Abstract
In previous work, we studied the anaerobic biodegradation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) by a methanogenic mixed culture that biodegrades RDX by using H2 as the sole electron donor. Strain HAAP-1 was isolated after enriching for the homoacetogens in a mineral medium containing RDX and an H2-CO2 (80:20) headspace. Strain HAAP-1 degraded 29.0 μM RDX in <14 days and formed 13.0 mM acetate when grown in a mineral medium with an H2-CO2 headspace. Methylenedinitramine was observed as a transient intermediate, indicating ring cleavage had occurred. In live cultures containing an N2-CO2 headspace, RDX was not degraded, and no acetate was formed. The 16S rRNA gene sequence for strain HAAP-1, consisting of 1485 base pairs, had a 99.2% and 99.1% sequence similarity to Acetobacterium malicum and A. wieringae, respectively. This is the first report of RDX degradation by a homoacetogen growing autotrophically and extends the number of genera known to carry out this transformation.
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Adrian, ., Arnett, . Anaerobic Biodegradation of Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) by Acetobacterium malicum Strain HAAP-1 Isolated from a Methanogenic Mixed Culture. Curr Microbiol 48, 332–340 (2004). https://doi.org/10.1007/s00284-003-4156-8
Issue Date:
DOI: https://doi.org/10.1007/s00284-003-4156-8