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Aeromonas lusitana sp. nov., Isolated from Untreated Water and Vegetables

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Abstract

During previous studies to evaluate the phylogenetic diversity of Aeromonas from untreated waters and vegetables intended for human consumption, a group of isolates formed a unique gyrB phylogenetic cluster, separated from those of all other species described so far. A subsequent extensive phenotypic characterization, DNA–DNA hybridization, 16S rRNA gene sequencing, multi-locus phylogenetic analysis of the concatenated sequence of seven housekeeping genes (gyrB, rpoD, recA, dnaJ, gyrA, dnaX, and atpD; 4705 bp), and ERIC-PCR, were performed in an attempt to ascertain the taxonomy position of these isolates. This polyphasic approach confirmed that they belonged to a novel species of the genus Aeromonas, for which the name Aeromonas lusitana sp. nov. is proposed, with strain A.11/6T (=DSMZ 24095T, =CECT 7828T) as the type strain.

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Acknowledgments

Part of the research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under Grant agreement No.: 311846. AMM wish to acknowledge the support provided by the laboratory GPSTM of Genetic Analysis Strategies SL. In memory of Prof. Antonio Correia, sadly passed away on January 5, 2016.

Author information

Authors and Affiliations

  1. Area de Microbiología, EPSO, Universidad Miguel Hernández, 03312, Orihuela, Alicante, Spain

    Antonio Martínez-Murcia

  2. Genetic PCR Solutions TM, CEEI, Elche, Alicante, Spain

    Antonio Martínez-Murcia & Aaron Navarro

  3. Unitat de Microbiologia, Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina i Ciències de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain

    Roxana Beaz-Hidalgo & Mª. José Figueras

  4. Department of Biology & CESAM, University of Aveiro, 3810‐193, Aveiro, Portugal

    Mª. João Carvalho & Antonio Correia

  5. School of Pathology and Laboratory Medicine, The University of Western Australia Crawley, Crawley, WA, Australia

    Max Aravena-Román

  6. Department of Veterinary Sciences, CECAV-University of Trás-os-Montes e Alto Douro, Vila Real, Portugal

    Mª. José Saavedra

Authors
  1. Antonio Martínez-Murcia
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  2. Roxana Beaz-Hidalgo
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  3. Aaron Navarro
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  4. Mª. João Carvalho
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  6. Antonio Correia
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  7. Mª. José Figueras
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  8. Mª. José Saavedra
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Corresponding author

Correspondence to Antonio Martínez-Murcia.

Additional information

The GenBank accession numbers of Aeromonas lusitana genes sequences are: HQ436039 (16S rRNA), HQ442672- HQ442676 (gyrB), HQ442765-HQ442769 (rpoD), HQ442900-HQ442904 (recA), HQ443031- HQ443035 (dnaJ), HQ443125- HQ4431259 (gyrA), HQ442514- HQ442518 (dnaX), and HQ442631-HQ442635 (atpD).

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Martínez-Murcia, A., Beaz-Hidalgo, R., Navarro, A. et al. Aeromonas lusitana sp. nov., Isolated from Untreated Water and Vegetables. Curr Microbiol 72, 795–803 (2016). https://doi.org/10.1007/s00284-016-0997-9

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