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Supramolecular immobilization of glucose oxidase on gold coated with cyclodextrin-modified cysteamine core PAMAM G-4 dendron/Pt nanoparticles for mediatorless biosensor design

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Abstract

Cysteamine core polyamidoamine G-4 dendron branched with β-cyclodextrins was chemisorbed on the surface of Au electrodes and further coated with Pt nanoparticles. Adamantane-modified glucose oxidase was subsequently immobilized on the nanostructured electrode surface by supramolecular association. This enzyme electrode was used to construct a reagentless amperometric biosensor for glucose, making use of the electrochemical oxidation of H2O2 generated in the enzyme reaction. The amperometric response of the biosensor was rapid (6 s) and a linear function of glucose concentration between 5 and 705 μmol L−1. The biosensor had a low detection limit of 2.0 μmol L−1, sensitivity of 197 mA mol−1 L cm−2, and retained 94 % of its initial response after storage for nine days at 4 °C.

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References

  1. Newman JD, Setford SJ (2006) Enzymatic biosensors. Mol Biotechnol 32:249–268

    Article  CAS  Google Scholar 

  2. North SH, Lock EH, Taitt CR, Walton SG (2010) Critical aspects of biointerface design and their impact on biosensor development. Anal Bioanal Chem 397:925–933

    Article  CAS  Google Scholar 

  3. Li H, Liu S, Dai Z, Bao J, Yang X (2009) Applications of nanomaterials in electrochemical enzyme biosensors. Sensors 9:8547–856

    Article  CAS  Google Scholar 

  4. Guo S, Dong S (2009) Biomolecule-nanoparticle hybrids for electrochemical biosensors. Trends Anal Chem 28:96–109

    Article  CAS  Google Scholar 

  5. Pingarrón JM, Yáñez-Sedeño P, González-Cortés A (2008) Gold nanoparticle-based electrochemical biosensors. Electrochim Acta 53:5848–5866

    Article  Google Scholar 

  6. Shao Y, Wang J, Wu H, Liu J, Aksay IA, Lin Y (2010) Graphene based electrochemical sensors and biosensors: a review. Electroanal 22:1027–1036

    Article  CAS  Google Scholar 

  7. Wang J (2005) Carbon-nanotube based electrochemical biosensors: a review. Electroanal 17:7–14

    Article  CAS  Google Scholar 

  8. Villalonga R, Díez P, Casado S, Eguílaz M, Yáñez-Sedeño P, Pingarrón JM (2012) Electropolymerized network of polyamidoamine dendron-coated gold nanoparticles as novel nanostructured electrode surface for biosensor construction. Analyst 137:342–348

    Article  CAS  Google Scholar 

  9. Zhu N, Gao H, Gu Y, Xu Q, He P, Fang Y (2009) PAMAM dendrimer-enhanced DNA biosensors based on electrochemical impedance spectroscopy. Analyst 134:860–866

    Article  CAS  Google Scholar 

  10. Vögtle F, Richardt G, Werner N (2009) Dendrimer chemistry: concepts, synthesis, properties, applications. Wiley–VCH, Weinheim

  11. Endo T, Yoshimura T, Esumi K (2005) Synthesis and catalytic activity of gold–silver binary nanoparticles stabilized by PAMAM dendrimer. J Colloid Interface Sci 286:602–609

    Article  CAS  Google Scholar 

  12. Crooks RM, Zhao M, Sun L, Chechik V, Yeung LK (2001) Dendrimer-encapsulated metal nanoparticles: synthesis, characterization, and applications to catalysis. Acc Chem Res 34:181–190

    Article  CAS  Google Scholar 

  13. Xu L, Zhu Y, Tang L, Yang X, Li C (2007) Biosensor based on self-assembling glucose oxidase and dendrimer-encapsulated Pt nanoparticles on carbon nanotubes for glucose detection. Electroanal 19:717–722

    Article  CAS  Google Scholar 

  14. Zhu Y, Zhu H, Yang X, Xu L, Li C (2007) Sensitive biosensors based on (dendrimer encapsulated Pt nanoparticles)/enzyme multilayers. Electroanal 19:698–703

    Article  CAS  Google Scholar 

  15. Villalonga R, Cao R, Fragoso A (2007) Supramolecular chemistry of cyclodextrins in enzyme technology. Chem Rev 107:3088–3116

    Article  CAS  Google Scholar 

  16. Holzinger M, Bouffier L, Villalonga R, Cosnier S (2009) Adamantane/β-cyclodextrin affinity biosensors based on single-walled carbon nanotubes. Biosens Bioelectron 24:1128–1134

    Article  CAS  Google Scholar 

  17. Villalonga R, Camacho C, Cao R, Hernández J, Matías JC (2007) Amperometric biosensor for xanthine with supramolecular architecture. Chem Commun 942–944

  18. Villalonga R, Matos M, Cao R (2007) Construction of an amperometric biosensor for xanthine via supramolecular associations. Electrochem Commun 9:454–458

    Article  CAS  Google Scholar 

  19. Wang J (2008) Electrochemical glucose biosensors. Chem Rev 108:814–825

    Article  CAS  Google Scholar 

  20. Cakara D, Kleimann J, Borkovec M (2003) Microscopic protonation equilibria of poly(amidoamine) dendrimers from macroscopic titrations. Macromolecules 36:4201–4207

