Abstract
Osteopoikilosis and the Buschke-Ollendorff syndrome are skeletal dysplasias with hyperostotic lesions in the long bones. These disorders are caused by heterozygous loss-of-function mutations in the LEMD3 gene. LEMD3 codes for a protein of the inner nuclear membrane that, through interaction with R-SMADs, antagonizes the BMP and TGFβ1 pathway. It is suggested that the hyperostotic lesions in these disorders are caused by enhanced BMP and TGFβ1 signaling. The exact mechanism by which mutations in the LEMD3 gene lead to these bone lesions has not yet been unraveled precisely. To further assess this, an Lemd3 gene–trapped mouse was created in a gene-trapping program by Baygenomics. To investigate whether the heterozygous gene-trapped mouse is a good model for osteopoikilosis in humans, we studied these mice radiologically with high-resolution micro-computed tomography (microCT) and histologically. X-ray images were evaluated by a trained radiologist, but no typical osteopoikilosis lesions could be recognized. On all microCT reconstructed images a 3D cortical and trabecular quantitative analysis was performed, investigating different histomorphometric parameters ranging from percent bone volume, bone surface/volume ratio over trabecular thickness, separation, number, and pattern factor to structure model index and fractal dimension. No significant differences were found after a t-test statistical analysis. Also, histological analysis did not reveal lesions typical for osteopoikilosis. We conclude that the heterozygous Lemd3 gene–trapped mouse is not a good model to study osteopoikilosis and the Buschke-Ollendorff syndrome.
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Acknowledgements
Heterozygous Lemd3 +/GT mice were kindly provided by S. G. Young (UCLA, Los Angeles). This study was supported by the Research Foundation–Flanders (Belgium, grant 3G027907), the Ghent University, the Sixth Framework of the European Commission (EuroGrow project LSHM-CT-2007-037471), and the Institute for Broad Band Technology. J. H. is a postdoctoral fellow and G. M. is a senior clinical investigator of the Research Foundation–Flanders.
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Dheedene, A., Deleye, S., Hellemans, J. et al. The Heterozygous Lemd3 +/GT Mouse Is Not a Murine Model for Osteopoikilosis in Humans. Calcif Tissue Int 85, 546–551 (2009). https://doi.org/10.1007/s00223-009-9305-z
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DOI: https://doi.org/10.1007/s00223-009-9305-z