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Ligninolytic Enzymes of Endospore-Forming Bacillus aryabhattai BA03

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In this work we investigated the enzymes produced by Bacillus aryabhattai BA03, particularly in those involved in ligninolytic activities such as laccases (Lac), lignin peroxidases (LiP) and Mn-dependent peroxidase (MnD-P). In this way, the maximal production of Lac (0.069 ± 0.000 U/mL) was obtained at pH 9, and 37 ºC after 72 h. LiP expressed the highest activity at 96 h in acid medium at 37 ºC (0.741 ± 0.029 U/mL). Meanwhile, the strain produced MnD-P (1.052 ± 0.001 U/mL) at the highest temperature assayed (44 ºC) and pH 7 at 72 h. In addition, this microorganism produced resistant endospores able to germinate after the sterilization program (121 ºC, 15 min) showing a high enzymatic activity. Using the heat-treated culture as inoculum, the percentage of decolorization of 150 mg/L of Coomassie Brillant Blue reached 89.42 ± 0.11% in only 24 h. These results open the use of these enzymes and endospores in bioremediation processes carried out under different temperatures and pH values.

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Acknowledgments

We are grateful to the Spanish Ministry of Economy and Competitiveness for the financial support given for this study (Project CTQ2015-71436-C2-1-R), which has partial financial support from the FEDER funds of the European Union, and to the “Consellería de Cultura, Educación e Ordenación Universitaria” of Xunta de Galicia for Alicia Pérez Paz’s postdoctoral fellowship ED481B 2018/073, and FAPESP (São Paulo Research Foundation) for the process n. 2018/25511-1. This study forms part of the activities of the Group with Potential for Growth (ED431B 2018/54-GPC), the Xunta de Galicia (Spain).

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  1. Industrial Biotechnology and Environmental Engineering Group “BiotecnIA”, Chemical Engineering Department, University of Vigo (Campus Ourense), As Lagoas s/n, 32004, Ourense, Spain

    Alicia Paz, Iván Costa-Trigo & José Manuel Domínguez

  2. Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Lineu Prestes 580, Bl 16, São Paulo, 05508-900, Brazil

    Ricardo Pinheiro de Souza Oliveira

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  1. Alicia Paz
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Paz, A., Costa-Trigo, I., Oliveira, R.P.S. et al. Ligninolytic Enzymes of Endospore-Forming Bacillus aryabhattai BA03. Curr Microbiol 77, 702–709 (2020). https://doi.org/10.1007/s00284-019-01856-9

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