    Article  CAS  Google Scholar 

  21. Katsounaros I, Schneider WB, Meier JC, Benedikt U, Biedermann PU, Auer AA, Mayrhofer KJJ (2012) Hydrogen peroxide electrochemistry on platinum: towards understanding the oxygen reduction reaction mechanism. Phys Chem Chem Phys 14:7384–7391

    Article  CAS  Google Scholar 

  22. Swoboda BEP, Massey V (1965) Purification and properties of glucose oxidase from Aspergillus niger. J Biol Chem 240:2209–2215

    CAS  Google Scholar 

  23. Wu WC, Huang JL, Tsai YC (2012) Direct electron transfer and biosensing of glucose oxidase immobilized at multiwalled carbon nanotube-alumina-coated silica modified electrode. Mat Sci Eng C 32:983–987

    Article  CAS  Google Scholar 

  24. Deng C, Chen J, Chen X, Xiao C, Nie L, Yao S (2008) Direct electrochemistry of glucose oxidase and biosensing for glucose based on boron-doped carbon nanotubes modified electrode. Biosens Bioelectron 23:1272–1277

    Article  CAS  Google Scholar 

  25. Wang Y, Liu L, Li M, Xu S, Gao F (2011) Multifunctional carbon nanotubes for direct electrochemistry of glucose oxidase and glucose bioassay. Biosens Bioelectron 30:107–111

    Article  CAS  Google Scholar 

  26. Zhang H, Meng Z, Wang Q, Zheng J (2011) A novel glucose biosensor based on direct electrochemistry of glucose oxidase incorporated in biomediated gold nanoparticles–carbon nanotubes composite film. Sens Actuator B Chem 158:23–27

    Article  CAS  Google Scholar 

  27. Yan XB, Chen XJ, Tay BK, Khor KA (2007) Transparent and flexible glucose biosensor via layer-by-layer assembly of multi-wall carbon nanotubes and glucose oxidase. Electrochem Commun 9:1269–1275

    Article  CAS  Google Scholar 

  28. Li J, Yu J, Zhao F, Zeng B (2007) Direct electrochemistry of glucose oxidase entrapped in nano gold particles-ionic liquid-N, N-dimethylformamide composite film on glassy carbon electrode and glucose sensing. Anal Chim Acta 587:33–40

    Article  CAS  Google Scholar 

  29. Şenel M, Nergiz C (2012) Novel amperometric glucose biosensor based on covalent immobilization of glucose oxidase on poly(pyrrole propylic acid)/Au nanocomposite. Curr Appl Phys 12:1118–1124

    Article  Google Scholar 

  30. Qiu H, Zou F (2012) Fabrication of stratified nanoporous gold for enhanced biosensing. Biosens Bioelectron 35:349–354

    Article  CAS  Google Scholar 

  31. Male KB, Hrapovic S, Luong JHT (2007) Electrochemically-assisted deposition of oxidases on platinum nanoparticle/multi-walled carbon nanotube-modified electrodes. Analyst 132:1254–1261

    Article  CAS  Google Scholar 

  32. Qiu C, Wang X, Liu X, Hou S, Ma H (2012) Direct electrochemistry of glucose oxidase immobilized on nanostructured gold thin films and its application to bioelectrochemical glucose sensor. Electrochim Acta 67:140–146

    Article  CAS  Google Scholar 

  33. Xiao X, Zhou B, Zhu L, Xu L, Tan L, Tang H, Zhang Y, Xie Q, Yao S (2012) An reagentless glucose biosensor based on direct electrochemistry of glucose oxidase immobilized on poly(methylene blue) doped silica nanocomposites. Sens Actuator B Chem 165:126–132

    Article  CAS  Google Scholar 

Download references

Acknowledgements

R. Villalonga acknowledges the Ramón and Cajal contract from the Spanish Ministry of Science and Innovation. Financial support from the Spanish Ministry of Science and Innovation (CTQ2011-24355, CTQ2009-12650, CTQ2009-09351) and Comunidad de Madrid S2009/PPQ-1642, programme AVANSENS, are gratefully acknowledged.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain

    Paula Díez, María Gamella, Reynaldo Villalonga & José M. Pingarrón

  2. Department of Chemical Engineering, “Gh. Asachi” Technical University, Bd. D. Mangeron No. 71A, 700050, Iasi, Romania

    Ciprian-George Piuleac

  3. Department of Organic Chemistry I, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain

    Paloma Martínez-Ruiz & Santiago Romano

Authors
  1. Paula Díez
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  2. Ciprian-George Piuleac
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  3. Paloma Martínez-Ruiz
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  4. Santiago Romano
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  5. María Gamella
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  6. Reynaldo Villalonga
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  7. José M. Pingarrón
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Corresponding authors

Correspondence to Reynaldo Villalonga or José M. Pingarrón.

Additional information

Published in the topical collection in Bioelectroanalysis with guest editors Nicolas Plumeré, Magdalena Gebala, and Wolfgang Schuhmann.

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Díez, P., Piuleac, CG., Martínez-Ruiz, P. et al. Supramolecular immobilization of glucose oxidase on gold coated with cyclodextrin-modified cysteamine core PAMAM G-4 dendron/Pt nanoparticles for mediatorless biosensor design. Anal Bioanal Chem 405, 3773–3781 (2013). https://doi.org/10.1007/s00216-012-6491-8

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  • DOI: https://doi.org/10.1007/s00216-012-6491-8

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