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Characterization of Antimicrobial Activity of the Lysosomes Isolated from Saccharomyces cerevisiae

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Abstract

The antimicrobial activity of lysosomes, a cell organelle, against a range of test microorganisms was examined in this study. The lysosomes isolated from Saccharomyces cerevisiae showed antimicrobial activity to Escherichia coli that positively correlated with the pH of the phosphate buffer as a dissolving solvent. The lysosomes from S. cerevisiae exhibited optimal activity at a concentration of 40%, at pH 4.0 of phosphate buffer, and at broad range temperature, except of over 50°C. It was also found that the lysosomes have antimicrobial activity against seven different microorganisms including E. coli. In addition, S. cerevisiae were exposed by a treatment with H2O2 and lysosomes were isolated from H2O2 exposed S. cerevisiae. We found that fluorescent intensities of each isolated lysosomes were increased depending on the increment of treated H2O2 concentration, and the lysosomes from 20 mM H2O2 treated S. cerevisiae showed higher antimicrobial activity than those from normal S. cerevisiae. Therefore, it suggests that lysosomes isolated from S. cerevisiae can be used as an antimicrobial agent. In addition, lysosomes activated by H2O2 enhanced its antimicrobial activity.

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Acknowledgments

This research was financially supported by the Ministry of Knowledge Economy and Korea Industrial Technology Foundation (KOTEF) through the Human Resource Training Project for Regional Innovation. The authors are grateful for their support.

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Authors and Affiliations

  1. Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Duckjin-dong, Jeonju, 561-756, South Korea

    Jihee Yoon & Jiho Min

  2. School of Life Science, Chungbuk National University, 410 Sungbong-Ro, Heungduk-Gu, Cheongju, 361-763, South Korea

    Jae-Min Park & Yang-Hoon Kim

  3. Bioresource Inc., 484-3, Juam-myeon, Suncheon, Jeollanam-do, 540-843, South Korea

    Seung-Ki Jung & Keun-Young Kim

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  1. Jihee Yoon
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  2. Jae-Min Park
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  3. Seung-Ki Jung
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  4. Keun-Young Kim
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  5. Yang-Hoon Kim
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Correspondence to Yang-Hoon Kim or Jiho Min.

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Yoon, J., Park, JM., Jung, SK. et al. Characterization of Antimicrobial Activity of the Lysosomes Isolated from Saccharomyces cerevisiae . Curr Microbiol 59, 48–52 (2009). https://doi.org/10.1007/s00284-009-9392-0

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  • DOI: https://doi.org/10.1007/s00284-009-9392-0

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