcms.edu, , , , , , US

    R. McKinney, Kelly Wentz-Hunter & J. Potashkin

  2. Institut für Genetik, Biozentrum, Technische Universität Braunschweig, Spielmannstrasse 7, D-38106 Braunschweig, Germany, , , , , , DE

    Henning Schmidt

Authors
  1. R. McKinney
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  2. Kelly Wentz-Hunter
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  3. Henning Schmidt
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  4. J. Potashkin
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Received: 23 May / Accepted: 6 August 1997

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McKinney, R., Wentz-Hunter, K., Schmidt, H. et al. Molecular characterization of a novel fission yeast gene spUAP2 that interacts with the splicing factor spU2AF59. Curr Genet 32, 323–330 (1997). https://doi.org/10.1007/s002940050284

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  • DOI: https://doi.org/10.1007/s002940050284

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